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BMC veterinary research2017; 13(1); 106; doi: 10.1186/s12917-017-1003-x

FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule.

Abstract: The synovial membrane lines the luminal side of the joint capsule in synovial joints. It maintains joint homeostasis and plays a crucial role in equine joint pathology. When trauma or inflammation is induced in a joint, the synovial membrane influences progression of joint damage. Equine synovial membrane research is hampered by a lack of markers of fibroblast-like synoviocytes (FLS) to distinguish FLS from other fibroblast-like cells in musculoskeletal connective tissues. The aim of this study is to identify potential FLS markers of the equine synovial membrane using microarray to compare between gene expression in equine synovial membrane and the joint capsule in metacarpophalangeal joints. Results: Microarray analysis of tissues from 6 horses resulted in 1167 up-regulated genes in synovial membrane compared with joint capsule. Pathway analysis resulted in 241 candidate genes. Of these, 15 genes were selected for further confirmation as genes potentially expressed by fibroblast-like synoviocytes. Four genes: FOXO1, PXK, PYCARD and SAMD9L were confirmed in 9 horses by qPCR as differentially expressed in synovial membrane compared to joint capsule. Conclusions: In conclusion, FOXO1, PXK, PYCARD and SAMD9L were confirmed as differentially expressed in synovial membrane compared to joint capsule. These four genes are potential markers of fibroblast-like synoviocytes of the synovial membrane. As these genes are overexpressed in synovial membrane compared to joint capsule, these genes could shed light on synovial membrane physiology and its role in joint disease.
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Publication Date: 2017-04-14 PubMed ID: 28410619PubMed Central: PMC5391632DOI: 10.1186/s12917-017-1003-xGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article explores an equine’s joint health by focusing on the way certain genes are expressed differently in the synovial membrane and in the joint capsule. The studied genes could potentially serve as markers for fibroblast-like synoviocytes, helping further research into the physiology of the synovial membrane and its role in joint diseases.

Purpose of the Study

  • The research was conducted to identify potential markers of fibroblast-like synoviocytes (FLS) in the synovial membrane of equines.
  • A better understanding of these markers could help distinguish FLS from other fibroblast-like cells, paving the way for more comprehensive research on joint health in horses.

Methods Employed

  • Gene expression in the synovial membrane and the joint capsule of the metacarpophalangeal joints were compared using a technique known as microarray.
  • The study utilized tissues from 6 horses during the microarray analysis. An additional 9 horses were then used to confirm the differential gene expression found in the microarray analysis by means of qPCR.

Results of the Research

  • The microarray analysis identified 1167 genes which were up-regulated in the synovial membrane compared with the joint capsule.
  • Out of these, pathway analysis singled out 241 candidate genes.
  • Further testing from this pool of candidate genes highlighted 15 genes for possible expression by fibroblast-like synoviocytes.
  • More specifically, four genes – FOXO1, PXK, PYCARD, and SAMD9L – were confirmed to be differentially expressed in the synovial membrane when compared to the joint capsule.

Conclusions and Implications

  • FOXO1, PXK, PYCARD, and SAMD9L were successfully identified as differentially expressed in the synovial membrane and are potential markers for fibroblast-like synoviocytes.
  • As these genes are over-expressed in the synovial membrane, it implies these genes might be key players in the function and physiology of the synovial membrane and potentially in equine joint diseases.
  • The understanding and identification of these genes could lead to improved diagnostic and therapeutic options for equine joint diseases in the future.

Cite This Article

APA
Thomsen LN, Thomsen PD, Downing A, Talbot R, Berg LC. (2017). FOXO1, PXK, PYCARD and SAMD9L are differentially expressed by fibroblast-like cells in equine synovial membrane compared to joint capsule. BMC Vet Res, 13(1), 106. https://doi.org/10.1186/s12917-017-1003-x

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 106

Researcher Affiliations

Thomsen, Line Nymann
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Hoejbakkegaards alle 5, 2630, Taastrup, Denmark.
Thomsen, Preben Dybdahl
  • Department of Veterinary and Animal Sciences, University of Copenhagen, Groennegaardsvej 7, 1870, Frederiksberg C, Denmark.
Downing, Alison
  • Edinburgh Genomics, Ashworth Laboratories, University of Edinburgh, Edinburgh, EH9 3FL, UK.
Talbot, Richard
  • Edinburgh Genomics, Ashworth Laboratories, University of Edinburgh, Edinburgh, EH9 3FL, UK.
Berg, Lise Charlotte
  • Department of Veterinary Clinical Sciences, University of Copenhagen, Hoejbakkegaards alle 5, 2630, Taastrup, Denmark. lcb@sund.ku.dk.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Fibroblasts / metabolism
  • Gene Expression Regulation
  • Horses / metabolism
  • Joint Capsule / cytology
  • Joint Capsule / metabolism
  • Real-Time Polymerase Chain Reaction
  • Synovial Membrane / cytology
  • Synovial Membrane / metabolism
  • Synoviocytes / metabolism
  • Tissue Array Analysis
  • Up-Regulation

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Citations

This article has been cited 2 times.
  1. Gao J, Liu H, Li L, Guo C, Wang Z, Cheng M, Tan S, Chen L, Shi J, Wu H, Feng C, Yu G, Ding C. Comprehensive proteomic characterization of urethral stricture disease in the Chinese population. Front Mol Biosci 2024;11:1401970.
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  2. Zamith Cunha R, Zannoni A, Salamanca G, De Silva M, Rinnovati R, Gramenzi A, Forni M, Chiocchetti R. Expression of cannabinoid (CB1 and CB2) and cannabinoid-related receptors (TRPV1, GPR55, and PPARα) in the synovial membrane of the horse metacarpophalangeal joint. Front Vet Sci 2023;10:1045030.
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