Freezing stallion semen with the new Cáceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability.

The research shows an improvement in the quality of frozen stallion semen when using a newly developed extender called Caceres. Standard extenders weren’t as effective in preserving sperm quality after thawing as the Caceres extender, which also reduced variability between stallions.

Research Method

• The study involved ejaculates from seven different stallions which were divided and frozen using three different extenders: INRA 96 egg yolk glycerol, Ghent, and Caceres. This was done to have a comparative study and test the effectiveness of each extender.
• Once the samples had been thawed, they were evaluated on several parameters including motility, sperm membrane integrity, early membrane changes, acrosome integrity, and mitochondrial membrane potential. These evaluations were crucial in determining the effectiveness of the extenders in preserving the quality of the sperm post-thaw.

Results of the Study

• According to the results, samples frozen with the Caceres extender consistently showed better results post-thaw, in terms of motility and velocity, membrane integrity, and mitochondrial membrane potential. This means that the semen preserved using the Caceres extender maintained better quality.
• The studies confirmed that the increase in quality ranged from 11-17% in motility, 11-14% increase in membrane integrity, with significant values (p<0.05) indicating that the results were statistically significant and not due to chance.

Conclusion

• The study concluded that the application of the new Caceres extender should be considered in tests used to determine the best extender for the preservation of each stallion’s semen individually.
• This implies that the Caceres extender can be a more effective solution for preserving the sperm quality of stallions across various measures, enhancing their potential usability in reproductive processes post-preservation.