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Parasitology research2020; 119(2); 473-481; doi: 10.1007/s00436-019-06587-1

First report of the isolation and phylogenetic characterization of equine Setaria digitata from India based on mitochondrial COI, 12S rDNA, and nuclear ITS2 sequence data.

Abstract: Equine ocular setariasis arising mainly from ectopic infestation of Setaria digitata is a common vision impairing ophthalmic disease in India, and the identification of this filarial nematode is based solely on morphology. However, morphological characters alone are inadequate to detect and differentiate S. digitata from its congeners. The present communication reports the first phylogenetic characterization of equine S. digitata from India based on sequences derived from the mitochondrial cytochrome c oxidase subunit 1 (COI), the mitochondrial small subunit ribosomal DNA (12S rDNA), and the nuclear internal transcribed spacer 2 (ITS2). Three isolates were characterized for each gene, and respective sequences were submitted to NCBI database (MN078131, MN078132, and MN095798). The sequences were also compared with the other related sequences available from PubMed around the globe, and phylogenetic analysis was carried out in conjunction with nucleotide homologies. There was no intraspecific variation among the Indian isolates. The phylogenetic analysis of S. digitata, inferred from these genes, showed that the isolate sequences obtained from different host species created a separate monophyletic clade within the genus Setaria with minor sequence variations revealing similar molecular characteristics of S. digitata isolates throughout the globe. In addition, the studied Indian isolates were found closer to Sri Lankan isolates. The S. digitata and S. labiatopapillosa appeared as sister species.
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Publication Date: 2020-01-02 PubMed ID: 31897790DOI: 10.1007/s00436-019-06587-1Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study presents the first phylogenetic characterization of the equine Setaria digitata (a type of parasitic worm) from India using genetic information. The characterization was based on genetic analyses of the worm’s mitochondrial and nuclear sequences, revealing that there are only minor variations between Indian isolates and those seen globally. This could help in better understanding and treating diseases caused by this parasite.

Understanding the Research Topic

  • The study focuses on a worm called Setaria digitata, a parasite that infests horses (equine) that can cause vision problems. These infections are common in India.
  • The problem is that it has been difficult to identify and differentiate this parasite from similar species based on morphology (the physical appearance and characteristics of organisms) alone.
  • This is the first study to use gene sequencing to identify Setaria digitata from India, providing more accurate and specific identification.

Methodology

  • Cytochrome c oxidase subunit 1 (COI), small subunit ribosomal DNA (12S rDNA), and the nuclear internal transcribed spacer 2 (ITS2) sequences of the parasite were analyzed – these are part of the parasite’s mitochondrial and nuclear genetic code.
  • Three isolates, independent samples of the organism, were characterized for each gene.
  • The results were submitted to the NCBI database, an online database of genetic information, for further verification and comparison with global data.

Results and Discovery

  • There was no variation in the genetic sequences within the Indian isolates – this shows that the Setaria digitata in India are very similar to each other at the genetic level.
  • When compared with global data, it was discovered that the genetic sequences of Setaria digitata from different hosts formed a distinct monophyletic clade, meaning they all descended from a common ancestor.
  • The study also discovered minor sequence variation between the Indian isolates and global isolates, highlighting their close genetic similarities.
  • Interestingly, the Indian isolates were found to be most genetically similar to isolates from Sri Lanka.

Implications

  • The genetic characterization of Setaria digitata provides a more accurate method of differentiating this species from its relatives. This can help in developing more specific treatments for infections caused by this parasite.
  • The close genetic relationship between Indian and Sri Lankan isolates could indicate a common ancestral genetic pathway or similar environmental influences on the parasite’s evolution.

Cite This Article

APA
Maharana BR, Potliya S, Ganguly A, Bisla RS, Mishra C, Ganguly I. (2020). First report of the isolation and phylogenetic characterization of equine Setaria digitata from India based on mitochondrial COI, 12S rDNA, and nuclear ITS2 sequence data. Parasitol Res, 119(2), 473-481. https://doi.org/10.1007/s00436-019-06587-1

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 119
Issue: 2
Pages: 473-481

Researcher Affiliations

Maharana, B R
  • Regional Centre, Lala Lajpat Rai University of Veterinary and Animal Sciences, Karnal, Haryana, 132001, India.
Potliya, S
  • Regional Centre, Lala Lajpat Rai University of Veterinary and Animal Sciences, Karnal, Haryana, 132001, India.
Ganguly, A
  • Regional Centre, Lala Lajpat Rai University of Veterinary and Animal Sciences, Karnal, Haryana, 132001, India. anitaganguly@gmail.com.
Bisla, R S
  • Regional Centre, Lala Lajpat Rai University of Veterinary and Animal Sciences, Karnal, Haryana, 132001, India.
Mishra, C
  • Department of Animal Breeding and Genetics, OUAT, Bhubaneswar, Odisha, 751003, India.
Ganguly, I
  • ICAR-NBAGR, Karnal, Haryana, 132001, India.

MeSH Terms

  • Animals
  • DNA, Intergenic / genetics
  • DNA, Ribosomal / genetics
  • Electron Transport Complex IV / genetics
  • Eye Diseases / parasitology
  • Eye Diseases / veterinary
  • Filarioidea / genetics
  • Filarioidea / isolation & purification
  • Horse Diseases / parasitology
  • Horses / parasitology
  • India
  • Phylogeny
  • Polymerase Chain Reaction / veterinary
  • RNA, Ribosomal / genetics
  • Sequence Analysis, DNA
  • Setaria Nematode / genetics
  • Setaria Nematode / isolation & purification
  • Setariasis / parasitology

References

This article includes 29 references
  1. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.. Mol Biol Evol 2018 Jun 1;35(6):1547-1549.
    pubmed: 29722887doi: 10.1093/molbev/msy096google scholar: lookup
  2. Wijesundera WS, Chandrasekharan NV, Karunanayake EH, Dharmasena SP. Development of a diagnostic DNA probe to detect Setaria digitata: the causative parasite of cerebrospinal nematodiasis in goats, sheep and horses.. Br Vet J 1996 Sep;152(5):561-71.
    pubmed: 8885466doi: 10.1016/s0007-1935(96)80008-xgoogle scholar: lookup
  3. Zardoya R, Meyer A. Phylogenetic performance of mitochondrial protein-coding genes in resolving relationships among vertebrates.. Mol Biol Evol 1996 Sep;13(7):933-42.
    pubmed: 8752002doi: 10.1093/oxfordjournals.molbev.a025661google scholar: lookup
  4. Perumal AN, Gunawardene YI, Dassanayake RS. Setaria digitata in advancing our knowledge of human lymphatic filariasis.. J Helminthol 2016 Mar;90(2):129-38.
    pubmed: 25924635doi: 10.1017/S0022149X15000309google scholar: lookup
  5. Chabaud AG, Bain O. The evolutionary expansion of the Spirurida.. Int J Parasitol 1994 Dec;24(8):1179-201.
    pubmed: 7729976doi: 10.1016/0020-7519(94)90190-2google scholar: lookup
  6. Sudan V, Kumar R, Shanker D, Paliwal S. First report of molecular characterization and phylogenetic analysis of Sarcocystis tenella from India.. Parasitol Res 2019 May;118(5):1429-1434.
    pubmed: 30868294doi: 10.1007/s00436-019-06282-1google scholar: lookup
  7. Casiraghi M, Anderson TJ, Bandi C, Bazzocchi C, Genchi C. A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts.. Parasitology 2001 Jan;122 Pt 1:93-103.
    pubmed: 11197770doi: 10.1017/s0031182000007149google scholar: lookup
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees.. Mol Biol Evol 1987 Jul;4(4):406-25.
    pubmed: 3447015doi: 10.1093/oxfordjournals.molbev.a040454google scholar: lookup
  9. Chamuah JK, Sakhrie A, Lama S, Chandra S, Chigure GM, Bauri RK, Jacob SS. Molecular characterization of Setaria digitata from Mithun (Bos frontalis).. Acta Parasitol 2015 Sep;60(3):391-4.
    pubmed: 26204175doi: 10.1515/ap-2015-0054google scholar: lookup
  10. Wijesundera WS, Chandrasekharan NV, Karunanayake EH. A sensitive polymerase chain reaction based assay for the detection of Setaria digitata: the causative organism of cerebrospinal nematodiasis in goats, sheep and horses.. Vet Parasitol 1999 Mar 1;81(3):225-33.
    pubmed: 10190866doi: 10.1016/s0304-4017(98)00248-9google scholar: lookup
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.. Mol Biol Evol 2013 Dec;30(12):2725-9.
    pubmed: 24132122doi: 10.1093/molbev/mst197google scholar: lookup
  12. Huang H, Wang T, Yang G, Zhang Z, Wang C, Yang Z, Luo L, Liu L, Lan J, Huang X. Molecular characterization and phylogenetic analysis of Dirofilaria immitis of China based on COI and 12S rDNA genes.. Vet Parasitol 2009 Mar 9;160(1-2):175-9.
    pubmed: 19046806doi: 10.1016/j.vetpar.2008.10.053google scholar: lookup
  13. Rhee JK, Choi EY, Park BK, Jang BG. Application of scanning electron microscopy in assessing the prevalence of some Setaria species in Korean cattle.. Korean J Parasitol 1994 Mar;32(1):1-6.
    pubmed: 8167102doi: 10.3347/kjp.1994.32.1.1google scholar: lookup
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.. Nucleic Acids Res 1997 Dec 15;25(24):4876-82.
    pubmed: 9396791doi: 10.1093/nar/25.24.4876google scholar: lookup
  15. Engelmann JC, Rahmann S, Wolf M, Schultz J, Fritzilas E, Kneitz S, Dandekar T, Müller T. Modelling cross-hybridization on phylogenetic DNA microarrays increases the detection power of closely related species.. Mol Ecol Resour 2009 Jan;9(1):83-93.
    pubmed: 21564570doi: 10.1111/j.1755-0998.2008.02199.xgoogle scholar: lookup
  16. Sundar ST, D'Souza PE. Morphological characterization of Setaria worms collected from cattle.. J Parasit Dis 2015 Sep;39(3):572-6.
    pubmed: 26345074doi: 10.1007/s12639-013-0399-xgoogle scholar: lookup
  17. Jayasinghe DR, Wijesundera WS. Differentiation of Setaria digitata and Setaria labiatopapillosa using molecular markers.. Vet J 2003 Mar;165(2):136-42.
    pubmed: 12573602doi: 10.1016/s1090-0233(02)00157-0google scholar: lookup
  18. Xie H, Bain O, Williams SA. Molecular phylogenetic studies on filarial parasites based on 5S ribosomal spacer sequences.. Parasite 1994 Jun;1(2):141-51.
    pubmed: 9140481doi: 10.1051/parasite/1994012141google scholar: lookup
  19. Yatawara L, Wickramasinghe S, Nagataki M, Rajapakse RP, Agatsuma T. Molecular characterization and phylogenetic analysis of Setaria digitata of Sri Lanka based on CO1 and 12S rDNA genes.. Vet Parasitol 2007 Sep 1;148(2):161-5.
    pubmed: 17614204doi: 10.1016/j.vetpar.2007.06.005google scholar: lookup
  20. Schultz J, Wolf M. ITS2 sequence-structure analysis in phylogenetics: a how-to manual for molecular systematics.. Mol Phylogenet Evol 2009 Aug;52(2):520-3.
    pubmed: 19489124doi: 10.1016/j.ympev.2009.01.008google scholar: lookup
  21. Casiraghi M, Bain O, Guerrero R, Martin C, Pocacqua V, Gardner SL, Franceschi A, Bandi C. Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution.. Int J Parasitol 2004 Feb;34(2):191-203.
    pubmed: 15037105doi: 10.1016/j.ijpara.2003.10.004google scholar: lookup
  22. Radwan AM, Ahmed NE, Elakabawy LM, Ramadan MY, Elmadawy RS. Prevalence and pathogenesis of some filarial nematodes infecting donkeys in Egypt.. Vet World 2016 Aug;9(8):888-92.
    pubmed: 27651679doi: 10.14202/vetworld.2016.888-892google scholar: lookup
  23. Yao H, Song J, Liu C, Luo K, Han J, Li Y, Pang X, Xu H, Zhu Y, Xiao P, Chen S. Use of ITS2 region as the universal DNA barcode for plants and animals.. PLoS One 2010 Oct 1;5(10).
    pubmed: 20957043doi: 10.1371/journal.pone.0013102google scholar: lookup
  24. Shin SS, Cho KO, Wee SH. Ocular infection of cattle with Setaria digitata.. J Vet Med Sci 2002 Jan;64(1):7-10.
    pubmed: 11853150doi: 10.1292/jvms.64.7google scholar: lookup
  25. Bowles J, Blair D, McManus DP. A molecular phylogeny of the human schistosomes.. Mol Phylogenet Evol 1995 Jun;4(2):103-9.
    pubmed: 7663756doi: 10.1006/mpev.1995.1011google scholar: lookup
  26. Kaur D, Ganai A, Parveen S, Borkataki S, Yadav A, Katoch R, Godara R. Occurrence of Setaria digitata in a cow.. J Parasit Dis 2015 Sep;39(3):477-8.
    pubmed: 26345055doi: 10.1007/s12639-013-0376-4google scholar: lookup
  27. Liu GH, Li JY, Zhu XQ. Characterization of the complete mitochondrial genome of Setaria digitata (Nematoda: Setariidae) from China.. J Helminthol 2017 Nov;91(6):772-776.
    pubmed: 28100285doi: 10.1017/S0022149X16000912google scholar: lookup
  28. Shin J, Ahn KS, Suh GH, Kim HJ, Jeong HS, Kim BS, Choi E, Shin SS. First Blindness Cases of Horses Infected with Setaria Digitata (Nematoda: Filarioidea) in the Republic of Korea.. Korean J Parasitol 2017 Dec;55(6):667-671.
    pubmed: 29320823doi: 10.3347/kjp.2017.55.6.667google scholar: lookup
  29. Peng TL, Armiladiana MM, Ruhil HH, Maizan M, Choong SS. First report of equine Setaria digitata (von Linstow 1906) infestation in Malaysia.. Vet Parasitol Reg Stud Reports 2019 Aug;17:100310.
    pubmed: 31303218doi: 10.1016/j.vprsr.2019.100310google scholar: lookup

Citations

This article has been cited 7 times.
  1. Abo-Aziza FAM, Hendawy SHM, Abdullah HHAM, El Namaky A, Laidoudi Y, Mediannikov O. Emergent and Neglected Equine Filariosis in Egypt: Species Diversity and Host Immune Response. Pathogens 2022 Aug 27;11(9).
    doi: 10.3390/pathogens11090979pubmed: 36145411google scholar: lookup
  2. Abdullah HHAM, Amanzougaghene N, Dahmana H, Louni M, Raoult D, Mediannikov O. Multiple vector-borne pathogens of domestic animals in Egypt. PLoS Negl Trop Dis 2021 Sep;15(9):e0009767.
    doi: 10.1371/journal.pntd.0009767pubmed: 34587171google scholar: lookup
  3. Yu F, Liu B, Chen S, Yi Z, Liu X, Zhu Y, Li J. First Molecular Confirmation of Equine Ocular Setaria digitata in China. Vet Sci 2021 Mar 28;8(4).
    doi: 10.3390/vetsci8040055pubmed: 33800678google scholar: lookup
  4. M R, Rahi M, Saini P. Zoonotic filariasis and its public health significance: a comprehensive literature review. Front Microbiol 2025;16:1700645.
    doi: 10.3389/fmicb.2025.1700645pubmed: 41321830google scholar: lookup
  5. Helmi TZ, Farida F, Kasturi A, Rahmah EPL, Winaruddin W, Hanafiah M. Unraveling the morphological and molecular profile of Setaria digitata in Aceh cattle. Open Vet J 2025 Jan;15(1):187-197.
    doi: 10.5455/OVJ.2025.v15.i1.18pubmed: 40092195google scholar: lookup
  6. Anandu S, Tanuj GN, Vijayasarathi MK, Manjusha KM, Samanta S, Bandyopadhyay S, Sankar M. Molecular identification and genetic variability of equine and bovine ocular setariasis in india: molecular profiling by mitochondrial genes. Mol Biol Rep 2024 Aug 24;51(1):932.
    doi: 10.1007/s11033-024-09846-2pubmed: 39180621google scholar: lookup
  7. Huggins LG, Atapattu U, Young ND, Traub RJ, Colella V. Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans. BMC Microbiol 2024 Jan 20;24(1):28.
    doi: 10.1186/s12866-023-03159-3pubmed: 38245715google scholar: lookup
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