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First Complete Coding Sequence of a Venezuelan Equine Encephalitis Virus Strain Isolated from an Equine Encephalitis Case in Costa Rica.
Abstract: The first complete coding sequence of the Venezuelan equine encephalitis virus IE, isolated from a Costa Rican mare with severe encephalitis, was confirmed by histological and viral whole-genome analyses. The isolated virus grouped in the Pacific cluster.
Copyright © 2019 León et al.
Publication Date: 2019-09-05 PubMed ID: 31488528PubMed Central: PMC6728638DOI: 10.1128/MRA.00672-19Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research article is about the first complete sequence of Venezuelan equine encephalitis virus isolated from a horse in Costa Rica, which was found to belong to the Pacific group of the virus, and its potential implications.
Objective of the Research
The research aimed at isolating and sequencing for the first time the complete code of Venezuelan equine encephalitis virus IE from an equine case. The horse, a mare from Costa Rica, presented severe encephalitis symptoms. The research serves to analyze the genome of the virus for better understanding to promote prevention and control strategies.
- Using histological and whole-genome analyses, the researchers confirmed the presence of the Venezuelan equine encephalitis virus in the infected mare.
- This virus was successfully isolated for further study and characterization.
Methodology of Research
The methodology of the research involved mainly histological and genomic analysis.
- In histological analysis, tissues from the infected horse were analyzed to look for signs of infection and inflammation caused by the virus. This allowed the researchers to confirm that the mare was indeed suffering from encephalitis that was likely caused by the Venezuelan equine encephalitis virus.
- In the genetic analysis, the researchers sequenced the entire genome of the virus. This helped them to understand the genetic make-up of this virus, including any unique features it may possess.
Findings of the Research
The key findings of this research revolved around the isolation and sequencing of the Venezuelan equine encephalitis virus and its subsequent classification into the Pacific group.
- The isolation of the virus allowed the researchers to study it in greater detail. They discovered that it possessed coding sequences similar to other known strains of Venezuelan equine encephalitis virus.
- Genomic analysis revealed that the isolated virus belonged to the Pacific cluster of Venezuelan equine encephalitis viruses. This is significant because it helps to shed light on the distribution and migration of the virus, which in turn can help in the development of better preventative measures and control strategies against the disease.
Significance of the Research
This research is key in the understanding of the exact genetic makeup of the Venezuelan equine encephalitis virus in Costa Rica.
- Having the full coding sequence for this virus allows for a deeper understanding of its characteristics, virulence, and potential for mutation.
- The classification of this virus as part of the Pacific cluster allows for better tracking and understanding of the geographical distribution and migration of these viruses.
- With this information, effective prevention and control strategies can be more adequately designed and implemented.
Cite This Article
APA
León B, Jiménez C, González R, Ramirez-Carvajal L.
(2019).
First Complete Coding Sequence of a Venezuelan Equine Encephalitis Virus Strain Isolated from an Equine Encephalitis Case in Costa Rica.
Microbiol Resour Announc, 8(36), e00672-19.
https://doi.org/10.1128/MRA.00672-19 Publication
Researcher Affiliations
- Biosecurity Laboratory (LSE), Animal Health National Service (SENASA), Heredia, Costa Rica bleon@senasa.go.cr lizbeth.ramirez@senasa.go.cr.
- Virology Laboratory, Veterinary Medicine School, Tropical Disease Research Program, Universidad Nacional (UNA), Heredia, Costa Rica.
- Pathology Laboratory, LSE, Animal Health National Service (SENASA), Heredia, Costa Rica.
- Department of Biomedical Sciences, Section of Anatomic Pathology, Cornell University, Ithaca, New York, USA.
- Diagnostic Department, Animal Health National Service (SENASA), Heredia, Costa Rica bleon@senasa.go.cr lizbeth.ramirez@senasa.go.cr.
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Citations
This article has been cited 6 times.- León B, Cordero-Solorzano JM, Rodríguez L, Jiménez C. Vesicular Stomatitis Virus Isolated from a Bovine Brain Sample in Costa Rica. Microbiol Resour Announc 2022 Oct 20;11(10):e0073722.
- León B, González G, Nicoli A, Rojas A, Pizio AD, Ramirez-Carvajal L, Jimenez C. Phylogenetic and Mutation Analysis of the Venezuelan Equine Encephalitis Virus Sequence Isolated in Costa Rica from a Mare with Encephalitis. Vet Sci 2022 May 28;9(6).
- Barrantes Murillo DF, Piche-Ovares M, Gamboa-Solano JC, Romero LM, Soto-Garita C, Alfaro-Alarcón A, Corrales-Aguilar E. Serological Positivity against Selected Flaviviruses and Alphaviruses in Free-Ranging Bats and Birds from Costa Rica Evidence Exposure to Arboviruses Seldom Reported Locally in Humans. Viruses 2022 Jan 6;14(1).
- Ortiz DI, Piche-Ovares M, Romero-Vega LM, Wagman J, Troyo A. The Impact of Deforestation, Urbanization, and Changing Land Use Patterns on the Ecology of Mosquito and Tick-Borne Diseases in Central America. Insects 2021 Dec 23;13(1).
- León B, Jiménez-Sánchez C, Retamosa-Izaguirre M. An Environmental Niche Model to Estimate the Potential Presence of Venezuelan Equine Encephalitis Virus in Costa Rica. Int J Environ Res Public Health 2020 Dec 30;18(1).
- Valles-Morera A, Murillo T, Lizano-Bolaños J, Gutierrez-Roche S, Alvarado M, Alfaro-Alvarado J, Calvo-Salas GA, Prado-Hidalgo G, Ortega J, Corrales-Aguilar E. Exposure to non-endemic arboviruses (alphaviruses) in Costa Rica assessed from human samples collected in areas with contrasting levels of dengue endemicity. Front Public Health 2025;13:1537019.
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