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Acta veterinaria Scandinavica1994; 35(4); 377-382; doi: 10.1186/BF03548311

Freezability and fertility results with uncentrifuged stallion semen.

Abstract: The first (1 to 3) sperm-rich fractions of the ejaculate were collected from 4 stallions using an open-ended vagina. The volume of the collected fractions was 12 +/- 8 ml with a density of 475 +/- 200 million spermatozoa/ml. Before freezing, the semen was diluted with a skim-milk based extender 1:1 to 1:8 (volume of semen: volume of extender), depending on the initial sperm concentration to achieve a final concentration of 100 million/ml. The total number of spermatozoa in an insemination dose ranged from 0.7 to 1 billion spermatozoa. Within 12 h after ovulation, 48 mares were inseminated in 70 cycles. The total single-cycle pregnancy rate at day 21 was 24%, but varied from 10% to 33% per cycle among the stallions. Sammanlagt 19 ejakulat samlades från 4 hingstar med öppen vagina. Totalvolymen av de 1, 2 eller 3 första spermierika fraktionerna var 12 ± 8 ml och koncentrationen 475 ± 200 milj. spermier/ml. Sperman späddes genast efter uppsamlingen med mjölkbaserad spädningsvätska och djupfrystes. Totalantalet spermier i de 7–9 ml inseminationsdoserna var 0.7 till 1 miljard och koncentrationen 100 milj. spermier/ml. Spädningsgraden varierade mellan 1:1 – 1:8 (sperma : spädningsvätska). Vid en follikelstorlek på 35 mm, kontrollerades stona 2 gånger dagligen både med palpation och ultraljud. Senast 12 timmar efter Ovulationen inseminerades 48 ston under sammanlagt 70 brunster. Dräktighetsresultatet (21 d) var 24% per inseminerad brunst. Mellan hingstarna varierade resultatet från 10% till 33%.
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Publication Date: 1994-01-01 PubMed ID: 7676920PubMed Central: PMC8101403DOI: 10.1186/BF03548311Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the freezing capabilities and potential fertility of semen collected from stallions. The study aims to determine the effectiveness of seminal preservation through freezing and the impact of this method on the subsequent fertility rates.

Stallion Semen Collection

  • The research involved the collection of semen from four stallions.
  • Particularly the first to third sperm-rich fractions of the ejaculate were collected with the use of an open-ended vagina.
  • The volume of the ejaculate amounted to approximately 12ml per horse, with a variance of 8ml, and a sperm density of 475 million spermatozoa/ml with a variance of 200 million spermatozoa/ml.

Semen Preservation

  • Before freezing, the semen was diluted with a skim-milk-based extender.
  • The dilution ratio was between 1:1 and 1:8 (volume of semen : volume of extender) and was subject to the initial sperm concentration.
  • The objective here was to maintain a final sperm concentration of 100 million/ml after dilution.
  • With this method, the overall number of spermatozoa in a single insemination dose varied between 0.7 and 1 billion.

Fertility Rates

  • Following ovulation, an aggregation of 48 mares were inseminated in 70 cycles.
  • The study measured the pregnancy rate at 21 days after insemination.
  • In total, the single-cycle pregnancy rate was recorded to be 24%, with variation among the stallions ranging from 10% to 33%.

This research provides insight into how the freezing and dilution of semen can impact stallion fertility, suggesting a significant variance in fertility rates amongst different stallions following this method. The findings may offer valuable considerations for animal breeding and fertility industries.

Cite This Article

APA
Heiskanen ML, Hilden L, Hyyppä S, Kangasniemi A, Pirhonen A, Mäenpää PH. (1994). Freezability and fertility results with uncentrifuged stallion semen. Acta Vet Scand, 35(4), 377-382. https://doi.org/10.1186/BF03548311

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 35
Issue: 4
Pages: 377-382

Researcher Affiliations

Heiskanen, M L
  • Department of Biochemistry & Biotechnology, University of Kuopio, Ypäjä, Finland.
Hilden, L
    Hyyppä, S
      Kangasniemi, A
        Pirhonen, A
          Mäenpää, P H

            MeSH Terms

            • Animals
            • Cryopreservation / veterinary
            • Female
            • Fertility / physiology
            • Horses / physiology
            • Insemination, Artificial / veterinary
            • Male
            • Pregnancy
            • Semen Preservation / veterinary
            • Sperm Motility
            • Spermatozoa / physiology

            References

            This article includes 20 references
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            Citations

            This article has been cited 1 times.
            1. Katila T, Celebi M, Koskinen E. Effect of timing of frozen semen insemination on pregnancy rate in mares. Acta Vet Scand 1996;37(3):361-5.
              doi: 10.1186/BF03548102pubmed: 8996881google scholar: lookup
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