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Home / Equine Research Bank / Folia Microbiol (Praha) / Fecal coagulase-negative staphylococci from horses, their sp...
Folia microbiologica2019; 64(6); 719-726; doi: 10.1007/s12223-019-00684-5

Fecal coagulase-negative staphylococci from horses, their species variability, and biofilm formation.

Abstract: The intestinal microbiota has enormous impact on the health and performance of horses. Staphylococci belong in the phylum Firmicutes, and their occurrence, especially of methicillin-resistant strains and species, has been reported in horses previously. Moreover, biofilm formation is one of the virulence factors; it has been not completely studied in fecal coagulase-negative staphylococci (CoNS) from horses. Therefore, this study was focused on biofilm formation by various species of fecal CoNS from horses because it has been never reported before. In addition, their antibiotic profile was tested. Horses (42) of various breeds from Slovakia/Poland were sampled. Variability in the species of CoNS was detected in feces of horses. Thirty-two strains were identified by using the MALDI-TOF system and classified into nine species and three subspecies of CoNS: Staphylococcus capitis, S. cohnii subsp. cohnii, S. cohnii subsp. urealyticus, S. cohnii subsp. casei, S. epidermidis, S. haemolyticus, S. pasteuri, S. sciuri, S. vitulinus, S. warneri, and S. xylosus. The most frequent species was S. vitulinus. Twenty-two strains showed high biofilm production; 10 strains showed low-grade biofilm production. The highest biofilm formation was measured in the species S. xylosus. Eleven strains (of 32) were methicillin-resistant; the others were susceptible to methicillin.
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Publication Date: 2019-01-31 PubMed ID: 30706301DOI: 10.1007/s12223-019-00684-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This article investigates the diversity of species of fecal staphylococci bacteria in horses, their capacity to develop biofilms, and their antibiotic resistance profiles.

Objective of the Study

  • The purpose of this study was to gain insight into the different species of fecal coagulase-negative staphylococci (CoNS) present in horses and the extent to which they can form biofilms. This is important since biofilms, structured communities of bacteria enclosed within a self-produced matrix, contribute to bacterial virulence. Another objective was to determine the bacteria’s resistance to methicillin, an antibiotic often used in treating staphylococci infections.

Methodology

  • A total of 42 horses of varying breeds from Slovakia and Poland participated in this study. Fecal samples were collected and analysed for variability in CoNS species.
  • The collected samples were tested for different species of CoNS, using the MALDI-TOF (Matrix Assisted Laser Desorption Ionization – Time of Flight) system, an accurate method for identifying microorganisms.
  • The study also measured the level of biofilm production by the isolated CoNS strains.
  • In addition, the strains’ resistance to methicillin was tested.

Findings

  • Nine different species and three subspecies of CoNS were identified within the samples: Staphylococcus capitis, S. cohnii subsp. cohnii, S. cohnii subsp. urealyticus, S. cohnii subsp. casei, S. epidermidis, S. haemolyticus, S. pasteuri, S. sciuri, S. vitulinus, S. warneri, and S. xylosus.
  • The most common species encountered was S. vitulinus.
  • Regarding biofilm production, about 22 CoNS strains exhibited a high level of biofilm formation, while another 10 showed a low level of biofilm creation.
  • The species S. xylosus was identified with the highest level of biofilm formation.
  • Eleven of the 32 strains studied were found to be resistant to methicillin, while the rest were susceptible.

Cite This Article

APA
Bino E, Lauková A, Ščerbová J, Kubašová I, Kandričáková A, Strompfová V, Miltko R, Belzecki G. (2019). Fecal coagulase-negative staphylococci from horses, their species variability, and biofilm formation. Folia Microbiol (Praha), 64(6), 719-726. https://doi.org/10.1007/s12223-019-00684-5

Publication

Folia microbiologica
ISSN: 1874-9356
NlmUniqueID: 0376757
Country: United States
Language: English
Volume: 64
Issue: 6
Pages: 719-726

Researcher Affiliations

Bino, E
  • Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Lauková, Andrea
  • Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia. laukova@saske.sk.
Ščerbová, J
  • Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Kubašová, I
  • Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Kandričáková, A
  • Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Strompfová, V
  • Institute of Animal Physiology of the Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Miltko, R
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05 110, Jablonna, Poland.
Belzecki, G
  • The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05 110, Jablonna, Poland.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Biofilms / growth & development
  • Coagulase / deficiency
  • Feces / microbiology
  • Gastrointestinal Microbiome
  • Horses
  • Lactic Acid / metabolism
  • Methicillin Resistance
  • Microbial Sensitivity Tests
  • Poland / epidemiology
  • Slovakia / epidemiology
  • Species Specificity
  • Staphylococcal Infections / epidemiology
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / veterinary
  • Staphylococcus / classification
  • Staphylococcus / drug effects
  • Staphylococcus / isolation & purification
  • Staphylococcus / physiology

Grant Funding

  • 2/0006/17 / VEGA, Slovakia
  • 2/0012/16 / VEGA Slovakia

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Citations

This article has been cited 6 times.
  1. Zábolyová N, Lauková A, Pogány Simonová M. Susceptibility to postbiotics - enterocins of methicillin-resistant Staphylococcus aureus strains isolated from rabbits. Vet Res Commun 2024 Jun;48(3):1449-1457.
    doi: 10.1007/s11259-024-10323-1pubmed: 38324077google scholar: lookup
  2. Louail R, Florin F, Bernard S, Michaud JB, Breton J, Achamrah N, Tavolacci MP, Coëffier M, Ribet D. Invasion of intestinal cells by Staphylococcus warneri, a member of the human gut microbiota. Gut Pathog 2023 Jan 27;15(1):4.
    doi: 10.1186/s13099-022-00528-7pubmed: 36707889google scholar: lookup
  3. Silva V, Correia E, Pereira JE, González-Machado C, Capita R, Alonso-Calleja C, Igrejas G, Poeta P. Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species. Pathogens 2022 Jun 16;11(6).
    doi: 10.3390/pathogens11060689pubmed: 35745543google scholar: lookup
  4. Fungwithaya P, Boonchuay K, Narinthorn R, Sontigun N, Sansamur C, Petcharat Y, Thomrongsuwannakij T, Wongtawan T. First study on diversity and antimicrobial-resistant profile of staphylococci in sports animals of Southern Thailand. Vet World 2022 Mar;15(3):765-774.
    doi: 10.14202/vetworld.2022.765-774pubmed: 35497942google scholar: lookup
  5. Silva V, Alfarela C, Caniça M, Manageiro V, Nóvoa M, Leiva B, Kress M, Capelo JL, Poeta P, Igrejas G. A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers. Antibiotics (Basel) 2022 Mar 10;11(3).
    doi: 10.3390/antibiotics11030374pubmed: 35326837google scholar: lookup
  6. Scholtzek AD, Hanke D, Walther B, Eichhorn I, Stöckle SD, Klein KS, Gehlen H, Lübke-Becker A, Schwarz S, Feßler AT. Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility. Toxins (Basel) 2019 Sep 13;11(9).
    doi: 10.3390/toxins11090535pubmed: 31540335google scholar: lookup
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