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Home / Equine Research Bank / Tissue Eng Part C Methods / Fixation of Hydrogel Constructs for Cartilage Repair in the ...
Tissue engineering. Part C, Methods2017; 23(11); 804-814; doi: 10.1089/ten.TEC.2017.0200

Fixation of Hydrogel Constructs for Cartilage Repair in the Equine Model: A Challenging Issue.

Abstract: To report on the experiences with the use of commercial and autologous fibrin glue (AFG) and of an alternative method based on a 3D-printed polycaprolactone (PCL) anchor for the fixation of hydrogel-based scaffolds in an equine model for cartilage repair. In a first study, three different hydrogel-based materials were orthotopically implanted in nine horses for 1-4 weeks in 6 mm diameter full-thickness cartilage defects in the medial femoral trochlear ridge and fixated with commercially available fibrin glue (CFG). One defect was filled with CFG only as a control. In a second study, CFG and AFG were compared in an ectopic equine model. The third study compared the efficacy of AFG and a 3D-printed PCL-based osteal anchor for fixation of PCL-reinforced hydrogels in three horses for 2 weeks, with a 4-week follow-up to evaluate integration of bone with the PCL anchor. Short-term scaffold integration and cell infiltration were evaluated by microcomputed tomography and histology as outcome parameters. The first study showed signs of subchondral bone resorption in all defects, including the controls filled with CFG only, with significant infiltration of neutrophils. Ectopically, CFG induced clear inflammation with strong neutrophil accumulation; AFG was less reactive, showing fibroblast infiltration only. In the third study the fixation potential for PCL-reinforced hydrogels of AFG was inferior to the PCL anchor. PCL reinforcement had disappeared from two defects and showed signs of dislodging in the remaining four. All six constructs fixated with the PCL anchor were still in place after 2 weeks. At 4 weeks, the PCL anchor showed good integration and signs of new bone formation. The use of AFG should be preferred to xenogeneic products in the horse, but AFG is subject to individual variations and laborious to make. The PCL anchor provides the best fixation; however, this technique involves the whole osteochondral unit, which entails a different conceptual approach to cartilage repair.
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Publication Date: 2017-08-11 PubMed ID: 28795641PubMed Central: PMC7116030DOI: 10.1089/ten.TEC.2017.0200Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article is about the assessment of different fixation methods for hydrogel-based scaffolds used for cartilage repair in horses. The methods compared were commercial and autologous fibrin glue (AFG), and a 3D-printed polycaprolactone (PCL) anchor.

Studying Hydrogel-Based Scaffolds

  • The study focused on evaluating different methods to fixate hydrogel-based scaffolds used for cartilage repair in an equine (horse) model.
  • Three studies were conducted to test the effectiveness of commercial and autologous fibrin glue (both CFG and AFG respectively) and a 3D-printed polycaprolactone (PCL) anchor.

Comparing Different Methods

  • In the first study, three different hydrogel-based materials were implanted in horses and fixated with commercially available fibrin glue.
  • Results showed subchondral bone resorption in all defects and a significant infiltration of neutrophils, which is a sign of inflammation.
  • In the second study, they compared CFG and AFG in an equine model, finding CFG induced clear inflammation with strong neutrophil accumulation, while AFG was less reactive and allowing only fibroblast infiltration.

Evaluating PCL Anchor Fixation

  • The third study evaluated the effectiveness of AFG and a 3D-printed PCL anchor for fixation of PCL-reinforced hydrogels.
  • The results demonstrated the superior performance of the PCL anchor. All six constructs fixated with the PCL anchor were still in place after 2 weeks.
  • By the 4-week mark, the PCL anchor showed positive signs of integration and new bone formation, indicative of an effective fixation and healing process.

Conclusion

  • While AFG was considered preferable in comparison to xenogeneic products, it was found to be subject to individual variations and laborious to make.
  • In comparison, the PCL anchor technique displayed the best results for fixation, though it involves a different approach to cartilage repair as it encompasses the whole osteochondral unit.

Cite This Article

APA
Mancini IAD, Vindas Bolaños RA, Brommer H, Castilho M, Ribeiro A, van Loon JPAM, Mensinga A, van Rijen MHP, Malda J, van Weeren R. (2017). Fixation of Hydrogel Constructs for Cartilage Repair in the Equine Model: A Challenging Issue. Tissue Eng Part C Methods, 23(11), 804-814. https://doi.org/10.1089/ten.TEC.2017.0200

Publication

Tissue engineering. Part C, Methods
ISSN: 1937-3392
NlmUniqueID: 101466663
Country: United States
Language: English
Volume: 23
Issue: 11
Pages: 804-814

Researcher Affiliations

Mancini, Irina A D
  • 1 Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht, The Netherlands .
Vindas Bolaños, Rafael A
  • 2 Cátedra de Cirugía de Especies Mayores, Escuela de Medicina Veterinaria, Universidad Nacional , Heredia, Costa Rica .
Brommer, Harold
  • 1 Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht, The Netherlands .
Castilho, Miguel
  • 3 Division of Surgery, Department of Orthopaedics, University Medical Center Utrecht , Utrecht, The Netherlands .
  • 4 Department of Biomedical Engineering, Eindhoven University of Technology , Eindhoven, The Netherlands .
Ribeiro, Alexandro
  • 3 Division of Surgery, Department of Orthopaedics, University Medical Center Utrecht , Utrecht, The Netherlands .
van Loon, Johannes P A M
  • 1 Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht, The Netherlands .
Mensinga, Anneloes
  • 3 Division of Surgery, Department of Orthopaedics, University Medical Center Utrecht , Utrecht, The Netherlands .
van Rijen, Mattie H P
  • 3 Division of Surgery, Department of Orthopaedics, University Medical Center Utrecht , Utrecht, The Netherlands .
Malda, Jos
  • 1 Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht, The Netherlands .
  • 3 Division of Surgery, Department of Orthopaedics, University Medical Center Utrecht , Utrecht, The Netherlands .
van Weeren, René
  • 1 Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University , Utrecht, The Netherlands .

MeSH Terms

  • Animals
  • Bone Regeneration / drug effects
  • Cartilage, Articular / diagnostic imaging
  • Cartilage, Articular / pathology
  • Disease Models, Animal
  • Fibrin Tissue Adhesive / pharmacology
  • Horses
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
  • Implants, Experimental
  • Inflammation / pathology
  • Organ Size
  • Polyesters / chemistry
  • Printing, Three-Dimensional
  • Tissue Engineering / methods
  • Tissue Scaffolds / chemistry
  • Wound Healing / drug effects
  • X-Ray Microtomography

Grant Funding

  • 647426 / European Research Council

Conflict of Interest Statement

. No competing financial interests exist.

References

This article includes 52 references
  1. Sophia Fox AJ, Bedi A, Rodeo SA. The basic science of articular cartilage: structure, composition, and function.. Sports Health 2009 Nov;1(6):461-8.
    pmc: PMC3445147pubmed: 23015907doi: 10.1177/1941738109350438google scholar: lookup
  2. Yang Z, Shi Y, Wei X, He J, Yang S, Dickson G, Tang J, Xiang J, Song C, Li G. Fabrication and repair of cartilage defects with a novel acellular cartilage matrix scaffold.. Tissue Eng Part C Methods 2010 Oct;16(5):865-76.
    pubmed: 19891538doi: 10.1089/ten.tec.2009.0444google scholar: lookup
  3. Steinert AF, Ghivizzani SC, Rethwilm A, Tuan RS, Evans CH, Nöth U. Major biological obstacles for persistent cell-based regeneration of articular cartilage.. Arthritis Res Ther 2007;9(3):213.
    pmc: PMC2206353pubmed: 17561986doi: 10.1186/ar2195google scholar: lookup
  4. Temenoff JS, Mikos AG. Review: tissue engineering for regeneration of articular cartilage.. Biomaterials 2000 Mar;21(5):431-40.
    pubmed: 10674807doi: 10.1016/s0142-9612(99)00213-6google scholar: lookup
  5. Smith GD, Knutsen G, Richardson JB. A clinical review of cartilage repair techniques.. J Bone Joint Surg Br 2005 Apr;87(4):445-9.
    pubmed: 15795189doi: 10.1302/0301-620x.87b4.15971google scholar: lookup
  6. Makris EA, Gomoll AH, Malizos KN, Hu JC, Athanasiou KA. Repair and tissue engineering techniques for articular cartilage.. Nat Rev Rheumatol 2015 Jan;11(1):21-34.
    pmc: PMC4629810pubmed: 25247412doi: 10.1038/nrrheum.2014.157google scholar: lookup
  7. Hunziker EB, Lippuner K, Keel MJ, Shintani N. An educational review of cartilage repair: precepts & practice--myths & misconceptions--progress & prospects.. Osteoarthritis Cartilage 2015 Mar;23(3):334-50.
    pubmed: 25534362doi: 10.1016/j.joca.2014.12.011google scholar: lookup
  8. Kuo CK, Li WJ, Mauck RL, Tuan RS. Cartilage tissue engineering: its potential and uses.. Curr Opin Rheumatol 2006 Jan;18(1):64-73.
    pubmed: 16344621doi: 10.1097/01.bor.0000198005.88568.dfgoogle scholar: lookup
  9. Slaughter BV, Khurshid SS, Fisher OZ, Khademhosseini A, Peppas NA. Hydrogels in regenerative medicine.. Adv Mater 2009 Sep 4;21(32-33):3307-29.
    pmc: PMC4494665pubmed: 20882499doi: 10.1002/adma.200802106google scholar: lookup
  10. Kock L, van Donkelaar CC, Ito K. Tissue engineering of functional articular cartilage: the current status.. Cell Tissue Res 2012 Mar;347(3):613-27.
    pmc: PMC3306561pubmed: 22030892doi: 10.1007/s00441-011-1243-1google scholar: lookup
  11. LeBaron RG, Athanasiou KA. Ex vivo synthesis of articular cartilage.. Biomaterials 2000 Dec;21(24):2575-87.
    pubmed: 11071607doi: 10.1016/s0142-9612(00)00125-3google scholar: lookup
  12. Schwab A, Kock L, Mouser VHM, Stichler S, Abbadessa A, Schro¨n F, Hansmann F, Ehlicke F, Walles F. Screening novel hydrogels in an ex vivo cartilage defect model.. Eur Cells Mater 2016;31:7.
  13. McIlwraith CW, Fortier LA, Frisbie DD, Nixon AJ. Equine Models of Articular Cartilage Repair.. Cartilage 2011 Oct;2(4):317-26.
    pmc: PMC4297134pubmed: 26069590doi: 10.1177/1947603511406531google scholar: lookup
  14. Malda J, Benders KE, Klein TJ, de Grauw JC, Kik MJ, Hutmacher DW, Saris DB, van Weeren PR, Dhert WJ. Comparative study of depth-dependent characteristics of equine and human osteochondral tissue from the medial and lateral femoral condyles.. Osteoarthritis Cartilage 2012 Oct;20(10):1147-51.
    pubmed: 22781206doi: 10.1016/j.joca.2012.06.005google scholar: lookup
  15. Hunziker EB, Stähli A. Surgical suturing of articular cartilage induces osteoarthritis-like changes.. Osteoarthritis Cartilage 2008 Sep;16(9):1067-73.
    pmc: PMC2657041pubmed: 18308590doi: 10.1016/j.joca.2008.01.009google scholar: lookup
  16. Bekkers JE, Tsuchida AI, Malda J, Creemers LB, Castelein RJ, Saris DB, Dhert WJ. Quality of scaffold fixation in a human cadaver knee model.. Osteoarthritis Cartilage 2010 Feb;18(2):266-72.
    pubmed: 19800999doi: 10.1016/j.joca.2009.09.001google scholar: lookup
  17. Kerker JT, Leo AJ, Sgaglione NA. Cartilage repair: synthetics and scaffolds: basic science, surgical techniques, and clinical outcomes.. Sports Med Arthrosc Rev 2008 Dec;16(4):208-16.
    pubmed: 19011552doi: 10.1097/jsa.0b013e31818cdbaagoogle scholar: lookup
  18. Nixon AJ, Rickey E, Butler TJ, Scimeca MS, Moran N, Matthews GL. A chondrocyte infiltrated collagen type I/III membrane (MACI® implant) improves cartilage healing in the equine patellofemoral joint model.. Osteoarthritis Cartilage 2015 Apr;23(4):648-60.
    pubmed: 25575968doi: 10.1016/j.joca.2014.12.021google scholar: lookup
  19. Lind M, Larsen A, Clausen C, Osther K, Everland H. Cartilage repair with chondrocytes in fibrin hydrogel and MPEG polylactide scaffold: an in vivo study in goats.. Knee Surg Sports Traumatol Arthrosc 2008 Jul;16(7):690-8.
    pubmed: 18418579doi: 10.1007/s00167-008-0522-1google scholar: lookup
  20. Bekkers JE, Tsuchida AI, van Rijen MH, Vonk LA, Dhert WJ, Creemers LB, Saris DB. Single-stage cell-based cartilage regeneration using a combination of chondrons and mesenchymal stromal cells: comparison with microfracture.. Am J Sports Med 2013 Sep;41(9):2158-66.
    pubmed: 23831891doi: 10.1177/0363546513494181google scholar: lookup
  21. Brehm W, Aklin B, Yamashita T, Rieser F, Trüb T, Jakob RP, Mainil-Varlet P. Repair of superficial osteochondral defects with an autologous scaffold-free cartilage construct in a caprine model: implantation method and short-term results.. Osteoarthritis Cartilage 2006 Dec;14(12):1214-26.
    pubmed: 16820305doi: 10.1016/j.joca.2006.05.002google scholar: lookup
  22. Hale BW, Goodrich LR, Frisbie DD, McIlwraith CW, Kisiday JD. Effect of scaffold dilution on migration of mesenchymal stem cells from fibrin hydrogels.. Am J Vet Res 2012 Feb;73(2):313-8.
    pubmed: 22280396doi: 10.2460/ajvr.73.2.313google scholar: lookup
  23. Goodrich LR, Chen AC, Werpy NM, Williams AA, Kisiday JD, Su AW, Cory E, Morley PS, McIlwraith CW, Sah RL, Chu CR. Addition of Mesenchymal Stem Cells to Autologous Platelet-Enhanced Fibrin Scaffolds in Chondral Defects: Does It Enhance Repair?. J Bone Joint Surg Am 2016 Jan 6;98(1):23-34.
    pmc: PMC4697360pubmed: 26738900doi: 10.2106/jbjs.o.00407google scholar: lookup
  24. Visser J, Melchels FP, Jeon JE, van Bussel EM, Kimpton LS, Byrne HM, Dhert WJ, Dalton PD, Hutmacher DW, Malda J. Reinforcement of hydrogels using three-dimensionally printed microfibres.. Nat Commun 2015 Apr 28;6:6933.
    pubmed: 25917746doi: 10.1038/ncomms7933google scholar: lookup
  25. Frenkel SR, Di Cesare PE. Scaffolds for articular cartilage repair.. Ann Biomed Eng 2004 Jan;32(1):26-34.
    pubmed: 14964719doi: 10.1023/b:abme.0000007788.41804.0dgoogle scholar: lookup
  26. Knecht S, Erggelet C, Endres M, Sittinger M, Kaps C, Stüssi E. Mechanical testing of fixation techniques for scaffold-based tissue-engineered grafts.. J Biomed Mater Res B Appl Biomater 2007 Oct;83(1):50-7.
    pubmed: 17318819doi: 10.1002/jbm.b.30765google scholar: lookup
  27. Thorn JJ, Sørensen H, Weis-Fogh U, Andersen M. Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery.. Int J Oral Maxillofac Surg 2004 Jan;33(1):95-100.
    pubmed: 14690664doi: 10.1054/ijom.2003.0461google scholar: lookup
  28. Cavichiolo JB, Buschle M, Carvalho B. Comparison of fibrin adhesives prepared by 3 different methods.. Int Arch Otorhinolaryngol 2013 Jan;17(1):62-5.
    pmc: PMC4423290pubmed: 25991996doi: 10.7162/s1809-97772013000100011google scholar: lookup
  29. Schuurman W, Khristov V, Pot MW, van Weeren PR, Dhert WJ, Malda J. Bioprinting of hybrid tissue constructs with tailorable mechanical properties.. Biofabrication 2011 Jun;3(2):021001.
    pubmed: 21597163doi: 10.1088/1758-5082/3/2/021001google scholar: lookup
  30. Mouser VH, Abbadessa A, Levato R, Hennink WE, Vermonden T, Gawlitta D, Malda J. Development of a thermosensitive HAMA-containing bio-ink for the fabrication of composite cartilage repair constructs.. Biofabrication 2017 Mar 23;9(1):015026.
    pmc: PMC7116181pubmed: 28229956doi: 10.1088/1758-5090/aa6265google scholar: lookup
  31. Hesse E, Freudenberg U, Niemietz T, Greth C, Weisser M, Hagmann S, Binner M, Werner C, Richter W. Peptide-functionalized starPEG/heparin hydrogels direct mitogenicity, cell morphology and cartilage matrix distribution in vitro and in vivo.. J Tissue Eng Regen Med 2018 Jan;12(1):229-239.
    doi: 10.1002/term.2404pubmed: 28083992google scholar: lookup
  32. Stichler S, Bertlein S, Jüngst T, Böck T, Hesse E, Renz Y, Seebach E, Mancini I, Van Weeren R, Richter W. Thiol-ene cross-linked polyglycidol-hyaluronic acid hybrid hydrogels: preparation, cell loading, 3D printing, and in vivo evaluation.. Front Bioeng Biotechnol Conference abstract: 10th World Biomaterials Congress.
    doi: 10.3389/conf.FBIOE.2016.01.00688google scholar: lookup
  33. Drobnic M, Radosavljevic D, Ravnik D, Pavlovcic V, Hribernik M. Comparison of four techniques for the fixation of a collagen scaffold in the human cadaveric knee.. Osteoarthritis Cartilage 2006 Apr;14(4):337-44.
    pubmed: 16406616doi: 10.1016/j.joca.2005.11.007google scholar: lookup
  34. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage.. Biomaterials 2000 Dec;21(24):2529-43.
    pubmed: 11071603doi: 10.1016/s0142-9612(00)00121-6google scholar: lookup
  35. Groen WM, Diloksumpan P, van Weeren PR, Levato R, Malda J. From intricate to integrated: Biofabrication of articulating joints.. J Orthop Res 2017 Oct;35(10):2089-2097.
    doi: 10.1002/jor.23602pmc: PMC5655743pubmed: 28621834google scholar: lookup
  36. Huey DJ, Hu JC, Athanasiou KA. Unlike bone, cartilage regeneration remains elusive.. Science 2012 Nov 16;338(6109):917-21.
    pmc: PMC4327988pubmed: 23161992doi: 10.1126/science.1222454google scholar: lookup
  37. Cucchiarini M, Madry H, Guilak F, Saris DB, Stoddart MJ, Koon Wong M, Roughley P. A vision on the future of articular cartilage repair.. Eur Cell Mater 2014 May 6;27:12-6.
    pubmed: 24802612doi: 10.22203/ecm.v027sa03google scholar: lookup
  38. Kon E, Filardo G, Di Martino A, Marcacci M. ACI and MACI.. J Knee Surg 2012;25:017.
  39. Marmotti A, Bruzzone M, Bonasia DE, Castoldi F, Von Degerfeld MM, Bignardi C, Mattia S, Maiello A, Rossi R, Peretti GM. Autologous cartilage fragments in a composite scaffold for one stage osteochondral repair in a goat model.. Eur Cell Mater 2013 Aug 4;26:15-31; discussion 31-2.
    pubmed: 23913344doi: 10.22203/ecm.v026a02google scholar: lookup
  40. Nixon AJ, Fortier LA, Williams J, Mohammed H. Enhanced repair of extensive articular defects by insulin-like growth factor-I-laden fibrin composites.. J Orthop Res 1999 Jul;17(4):475-87.
    pubmed: 10459752doi: 10.1002/jor.1100170404google scholar: lookup
  41. Fisher MB, Belkin NS, Milby AH, Henning EA, Bostrom M, Kim M, Pfeifer C, Meloni G, Dodge GR, Burdick JA, Schaer TP, Steinberg DR, Mauck RL. Cartilage repair and subchondral bone remodeling in response to focal lesions in a mini-pig model: implications for tissue engineering.. Tissue Eng Part A 2015 Feb;21(3-4):850-60.
    pmc: PMC4333259pubmed: 25318414doi: 10.1089/ten.tea.2014.0384google scholar: lookup
  42. Vasara AI, Hyttinen MM, Lammi MJ, Lammi PE, Långsjö TK, Lindahl A, Peterson L, Kellomäki M, Konttinen YT, Helminen HJ, Kiviranta I. Subchondral bone reaction associated with chondral defect and attempted cartilage repair in goats.. Calcif Tissue Int 2004 Jan;74(1):107-14.
    pubmed: 14564432doi: 10.1007/s00223-002-2153-8google scholar: lookup
  43. Kold SE, Hickman J, Melsen F. An experimental study of the healing process of equine chondral and osteochondral defects.. Equine Vet J 1986 Jan;18(1):18-24.
    pubmed: 3948824doi: 10.1111/j.2042-3306.1986.tb03529.xgoogle scholar: lookup
  44. White AA 3rd, Panjabi MM, Southwick WO. The four biomechanical stages of fracture repair.. J Bone Joint Surg Am 1977 Mar;59(2):188-92.
    pubmed: 845202
  45. Ahern BJ, Parvizi J, Boston R, Schaer TP. Preclinical animal models in single site cartilage defect testing: a systematic review.. Osteoarthritis Cartilage 2009 Jun;17(6):705-13.
    pubmed: 19101179doi: 10.1016/j.joca.2008.11.008google scholar: lookup
  46. Seegers WH. Blood Clotting Enzymology. New York, London: Academic Press; 2013.
  47. Meade TW, Chakrabarti R, Haines AP, North WR, Stirling Y. Characteristics affecting fibrinolytic activity and plasma fibrinogen concentrations.. Br Med J 1979 Jan 20;1(6157):153-6.
    pmc: PMC1597599pubmed: 420998doi: 10.1136/bmj.1.6157.153google scholar: lookup
  48. Vindas Bolaños RA, Cokelaere SM, Estrada McDermott JM, Benders KE, Gbureck U, Plomp SG, Weinans H, Groll J, van Weeren PR, Malda J. The use of a cartilage decellularized matrix scaffold for the repair of osteochondral defects: the importance of long-term studies in a large animal model.. Osteoarthritis Cartilage 2017 Mar;25(3):413-420.
    pmc: PMC7116104pubmed: 27554995doi: 10.1016/j.joca.2016.08.005google scholar: lookup
  49. Frisbie DD, Oxford JT, Southwood L, Trotter GW, Rodkey WG, Steadman JR, Goodnight JL, McIlwraith CW. Early events in cartilage repair after subchondral bone microfracture.. Clin Orthop Relat Res 2003 Feb;(407):215-27.
    pubmed: 12567150doi: 10.1097/00003086-200302000-00031google scholar: lookup
  50. Cook JL, Hung CT, Kuroki K, Stoker AM, Cook CR, Pfeiffer FM, Sherman SL, Stannard JP. Animal models of cartilage repair.. Bone Joint Res 2014;3(4):89-94.
    pmc: PMC3974069pubmed: 24695750doi: 10.1302/2046-3758.34.2000238google scholar: lookup
  51. Chu CR, Szczodry M, Bruno S. Animal models for cartilage regeneration and repair.. Tissue Eng Part B Rev 2010 Feb;16(1):105-15.
    pmc: PMC3121784pubmed: 19831641doi: 10.1089/ten.teb.2009.0452google scholar: lookup
  52. McCoy AM. Animal Models of Osteoarthritis: Comparisons and Key Considerations.. Vet Pathol 2015 Sep;52(5):803-18.
    pubmed: 26063173doi: 10.1177/0300985815588611google scholar: lookup

Citations

This article has been cited 15 times.
  1. Galarraga JH, Zlotnick HM, Locke RC, Gupta S, Fogarty NL, Masada KM, Stoeckl BD, Laforest L, Castilho M, Malda J, Levato R, Carey JL, Mauck RL, Burdick JA. Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects.. Int J Bioprint 2023;9(5):775.
    doi: 10.18063/ijb.775pubmed: 37457945google scholar: lookup
  2. Du J, Zhu Z, Liu J, Bao X, Wang Q, Shi C, Zhao C, Xu G, Li D. 3D-printed gradient scaffolds for osteochondral defects: Current status and perspectives.. Int J Bioprint 2023;9(4):724.
    doi: 10.18063/ijb.724pubmed: 37323482google scholar: lookup
  3. Bai L, Tao G, Feng M, Xie Y, Cai S, Peng S, Xiao J. Hydrogel Drug Delivery Systems for Bone Regeneration.. Pharmaceutics 2023 Apr 25;15(5).
    doi: 10.3390/pharmaceutics15051334pubmed: 37242576google scholar: lookup
  4. Tampieri A, Kon E, Sandri M, Campodoni E, Dapporto M, Sprio S. Marine-Inspired Approaches as a Smart Tool to Face Osteochondral Regeneration.. Mar Drugs 2023 Mar 28;21(4).
    doi: 10.3390/md21040212pubmed: 37103351google scholar: lookup
  5. Santos-Beato P, Midha S, Pitsillides AA, Miller A, Torii R, Kalaskar DM. Biofabrication of the osteochondral unit and its applications: Current and future directions for 3D bioprinting.. J Tissue Eng 2022 Jan-Dec;13:20417314221133480.
    doi: 10.1177/20417314221133480pubmed: 36386465google scholar: lookup
  6. Tangyuenyong S, Kongdang P, Sirikaew N, Ongchai S. First study on the effect of transforming growth factor beta 1 and insulin-like growth factor 1 on the chondrogenesis of elephant articular chondrocytes in a scaffold-based 3D culture model.. Vet World 2022 Jul;15(7):1869-1879.
    doi: 10.14202/vetworld.2022.1869-1879pubmed: 36185520google scholar: lookup
  7. Browe DC, Burdis R, Díaz-Payno PJ, Freeman FE, Nulty JM, Buckley CT, Brama PAJ, Kelly DJ. Promoting endogenous articular cartilage regeneration using extracellular matrix scaffolds.. Mater Today Bio 2022 Dec;16:100343.
    doi: 10.1016/j.mtbio.2022.100343pubmed: 35865410google scholar: lookup
  8. Zlotnick HM, Locke RC, Hemdev S, Stoeckl BD, Gupta S, Peredo AP, Steinberg DR, Carey JL, Lee D, Dodge GR, Mauck RL. Gravity-based patterning of osteogenic factors to preserve bone structure after osteochondral injury in a large animal model.. Biofabrication 2022 Jul 5;14(4).
    doi: 10.1088/1758-5090/ac79cdpubmed: 35714576google scholar: lookup
  9. Lesage C, Lafont M, Guihard P, Weiss P, Guicheux J, Delplace V. Material-Assisted Strategies for Osteochondral Defect Repair.. Adv Sci (Weinh) 2022 May;9(16):e2200050.
    doi: 10.1002/advs.202200050pubmed: 35322596google scholar: lookup
  10. Doyle SE, Snow F, Duchi S, O'Connell CD, Onofrillo C, Di Bella C, Pirogova E. 3D Printed Multiphasic Scaffolds for Osteochondral Repair: Challenges and Opportunities.. Int J Mol Sci 2021 Nov 17;22(22).
    doi: 10.3390/ijms222212420pubmed: 34830302google scholar: lookup
  11. Lotz B, Bothe F, Deubel AK, Hesse E, Renz Y, Werner C, Schäfer S, Böck T, Groll J, von Rechenberg B, Richter W, Hagmann S. Preclinical Testing of New Hydrogel Materials for Cartilage Repair: Overcoming Fixation Issues in a Large Animal Model.. Int J Biomater 2021;2021:5583815.
    doi: 10.1155/2021/5583815pubmed: 34239571google scholar: lookup
  12. Patel JM, Sennett ML, Martin AR, Saleh KS, Eby MR, Ashley BS, Miller LM, Dodge GR, Burdick JA, Carey JL, Mauck RL. Resorbable Pins to Enhance Scaffold Retention in a Porcine Chondral Defect Model.. Cartilage 2021 Dec;13(2_suppl):1676S-1687S.
    doi: 10.1177/1947603520962568pubmed: 33034511google scholar: lookup
  13. Kilian D, Ahlfeld T, Akkineni AR, Bernhardt A, Gelinsky M, Lode A. 3D Bioprinting of osteochondral tissue substitutes - in vitro-chondrogenesis in multi-layered mineralized constructs.. Sci Rep 2020 May 19;10(1):8277.
    doi: 10.1038/s41598-020-65050-9pubmed: 32427838google scholar: lookup
  14. Lim KS, Abinzano F, Bernal PN, Albillos Sanchez A, Atienza-Roca P, Otto IA, Peiffer QC, Matsusaki M, Woodfield TBF, Malda J, Levato R. One-Step Photoactivation of a Dual-Functionalized Bioink as Cell Carrier and Cartilage-Binding Glue for Chondral Regeneration.. Adv Healthc Mater 2020 Aug;9(15):e1901792.
    doi: 10.1002/adhm.201901792pubmed: 32324342google scholar: lookup
  15. Bothe F, Deubel AK, Hesse E, Lotz B, Groll J, Werner C, Richter W, Hagmann S. Treatment of Focal Cartilage Defects in Minipigs with Zonal Chondrocyte/Mesenchymal Progenitor Cell Constructs.. Int J Mol Sci 2019 Feb 2;20(3).
    doi: 10.3390/ijms20030653pubmed: 30717402google scholar: lookup
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