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Mammalian genome : official journal of the International Mammalian Genome Society1997; 8(8); 569-572; doi: 10.1007/s003359900505

FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11.

Abstract: Three genomic subclones derived from a phage clone containing the equine IGF2 gene were used to FISH map the gene on horse (ECA) and donkey (EAS) metaphase chromosomes. The gene mapped on ECA 12q13 band and is the first locus mapped to this horse chromosome. In donkey the gene mapped very terminal on the long arm of one small submetacentric chromosome that shows almost identical DAPI-banding pattern with ECA12. This is the first locus mapped in donkey genome. Cross species chromosome painting of equine metaphase chromosomes with human Chromosome (Chr) 11-specific probe showed homoeology of this human chromosome with ECA12 and ECA7. The novel ECA12 comparative painting results are thus in accordance with the localization of the equine IGF2 gene. Comparison of the hitherto known physical locations of IGF2 in different species, viz. human, cattle, sheep, horse, and donkey, shows that this gene tends to maintain a terminal location on the chromosome arm.
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Publication Date: 1997-08-01 PubMed ID: 9250862DOI: 10.1007/s003359900505Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses a mapping study of the IGF2 gene in horses and donkeys, highlighting its consistent terminal location on the chromosome arm across various species, including humans.

Mapping of the IGF2 Gene in Horses and Donkeys

  • The study employed three genomic subclones extracted from a phage clone that contains the IGF2 gene specific to horses (equine).
  • Through Fluorescence In Situ Hybridization (FISH), a widely used method for genome mapping, the IGF2 gene was found on the 12q13 band of horse chromosomes, marking the first locus mapped on this horse chromosome (ECA).
  • In donkeys, the IGF2 gene was found to map very terminally on the long arm of a small submetacentric chromosome, which has an almost identical DAPI-banding pattern with the horse chromosome 12.
  • This finding also marks the first locus mapped on the donkey genome (EAS).

Cross-Species Chromosome Painting

  • A technique called “chromosome painting” was used to identify homoeology – the condition of being homoeologous – between human Chromosome 11 and horse chromosomes ECA7 and ECA12.
  • Homoeologous chromosomes are similar in terms of structure and gene content but originate from different ancestral species. This suggests that the horse chromosomes ECA12 and ECA7 share a common ancestor with human Chromosome 11.
  • The finding reaffirms the location of the IGF2 gene on ECA12 given the mapping results.

Comparison of IGF2 Gene Locations Across Species

  • Comparing the physical locations of the IGF2 gene across multiple species, such as humans, cattle, sheep, horse, and donkey, showed that this particular gene tends to be located terminally on the chromosome arm.
  • This consistent positioning across different species suggests that the IGF2 gene has preserved its location throughout evolutionary development.

Cite This Article

APA
Raudsepp T, Otte K, Rozell B, Chowdhary BP. (1997). FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11. Mamm Genome, 8(8), 569-572. https://doi.org/10.1007/s003359900505

Publication

Mammalian genome : official journal of the International Mammalian Genome Society
ISSN: 0938-8990
NlmUniqueID: 9100916
Country: United States
Language: English
Volume: 8
Issue: 8
Pages: 569-572

Researcher Affiliations

Raudsepp, T
  • Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, S-750 07 Uppsala, Sweden.
Otte, K
    Rozell, B
      Chowdhary, B P

        MeSH Terms

        • Animals
        • Blotting, Southern
        • Chromosome Mapping
        • Chromosomes / genetics
        • Equidae / genetics
        • Horses / genetics
        • In Situ Hybridization, Fluorescence
        • Insulin-Like Growth Factor II / genetics
        • Karyotyping
        • Metaphase

        References

        This article includes 26 references
        1. Oakenfull EA, Buckle VJ, Clegg JB. Localization of the horse (Equus caballus) alpha-globin gene complex to chromosome 13 by fluorescence in situ hybridization.. Cytogenet Cell Genet 1993;62(2-3):136-8.
          pubmed: 8428512doi: 10.1159/000133456google scholar: lookup
        2. Chowdhary BP, Frönicke L, Gustavsson I, Scherthan H. Comparative analysis of the cattle and human genomes: detection of ZOO-FISH and gene mapping-based chromosomal homologies.. Mamm Genome 1996 Apr;7(4):297-302.
          pubmed: 8661702doi: 10.1007/s003359900086google scholar: lookup
        3. Morison IM, Becroft DM, Taniguchi T, Woods CG, Reeve AE. Somatic overgrowth associated with overexpression of insulin-like growth factor II.. Nat Med 1996 Mar;2(3):311-6.
          pubmed: 8612230doi: 10.1038/nm0396-311google scholar: lookup
        4. Gu F, Chowdhary BP, Johansson M, Andersson L, Gustavsson I. Localization of the IGHG, PRKACB, and TNP2 genes in pigs by in situ hybridization.. Mamm Genome 1994 Apr;5(4):195-8.
          pubmed: 8012108doi: 10.1007/BF00360544google scholar: lookup
        5. Solinas-Toldo S, Lengauer C, Fries R. Comparative genome map of human and cattle.. Genomics 1995 Jun 10;27(3):489-96.
          pubmed: 7558031doi: 10.1006/geno.1995.1081google scholar: lookup
        6. DeChiara TM, Efstratiadis A, Robertson EJ. A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting.. Nature 1990 May 3;345(6270):78-80.
          pubmed: 2330056doi: 10.1038/345078a0google scholar: lookup
        7. O'Brien SJ, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland NG. Anchored reference loci for comparative genome mapping in mammals.. Nat Genet 1993 Feb;3(2):103-12.
          pubmed: 8499943doi: 10.1038/ng0293-103google scholar: lookup
        8. Ansari HA, Hediger R, Fries R, Stranzinger G. Chromosomal localization of the major histocompatibility complex of the horse (ELA) by in situ hybridization.. Immunogenetics 1988;28(5):362-4.
          pubmed: 3169882doi: 10.1007/BF00364235google scholar: lookup
        9. Hayes H. Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes.. Cytogenet Cell Genet 1995;71(2):168-74.
          pubmed: 7656590doi: 10.1159/000134100google scholar: lookup
        10. Viersbach R, Schwanitz G, Nöthen MM. Delineation of marker chromosomes by reverse chromosome painting using only a small number of DOP-PCR amplified microdissected chromosomes.. Hum Genet 1994 Jun;93(6):663-7.
          pubmed: 8005590doi: 10.1007/BF00201567google scholar: lookup
        11. Banerjee S, Smallwood A. A chromatin model of IGF2/H19 imprinting.. Nat Genet 1995 Nov;11(3):237-8.
          pubmed: 7581444doi: 10.1038/ng1195-237google scholar: lookup
        12. Harbitz I, Chowdhary BP, Saether H, Hauge JG, Gustavsson I. A porcine genomic glucosephosphate isomerase probe detects a multiallelic restriction fragment length polymorphism assigned to chromosome 10pter in horse.. Hereditas 1990;112(2):151-6.
          pubmed: 1973160doi: 10.1111/j.1601-5223.1990.tb00052.xgoogle scholar: lookup
        13. Feil R, Walter J, Allen ND, Reik W. Developmental control of allelic methylation in the imprinted mouse Igf2 and H19 genes.. Development 1994 Oct;120(10):2933-43.
          pubmed: 7607083doi: 10.1242/dev.120.10.2933google scholar: lookup
        14. Ohlsson R, Nyström A, Pfeifer-Ohlsson S, Töhönen V, Hedborg F, Schofield P, Flam F, Ekström TJ. IGF2 is parentally imprinted during human embryogenesis and in the Beckwith-Wiedemann syndrome.. Nat Genet 1993 May;4(1):94-7.
          pubmed: 8513333doi: 10.1038/ng0593-94google scholar: lookup
        15. Gu F, Harbitz I, Chowdhary BP, Chaudhary R, Gustavsson I. Localization of the 6-phosphogluconate dehydrogenase (PGD) gene in horses by in situ hybridization.. Hereditas 1992;117(1):93-5.
          pubmed: 1399707doi: 10.1111/j.1601-5223.1992.tb00012.xgoogle scholar: lookup
        16. Bradley RD, Wichman HA. Rapidly evolving repetitive DNAs in a conservative genome: a test of factors that affect chromosomal evolution.. Chromosome Res 1994 Sep;2(5):354-60.
          pubmed: 7981939doi: 10.1007/BF01552794google scholar: lookup
        17. Otte K, Engström W. Insulin-like growth factor II in the horse: determination of a cDNA nucleotide sequence and expression in fetal and adult tissue.. Gen Comp Endocrinol 1994 Nov;96(2):270-5.
          pubmed: 7851727doi: 10.1006/gcen.1994.1182google scholar: lookup
        18. DeChiara TM, Robertson EJ, Efstratiadis A. Parental imprinting of the mouse insulin-like growth factor II gene.. Cell 1991 Feb 22;64(4):849-59.
          pubmed: 1997210doi: 10.1016/0092-8674(91)90513-xgoogle scholar: lookup
        19. Junien C, van Heyningen V. Report of the committee on the genetic constitution of chromosome 11.. Cytogenet Cell Genet 1990;55(1-4):153-69.
          pubmed: 2073829doi: 10.1159/000133007google scholar: lookup
        20. Raudsepp T, Frönicke L, Scherthan H, Gustavsson I, Chowdhary BP. Zoo-FISH delineates conserved chromosomal segments in horse and man.. Chromosome Res 1996 Apr;4(3):218-25.
          pubmed: 8793207doi: 10.1007/BF02254963google scholar: lookup
        21. Schmutz SM, Moker JS, Gallagher DS Jr, Kappes SM, Womack JE. In situ hybridization mapping of LDHA and IGF2 to cattle chromosome 29.. Mamm Genome 1996 Jun;7(6):473.
          pubmed: 8662241doi: 10.1007/s003359900143google scholar: lookup
        22. Higgins MJ, Smilinich NJ, Sait S, Koenig A, Pongratz J, Gessler M, Richard CW 3rd, James MR, Sanford JP, Kim BW. An ordered NotI fragment map of human chromosome band 11p15.. Genomics 1994 Sep 1;23(1):211-22.
          pubmed: 7829073doi: 10.1006/geno.1994.1479google scholar: lookup
        23. Chowdhary BP, Thomsen PD, Harbitz I, Landset M, Gustavsson I. Precise localization of the genes for glucose phosphate isomerase (GPI), calcium release channel (CRC), hormone-sensitive lipase (LIPE), and growth hormone (GH) in pigs, using nonradioactive in situ hybridization.. Cytogenet Cell Genet 1994;67(3):211-4.
          pubmed: 8062599doi: 10.1159/000133825google scholar: lookup
        24. Frönicke L, Chowdhary BP, Scherthan H, Gustavsson I. A comparative map of the porcine and human genomes demonstrates ZOO-FISH and gene mapping-based chromosomal homologies.. Mamm Genome 1996 Apr;7(4):285-90.
          pubmed: 8661700doi: 10.1007/s003359900084google scholar: lookup
        25. Ansari HA, Pearce PD, Maher DW, Broad TE. Regional assignment of conserved reference loci anchors unassigned linkage and syntenic groups to ovine chromosomes.. Genomics 1994 Dec;24(3):451-5.
          pubmed: 7536182doi: 10.1006/geno.1994.1652google scholar: lookup
        26. Chowdhary BP, Harbitz I, Davies W, Gustavsson I. Localization of the calcium release channel gene in cattle and horse by in situ hybridization: evidence of a conserved synteny with glucose phosphate isomerase.. Anim Genet 1992;23(1):43-50.
          pubmed: 1315127

        Citations

        This article has been cited 6 times.
        1. Raudsepp T, Christensen K, Chowdhar BP. Cytogenetics of donkey chromosomes: nomenclature proposal based on GTG-banded chromosomes and depiction of NORs and telomeric sites.. Chromosome Res 2000;8(8):659-70.
          doi: 10.1023/a:1026707002538pubmed: 11196129google scholar: lookup
        2. Caetano AR, Shiue YL, Lyons LA, O'Brien SJ, Laughlin TF, Bowling AT, Murray JD. A comparative gene map of the horse (Equus caballus).. Genome Res 1999 Dec;9(12):1239-49.
          doi: 10.1101/gr.9.12.1239pubmed: 10613847google scholar: lookup
        3. Floyd PD, Li L, Rubakhin SS, Sweedler JV, Horn CC, Kupfermann I, Alexeeva VY, Ellis TA, Dembrow NC, Weiss KR, Vilim FS. Insulin prohormone processing, distribution, and relation to metabolism in Aplysia californica.. J Neurosci 1999 Sep 15;19(18):7732-41.
          doi: 10.1523/JNEUROSCI.19-18-07732.1999pubmed: 10479677google scholar: lookup
        4. Raudsepp T, Chowdhary BP. Construction of chromosome-specific paints for meta- and submetacentric autosomes and the sex chromosomes in the horse and their use to detect homologous chromosomal segments in the donkey.. Chromosome Res 1999;7(2):103-14.
          doi: 10.1023/a:1009234814635pubmed: 10328622google scholar: lookup
        5. Engström W, Shokrai A, Otte K, Granérus M, Gessbo A, Bierke P, Madej A, Sjölund M, Ward A. Transcriptional regulation and biological significance of the insulin like growth factor II gene.. Cell Prolif 1998 Oct-Dec;31(5-6):173-89.
          doi: 10.1111/j.1365-2184.1998.tb01196.xpubmed: 9925986google scholar: lookup
        6. Lindgren G, Sandberg K, Persson H, Marklund S, Breen M, Sandgren B, Carlstén J, Ellegren H. A primary male autosomal linkage map of the horse genome.. Genome Res 1998 Sep;8(9):951-66.
          doi: 10.1101/gr.8.9.951pubmed: 9750194google scholar: lookup
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