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Life (Basel, Switzerland)2025; 15(11); 1721; doi: 10.3390/life15111721

Forage Carbohydrate Profiles and Endocrine Morphometric Interactions in Traditionally Managed Horses from Romania.

Abstract: Horses maintained under traditional management systems and dependent on natural forages are often exposed to seasonal and compositional variations that can affect metabolic homeostasis. This study examined associations between forage nutrient composition and metabolic-morphometric indicators in horses from four agroecologically distinct regions of northwestern Romania. Eighty-eight horses managed under semi-extensive rural conditions underwent clinical examination, body condition scoring (BCS), cresty neck scoring (CNS), and fasting blood sampling. Forage samples ( = 34) from daily rations were analyzed for fermentable carbohydrate content, while serum insulin, leptin, and adiponectin were quantified using validated equine-specific ELISA assays. Forage composition varied substantially among regions, influencing both endocrine and morphometric outcomes. Horses consuming carbohydrate-rich forages exhibited higher insulin (0.95-219 μIU/mL) and leptin concentrations (925-28,190 pg/mL), accompanied by elevated BCS and CNS scores, whereas adiponectin levels tended to decrease with increasing carbohydrate content. These findings demonstrate that naturally occurring variation in forage quality can significantly influence metabolic regulation in horses managed under low-input, traditional systems. Integrating forage nutrient evaluation with clinical and endocrine assessments provides a practical framework for identifying animals at risk of metabolic dysfunction and guiding nutritional strategies to mitigate the incidence of laminitis and related disorders.
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Publication Date: 2025-11-06 PubMed ID: 41302145PubMed Central: PMC12653405DOI: 10.3390/life15111721Google Scholar: Lookup
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(2025). Forage Carbohydrate Profiles and Endocrine Morphometric Interactions in Traditionally Managed Horses from Romania. Life (Basel), 15(11), 1721. https://doi.org/10.3390/life15111721

Publication

Life (Basel, Switzerland)
ISSN: 2075-1729
NlmUniqueID: 101580444
Country: Switzerland
Language: English
Volume: 15
Issue: 11
PII: 1721

Researcher Affiliations

Conflict of Interest Statement

The authors declare no conflicts of interest.

References

This article includes 56 references
  1. Patterson-Kane J.C., Karikoski N.P., McGowan C.M. Paradigm Shifts in Understanding Equine Laminitis. Vet. J. 2018;231:33–40. doi: 10.1016/j.tvjl.2017.11.011.
    doi: 10.1016/j.tvjl.2017.11.011pubmed: 29429485google scholar: lookup
  2. Heymering H.W. A Historical Perspective of Laminitis. Vet. Clin. N. Am. Equine Pract. 2010;26:1–11. doi: 10.1016/j.cveq.2009.12.004.
    doi: 10.1016/j.cveq.2009.12.004pubmed: 20381731google scholar: lookup
  3. Manfredi J.M., Stapley E.D., Nadeau J.A., Nash D. Investigation of the Effects of a Dietary Supplement on Insulin and Adipokine Concentrations in Equine Metabolic Syndrome/Insulin Dysregulation. J. Equine Vet. Sci. 2020;88:102930. doi: 10.1016/j.jevs.2020.102930.
    doi: 10.1016/j.jevs.2020.102930pubmed: 32303322google scholar: lookup
  4. Karikoski N.P., McGowan C.M., Singer E.R., Asplin K.E., Tulamo R.M., Patterson-Kane J.C. Pathology of Natural Cases of Equine Endocrinopathic Laminitis Associated with Hyperinsulinemia. Vet. Pathol. 2015;52:945–956. doi: 10.1177/0300985814549212.
    doi: 10.1177/0300985814549212pubmed: 25232034google scholar: lookup
  5. Collins S.N., van Eps A.W., Pollitt C.C., Kuwano A. The Lamellar Wedge. Vet. Clin. N. Am. Equine Pract. 2010;26:179–195. doi: 10.1016/j.cveq.2010.01.004.
    doi: 10.1016/j.cveq.2010.01.004pubmed: 20381746google scholar: lookup
  6. Pollitt C.C. Equine Laminitis. Clin. Tech. Equine Pract. 2004;3:34–44. doi: 10.1053/j.ctep.2004.07.003.
    doi: 10.1053/j.ctep.2004.07.003google scholar: lookup
  7. Zak A., Siwinska N., Elzinga S., Barker V.D., Stefaniak T., Schanbacher B.J., Place N.J., Niedzwiedz A., Adams A.A. Effects of Equine Metabolic Syndrome on Inflammation and Acute-Phase Markers in Horses. Domest. Anim. Endocrinol. 2020;72:106448. doi: 10.1016/j.domaniend.2020.106448.
    doi: 10.1016/j.domaniend.2020.106448pubmed: 32247989google scholar: lookup
  8. Bogaert H., De Koster J., Van den Broeck W., Van Eetvelde M., Opsomer G. Effects of Overconditioning on Pancreatic Insulin Secretory Capacity, Fat Infiltration, and the Number and Size of Islets in Dairy Cows at the End of the Dry Period. J. Dairy Sci. 2018;101:11413–11420. doi: 10.3168/jds.2018-14931.
    doi: 10.3168/jds.2018-14931pubmed: 30316589google scholar: lookup
  9. Shi K., Li R., Xu Z., Zhang Q. Identification of Crucial Genetic Factors, Such as PPARγ, That Regulate the Pathogenesis of Fatty Liver Disease in Dairy Cows Is Imperative for the Sustainable Development of Dairy Industry. Animals. 2020;10:639. doi: 10.3390/ani10040639.
    doi: 10.3390/ani10040639pmc: PMC7222768pubmed: 32272794google scholar: lookup
  10. Carter R.A., McCutcheon L.J., George L.A., Smith T.L., Frank N., Geor R.J. Effects of Diet-Induced Weight Gain on Insulin Sensitivity and Plasma Hormone and Lipid Concentrations in Horses. Am. J. Vet. Res. 2009;70:1250–1258. doi: 10.2460/ajvr.70.10.1250.
    doi: 10.2460/ajvr.70.10.1250pubmed: 19795940google scholar: lookup
  11. Frank N. Equine Metabolic Syndrome. Vet. Clin. N. Am. Equine Pract. 2011;27:73–92. doi: 10.1016/j.cveq.2010.12.004.
    doi: 10.1016/j.cveq.2010.12.004pubmed: 21392655google scholar: lookup
  12. Selim S., Elo K., Jaakkola S., Karikoski N., Boston R., Reilas T., Särkijärvi S., Saastamoinen M., Kokkonen T. Relationships among Body Condition, Insulin Resistance and Subcutaneous Adipose Tissue Gene Expression during the Grazing Season in Mares. PLoS ONE. 2015;10:e0125968. doi: 10.1371/journal.pone.0125968.
    doi: 10.1371/journal.pone.0125968pmc: PMC4418745pubmed: 25938677google scholar: lookup
  13. Sojka-Kritchevsky J.E., Johnson P.J. Current Status and Future Directions: Equine Pituitary Pars Intermedia Dysfunction and Equine Metabolic Syndrome. Equine Vet. J. 2014;46:99–102. doi: 10.1111/evj.12194.
    doi: 10.1111/evj.12194pubmed: 24329586google scholar: lookup
  14. Opsomer G., Wensing T., Laevens H., Coryn M., De Kruif A. Insulin Resistance: The Link between Metabolic Disorders and Cystic Ovarian Disease in High Yielding Dairy Cows? Anim. Reprod. Sci. 1999;56:211–222. doi: 10.1016/S0378-4320(99)00048-2.
    doi: 10.1016/S0378-4320(99)00048-2pubmed: 10497917google scholar: lookup
  15. Patterson Rosa L., Mallicote M.F., Long M.T., Brooks S.A. Metabogenomics Reveals Four Candidate Regions Involved in the Pathophysiology of Equine Metabolic Syndrome. Mol. Cell. Probes. 2020;53:101620. doi: 10.1016/j.mcp.2020.101620.
    doi: 10.1016/j.mcp.2020.101620pmc: PMC7494547pubmed: 32659253google scholar: lookup
  16. de Laat M.A., Sillence M.N., Reiche D.B. Phenotypic, Hormonal, and Clinical Characteristics of Equine Endocrinopathic Laminitis. J. Vet. Intern. Med. 2019;33:1456–1463. doi: 10.1111/jvim.15419.
    doi: 10.1111/jvim.15419pmc: PMC6524085pubmed: 30697823google scholar: lookup
  17. Eades S.C. Overview of Current Laminitis Research. Vet. Clin. Equine Pract. 2010;26:51–63. doi: 10.1016/j.cveq.2010.01.001.
    doi: 10.1016/j.cveq.2010.01.001pubmed: 20381735google scholar: lookup
  18. Loos C.M.M., Dorsch S.C., Elzinga S.E., Brewster-Barnes T., Vanzant E.S., Adams A.A., Urschel K.L. A High Protein Meal Affects Plasma Insulin Concentrations and Amino Acid Metabolism in Horses with Equine Metabolic Syndrome. Vet. J. 2019;251:105341. doi: 10.1016/j.tvjl.2019.105341.
    doi: 10.1016/j.tvjl.2019.105341pubmed: 31492392google scholar: lookup
  19. Meier A.D., de Laat M.A., Reiche D.B., Pollitt C.C., Walsh D.M., McGree J.M., Sillence M.N. The Oral Glucose Test Predicts Laminitis Risk in Ponies Fed a Diet High in Nonstructural Carbohydrates. Domest. Anim. Endocrinol. 2018;63:1–9. doi: 10.1016/j.domaniend.2017.10.008.
    doi: 10.1016/j.domaniend.2017.10.008pubmed: 29172109google scholar: lookup
  20. Treiber K.H., Boston R.C., Kronfeld D.S., Staniar W.B., Harris P.A. Insulin Resistance and Compensation in Thoroughbred Weanlings Adapted to High-Glycemic Meals. J. Anim. Sci. 2005;83:2357–2364. doi: 10.2527/2005.83102357x.
    doi: 10.2527/2005.83102357xpubmed: 16160047google scholar: lookup
  21. Kagan I.A., Kirch B.H., Thatcher C.D., Strickland J.R., Teutsch C.D., Elvinger F., Pleasant R.S. Seasonal and Diurnal Variation in Simple Sugar and Fructan Composition of Orchardgrass Pasture and Hay in the Piedmont Region of the United States. J. Equine Vet. Sci. 2011;31:488–497. doi: 10.1016/j.jevs.2011.03.004.
    doi: 10.1016/j.jevs.2011.03.004google scholar: lookup
  22. Elzinga S.E., Weese J.S., Adams A.A. Comparison of the Fecal Microbiota in Horses with Equine Metabolic Syndrome and Metabolically Normal Controls Fed a Similar All-Forage Diet. J. Equine Vet. Sci. 2016;44:9–16. doi: 10.1016/j.jevs.2016.05.010.
    doi: 10.1016/j.jevs.2016.05.010google scholar: lookup
  23. Heliczer N., Gerber V., Bruckmaier R., Van Der Kolk J.H., De Solis C.N. Cardiovascular Findings in Ponies with Equine Metabolic Syndrome. J. Am. Vet. Med. Assoc. 2017;250:1027–1035. doi: 10.2460/javma.250.9.1027.
    doi: 10.2460/javma.250.9.1027pubmed: 28414603google scholar: lookup
  24. Newsome R.F., Green M.J., Bell N.J., Bollard N.J., Mason C.S., Whay H.R., Huxley J.N. A Prospective Cohort Study of Digital Cushion and Corium Thickness. Part 1: Associations with Body Condition, Lesion Incidence, and Proximity to Calving. J. Dairy Sci. 2017;100:4745–4758. doi: 10.3168/jds.2016-12012.
    doi: 10.3168/jds.2016-12012pubmed: 28434744google scholar: lookup
  25. Longland A.C., Byrd B.M. Pasture Nonstructural Carbohydrates and Equine Laminitis. J. Nutr. 2006;136:2099S–2102S. doi: 10.1093/jn/136.7.2099S.
    doi: 10.1093/jn/136.7.2099Spubmed: 16772510google scholar: lookup
  26. McCue M.E., Geor R.J., Schultz N. Equine Metabolic Syndrome: A Complex Disease Influenced by Genetics and the Environment. J. Equine Vet. Sci. 2015;35:367–375. doi: 10.1016/j.jevs.2015.03.004.
    doi: 10.1016/j.jevs.2015.03.004google scholar: lookup
  27. Siegers E.W., De Ruijter-Villani M., Van Doorn D.A., Stout T.A.E., Roelfsema E. Ultrasonographic Measurements of Localized Fat Accumulation in Shetland Pony Mares Fed a Normal v. a High Energy Diet for 2 Years. Animal. 2018;12:1602–1610. doi: 10.1017/S1751731117003251.
    doi: 10.1017/S1751731117003251pubmed: 29198235google scholar: lookup
  28. Stefaniuk-Szmukier M., Piórkowska K., Ropka-Molik K. Equine Metabolic Syndrome: A Complex Disease Influenced by Multifactorial Genetic Factors. Genes. 2023;14:1544. doi: 10.3390/genes14081544.
    doi: 10.3390/genes14081544pmc: PMC10454496pubmed: 37628596google scholar: lookup
  29. View of Influence of Sustainable Fertilization on Yield and Quality Food of Mountain Grassland. [(accessed on 1 July 2025)]. Available online: https://www.notulaebotanicae.ro/index.php/nbha/article/view/10660/8112.
  30. Bora F.D., Babeș A.C., Călugăr A., Jitea M.I., Hoble A., Filimon R.V., Bunea A., Nicolescu A., Bunea C.I. Unravelling Heavy Metal Dynamics in Soil and Honey: A Case Study from Maramureș Region, Romania. Foods. 2023;12:3577. doi: 10.3390/foods12193577.
    doi: 10.3390/foods12193577pmc: PMC10573013pubmed: 37835231google scholar: lookup
  31. Delarocque J., Reiche D.B., Meier A.D., Warnken T., Feige K., Sillence M.N. Metabolic Profile Distinguishes Laminitis-Susceptible and -Resistant Ponies before and after Feeding a High Sugar Diet. BMC Vet. Res. 2021;17:56. doi: 10.1186/s12917-021-02763-7.
    doi: 10.1186/s12917-021-02763-7pmc: PMC7841998pubmed: 33509165google scholar: lookup
  32. Bora F.D., Bunea A., Pop S.R., Baniță S.I., Duşa D.Ş., Chira A., Bunea C.I. Quantification and Reduction in Heavy Metal Residues in Some Fruits and Vegetables: A Case Study Galați County, Romania. Horticulturae. 2022;8:1034. doi: 10.3390/horticulturae8111034.
    doi: 10.3390/horticulturae8111034google scholar: lookup
  33. Buta E., Borșan I.L., Omotă M., Trif E.B., Bunea C.I., Mocan A., Bora F.D., Rózsa S., Nicolescu A. Comparative Phytoremediation Potential of Eichhornia Crassipes, Lemna Minor, and Pistia Stratiotes in Two Treatment Facilities in Cluj County, Romania. Horticulturae. 2023;9:503. doi: 10.3390/horticulturae9040503.
    doi: 10.3390/horticulturae9040503google scholar: lookup
  34. Faur C.A., Zăhan M., Bunea C.I., Hârșan E., Bora F.D., Bunea A. Antiproliferative and Biochemical Evaluation of Rose Extracts: Impact on Tumor and Normal Skin Cells. Front. Plant Sci. 2024;15:1477243. doi: 10.3389/fpls.2024.1477243.
    doi: 10.3389/fpls.2024.1477243pmc: PMC11557480pubmed: 39539295google scholar: lookup
  35. Filimon R.V., Bunea C.I., Nechita A., Bora F.D., Dunca S.I., Mocan A., Filimon R.M. New Malolactic Bacteria Strains Isolated from Wine Microbiota: Characterization and Technological Properties. Fermentation. 2022;8:31. doi: 10.3390/fermentation8010031.
    doi: 10.3390/fermentation8010031google scholar: lookup
  36. Vlad I.A., Bartha S., Goji G., Tăut I., Rebrean F.A., Burescu L.I.N., Pășcuț C.G., Moțiu P.T., Tunduc A., Bunea C.I., et al. Comprehensive Assessment of Potentially Toxic Element (PTE) Contamination in Honey from a Historically Polluted Agro-Industrial Landscape: Implications for Agricultural Sustainability and Food Safety. Agriculture. 2025;15:1176. doi: 10.3390/agriculture15111176.
    doi: 10.3390/agriculture15111176google scholar: lookup
  37. Garber A., Hastie P., Murray J.A. Factors Influencing Equine Gut Microbiota: Current Knowledge. J. Equine Vet. Sci. 2020;88:102943. doi: 10.1016/j.jevs.2020.102943.
    doi: 10.1016/j.jevs.2020.102943pubmed: 32303307google scholar: lookup
  38. Rodiek A.V., Stull C.L. Glycemic Index of Ten Common Horse Feeds. J. Equine Vet. Sci. 2007;27:205–211. doi: 10.1016/j.jevs.2007.04.002.
    doi: 10.1016/j.jevs.2007.04.002google scholar: lookup
  39. Cash C.M., Fitzgerald D.M., Spence R.J., de Laat M.A. Preliminary Analysis of the FAM174A Gene Suggests It Lacks a Strong Association with Equine Metabolic Syndrome in Ponies. Domest. Anim. Endocrinol. 2020;72:106439. doi: 10.1016/j.domaniend.2020.106439.
    doi: 10.1016/j.domaniend.2020.106439pubmed: 32169753google scholar: lookup
  40. Argo C. Equine Obesity: Beyond the Equine Metabolic Syndrome. Acta Vet. Scand. 2015;57:K2. doi: 10.1186/1751-0147-57-S1-K2.
    doi: 10.1186/1751-0147-57-S1-K2google scholar: lookup
  41. Alieva A.S., Olmastroni E., Reutova O.V., Rotar O.P., Konradi A.O., Shlyakhto E.V., Baragetti A., Grigore L., Pellegatta F., Casula M., et al. Prevalence and Relationship between Metabolic Syndrome and Risk of Cardiovascular Disease: Evidence from Two Population-Based Studies. Atheroscler. Suppl. 2020;42:e41–e48. doi: 10.1016/j.atherosclerosissup.2021.01.008.
    doi: 10.1016/j.atherosclerosissup.2021.01.008pubmed: 33589223google scholar: lookup
  42. Korac B., Kalezic A., Pekovic-Vaughan V., Korac A., Jankovic A. Redox Changes in Obesity, Metabolic Syndrome, and Diabetes. Redox Biol. 2021;42:101887. doi: 10.1016/j.redox.2021.101887.
    doi: 10.1016/j.redox.2021.101887pmc: PMC8113039pubmed: 33579666google scholar: lookup
  43. Silva S.R., Payan-Carreira R., Quaresma M., Guedes C.M., Santos A.S. Relationships between Body Condition Score and Ultrasound Skin-Associated Subcutaneous Fat Depth in Equids. Acta Vet. Scand. 2016;58:37–42. doi: 10.1186/s13028-016-0243-2.
    doi: 10.1186/s13028-016-0243-2pmc: PMC5073852pubmed: 27766985google scholar: lookup
  44. Puppel K., Kuczyńska B. Metabolic Profiles of Cow’s Blood; a Review. J. Sci. Food Agric. 2016;96:4321–4328. doi: 10.1002/jsfa.7779.
    doi: 10.1002/jsfa.7779pubmed: 27129620google scholar: lookup
  45. Reynolds A., Keen J.A., Fordham T., Morgan R.A. Adipose Tissue Dysfunction in Obese Horses with Equine Metabolic Syndrome. Equine Vet. J. 2019;51:760–766. doi: 10.1111/evj.13097.
    doi: 10.1111/evj.13097pmc: PMC6850304pubmed: 30866087google scholar: lookup
  46. Watts K.A. Forage and Pasture Management for Laminitic Horses. Clin. Tech. Equine Pract. 2004;3:88–95. doi: 10.1053/j.ctep.2004.07.009.
    doi: 10.1053/j.ctep.2004.07.009google scholar: lookup
  47. Carter R.A., Geor R.J., Burton Staniar W., Cubitt T.A., Harris P.A. Apparent Adiposity Assessed by Standardised Scoring Systems and Morphometric Measurements in Horses and Ponies. Vet. J. 2009;179:204–210. doi: 10.1016/j.tvjl.2008.02.029.
    doi: 10.1016/j.tvjl.2008.02.029pubmed: 18440844google scholar: lookup
  48. Hammon H.M., Stürmer G., Schneider F., Tuchscherer A., Blum H., Engelhard T., Genzel A., Staufenbiel R., Kanitz W. Performance and Metabolic and Endocrine Changes with Emphasis on Glucose Metabolism in High-Yielding Dairy Cows with High and Low Fat Content in Liver after Calving. J. Dairy Sci. 2009;92:1554–1566. doi: 10.3168/jds.2008-1634.
    doi: 10.3168/jds.2008-1634pubmed: 19307636google scholar: lookup
  49. Sumner-Thomson J.M., Vierck J.L., McNamara J.P. Differential Expression of Genes in Adipose Tissue of First-Lactation Dairy Cattle. J. Dairy Sci. 2011;94:361–369. doi: 10.3168/jds.2010-3447.
    doi: 10.3168/jds.2010-3447pubmed: 21183046google scholar: lookup
  50. Tayyab U., Wilkinson R.G., Charlton G.L., Reynolds C.K., Sinclair L.A. Grass Silage Particle Size When Fed with or without Maize Silage Alters Performance, Reticular PH and Metabolism of Holstein-Friesian Dairy Cows. Animal. 2019;13:524–532. doi: 10.1017/S1751731118001568.
    doi: 10.1017/S1751731118001568pubmed: 29983121google scholar: lookup
  51. Wooldridge A.A., Edwards H.G., Plaisance E.P., Applegate R., Taylor D.R., Taintor J., Zhong Q., Judd R.L. Evaluation of high-molecular weight adiponectin in horses. Am. J. Vet. Res. 2012;73:1230–1240. doi: 10.2460/ajvr.73.8.1230.
    doi: 10.2460/ajvr.73.8.1230pubmed: 22849684google scholar: lookup
  52. Superchi P., Sabbioni A., Beretti V., Vecchi I., Simonini F.V. Hydrolyzable and Fermentable Carbohydrates in North Italian Pastures for Horses. Ital. J. Anim. Sci. 2010;9:352–355. doi: 10.4081/ijas.2010.e66.
    doi: 10.4081/ijas.2010.e66google scholar: lookup
  53. Weckman M.J., Karikoski N.P., Raekallio M.R., Box J.R., Kvist L. Genome-Wide Association Study Suggests Genetic Candidate Loci of Insulin Dysregulation in Finnhorses. Vet. J. 2024;303:106063. doi: 10.1016/j.tvjl.2024.106063.
    doi: 10.1016/j.tvjl.2024.106063pubmed: 38232813google scholar: lookup
  54. Bertin F.R., de Laat M.A. The Diagnosis of Equine Insulin Dysregulation. Equine Vet. J. 2017;49:570–576. doi: 10.1111/evj.12703.
    doi: 10.1111/evj.12703pubmed: 28543410google scholar: lookup
  55. de Laat M.A., McGowan C.M., Sillence M.N., Pollitt C.C. Hyperinsulinemic Laminitis. Vet. Clin. N. Am. Equine Pract. 2010;26:257–264. doi: 10.1016/j.cveq.2010.04.003.
    doi: 10.1016/j.cveq.2010.04.003pubmed: 20699173google scholar: lookup
  56. Bowen M. Management of Equine Metabolic Syndrome. Veterinary. 2019;2019:16–18. doi: 10.12968/eqhe.2019.50.16.
    doi: 10.12968/eqhe.2019.50.16google scholar: lookup

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