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Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet.
Abstract: Insulin dysregulation (ID) is a key risk factor for equine endocrinopathic laminitis, but in many cases ID can only be assessed accurately using dynamic tests. The identification of other biomarkers could provide an alternative or adjunct diagnostic method, to allow early intervention before laminitis develops. The present study characterised the metabolome of ponies with varying degrees of ID using basal and postprandial plasma samples obtained during a previous study, which examined the predictive power of blood insulin levels for the development of laminitis, in ponies fed a high-sugar diet. Samples from 10 pre-laminitic (PL - subsequently developed laminitis) and 10 non-laminitic (NL - did not develop laminitis) ponies were used in a targeted metabolomic assay. Differential concentration and pathway analysis were performed using linear models and global tests. Results: Significant changes in the concentration of six glycerophospholipids (adj. P ≤ 0.024) and a global enrichment of the glucose-alanine cycle (adj. P = 0.048) were found to characterise the response of PL ponies to the high-sugar diet. In contrast, the metabolites showed no significant association with the presence or absence of pituitary pars intermedia dysfunction in all ponies. Conclusions: The present results suggest that ID and laminitis risk are associated with alterations in the glycerophospholipid and glucose metabolism, which may help understand and explain some molecular processes causing or resulting from these conditions. The prognostic value of the identified biomarkers for laminitis remains to be investigated in further metabolomic trials in horses and ponies.
Publication Date: 2021-01-28 PubMed ID: 33509165PubMed Central: PMC7841998DOI: 10.1186/s12917-021-02763-7Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This study investigates distinct metabolic characteristics in ponies which are either susceptible or resistant to laminitis, a painful hoof disease. The research identifies potential biomarkers that could help diagnose insulin dysregulation and laminitis risk early, specifically focusing on changes in certain lipids and glucose metabolism in ponies fed a high-sugar diet.
Study Overview
- The research aims to find alternative or supporting diagnostic methods for detecting insulin dysregulation (ID), which is a major risk factor for equine laminitis, a painful hoof condition in horses.
- It further seeks to identify metabolic differences in ponies that either develop laminitis (pre-laminitic or PL) or do not develop laminitis (non-laminitic or NL) when fed a high-sugar diet.
Methods
- The study utilised basal and postprandial (after a meal) plasma samples from a previous study, which investigated whether blood insulin levels could predict laminitis development in ponies given a high-sugar diet.
- Plasma samples were obtained from 10 PL ponies and 10 NL ponies and underwent a targeted metabolomics assay to characterise the metabolome, the complete set of metabolites in a biological sample.
- The researchers performed differential concentration and pathway analysis using linear models and global tests.
Results and Conclusion
- The researchers identified significant changes in the concentration of six types of glycerophospholipids, a class of lipids, in PL ponies’ response to the high-sugar diet.
- An enrichment, or heightened activity, in glucose-alanine cycle was also found in PL ponies — a metabolic process involving the transfer of energy from muscle tissue to the liver.
- These metabolites revealed no significant association with pituitary pars intermedia dysfunction, a common endocrine disorder in horses, across all ponies.
- The authors conclude that the risk of ID and laminitis are associated with changes in glycerophospholipid and glucose metabolism, shedding light on the origin or consequence of these conditions.
- However, further research needs to be conducted to confirm the predictive value of these identified biomarkers for laminitis.
Cite This Article
APA
Delarocque J, Reiche DB, Meier AD, Warnken T, Feige K, Sillence MN.
(2021).
Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet.
BMC Vet Res, 17(1), 56.
https://doi.org/10.1186/s12917-021-02763-7 Publication
Researcher Affiliations
- Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, 30559, Hannover, Germany. julien.delarocque@tiho-hannover.de.
- Boehringer Ingelheim Vetmedica GmbH, 55216, Ingelheim am Rhein, Germany.
- Biology and Environmental Science School, Queensland University of Technology, Brisbane, Queensland, 4000, Australia.
- Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, 30559, Hannover, Germany.
- Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, 30559, Hannover, Germany.
- Biology and Environmental Science School, Queensland University of Technology, Brisbane, Queensland, 4000, Australia.
MeSH Terms
- Animals
- Blood Glucose / analysis
- Diet / veterinary
- Dietary Carbohydrates / adverse effects
- Disease Resistance
- Disease Susceptibility / metabolism
- Disease Susceptibility / veterinary
- Female
- Foot Diseases / etiology
- Foot Diseases / metabolism
- Foot Diseases / veterinary
- Glycerophospholipids / blood
- Hoof and Claw
- Horse Diseases / etiology
- Horse Diseases / metabolism
- Horses / metabolism
- Insulin / blood
- Male
- Metabolome
- Risk Factors
Conflict of Interest Statement
Dania Reiche is employed by Boehringer Ingelheim Vetmedica GmbH. All other authors have declared that no competing interests exist.
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Citations
This article has been cited 3 times.- Fu Y, He Y, Xiang K, Zhao C, He Z, Qiu M, Hu X, Zhang N. The Role of Rumen Microbiota and Its Metabolites in Subacute Ruminal Acidosis (SARA)-Induced Inflammatory Diseases of Ruminants.. Microorganisms 2022 Jul 25;10(8).
- Guo J, Mu R, Li S, Zhang N, Fu Y, Hu X. Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis.. Genes (Basel) 2021 Dec 16;12(12).
- Leung YH, Kenu00e9z u00c1, Grob AJ, Feige K, Warnken T. Associations of plasma sphingolipid profiles with insulin response during oral glucose testing in Icelandic horses.. J Vet Intern Med 2021 Jul;35(4):2009-2018.
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