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Home / Equine Research Bank / Curr Microbiol / Fighting Off Wound Pathogens in Horses with Honeybee Lactic ...
Current microbiology2016; 73(4); 463-473; doi: 10.1007/s00284-016-1080-2

Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria.

Abstract: In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. Therefore, antibiotics are often prescribed. Recent studies have shown that honeybee-specific lactic acid bacteria (LAB), involved in honey production, and inhibit human wound pathogens. The aim of this pilot study was to investigate the effects on the healing of hard-to-heal equine wounds after treatment with these LAB symbionts viable in a heather honey formulation. For this, we included ten horses with wound duration of >1 year, investigated the wound microbiota, and treated wounds with the novel honeybee LAB formulation. We identified the microbiota using MALDI-TOF mass spectrometry and DNA sequencing. In addition, the antimicrobial properties of the honeybee LAB formulation were tested against all wound isolates in vitro. Our results indicate a diverse wound microbiota including fifty-three bacterial species that showed 90 % colonization by at least one species of Staphylococcus. Treatment with the formulation promoted wound healing in all cases already after the first application and the wounds were either completely healed (n = 3) in less than 20 days or healing was in progress. Furthermore, the honeybee LAB formulation inhibited all pathogens when tested in vitro. Consequently, this new treatment option presents as a powerful candidate for the topical treatment of hard-to-heal wounds in horses.
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Publication Date: 2016-06-21 PubMed ID: 27324340PubMed Central: PMC4999459DOI: 10.1007/s00284-016-1080-2Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the use of honeybee lactic acid bacteria in treating persistent, hard-to-heal wounds in horses. The findings indicate that this treatment promotes healing and inhibits the growth of harmful bacteria.

About the Study

  • The researchers carried out a pilot study on ten horses that had wounds for more than a year. This study was undertaken to understand the effects of honeybee lactic acid bacteria on equine wounds, especially those that are hard to heal.
  • The main motive behind this study was to find promising topical antibiotic alternatives that can be effectively used in treating wound infections in humans and animals considering the growing concern over antibiotic resistance.

The Use of Honeybee Lactic Acid Bacteria

  • Honeybee lactic acid bacteria (LAB), known for its role in honey production, was used in this experiment due to its proven properties of inhibiting human wound pathogens.
  • The LAB from honeybees was kept viable in a heather honey formulation and applied on the wounds of the horses.

Analysis and Treatment

  • The wound microbiota in horses was investigated using DNA sequencing and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry.
  • The study revealed a diverse wound microbiota, which included fifty-three bacterial species, with at least one species of staphylococcus colonizing 90% of them.
  • The wounds were treated with the honeybee LAB formulation which in turn revealed positive results after only the first application.

Results of the Study

  • A notable result of the treatment was that it stimulated healing in all the cases. In less than 20 days, some wounds (n=3) were completely healed, while others were in the process of healing.
  • Moreover, when tested in vitro, the honeybee LAB formulation was able to inhibit all wound isolates.
  • The promising results suggest that this new treatment alternative presents a strong candidate for the topical treatment of hard-to-heal wounds in horses.

Cite This Article

APA
Olofsson TC, Butler É, Lindholm C, Nilson B, Michanek P, Vásquez A. (2016). Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria. Curr Microbiol, 73(4), 463-473. https://doi.org/10.1007/s00284-016-1080-2

Publication

Current microbiology
ISSN: 1432-0991
NlmUniqueID: 7808448
Country: United States
Language: English
Volume: 73
Issue: 4
Pages: 463-473

Researcher Affiliations

Olofsson, Tobias C
  • Laboratory Medicine, Lunds Universitet, Lund, Sweden. tobias.olofsson@med.lu.se.
Butler, Éile
  • Laboratory Medicine, Lunds Universitet, Lund, Sweden.
Lindholm, Christina
  • Division of Nursing Science, Sophiahemmet Hogskola, Stockholm, Sweden.
Nilson, Bo
  • Laboratory Medicine, Clinical Microbiology, Region Skåne, Lund, Sweden.
  • Department of Laboratory Medicine Lund, Medical Microbiology, Lund University, Sölvegatan 23, 22362, Lund, Sweden.
Michanek, Per
  • Animal Farm Veterinary Consultants, Degebergavägen, 27568, Vollsjö, Sweden.
Vásquez, Alejandra
  • Laboratory Medicine, Lunds Universitet, Lund, Sweden.

MeSH Terms

  • Animals
  • Bees / microbiology
  • Bifidobacterium / metabolism
  • Biological Therapy
  • Honey / analysis
  • Honey / microbiology
  • Honey / statistics & numerical data
  • Horse Diseases / microbiology
  • Horse Diseases / physiopathology
  • Horse Diseases / therapy
  • Horses
  • Lactic Acid / metabolism
  • Lactobacillus / metabolism
  • Pilot Projects
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / physiopathology
  • Staphylococcal Infections / therapy
  • Staphylococcal Infections / veterinary
  • Staphylococcus / drug effects
  • Staphylococcus / genetics
  • Staphylococcus / growth & development
  • Staphylococcus / isolation & purification
  • Wound Healing
  • Wounds and Injuries / microbiology
  • Wounds and Injuries / physiopathology
  • Wounds and Injuries / therapy
  • Wounds and Injuries / veterinary

Conflict of Interest Statement

Therefore, the authors declare no competing interests regarding this work.

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