Further characterisation of forms of haemosiderin in iron-overloaded tissues.
Abstract: The biochemical and biophysical properties of isolated haemosiderins have been compared to that of another iron-containing protein, termed prehaemosiderin, which sediments through chaotropic potassium iodide only after 20 h of ultracentrifugation, in contrast to that of haemosiderin which is recovered after 2 h of ultracentrifugation. The iron/protein ratio and iron/phosphate ratio were less that that of the corresponding haemosiderin, while the elemental composition was also reduced in many of the prehaemosiderin samples. Mossbauer spectroscopy and electron diffraction identified the predominant presence of ferrihydrite in prehaemosiderin species even though the secondary haemochromatosis haemosiderin iron cores were essentially goethite-like. The majority of the prehaemosiderins isolated showed the presence of an additional peptide band at 17 kDa in addition to that at 21 kDa. Further Mossbauer studies of haemosiderin isolated from untreated secondary haemochromatosis patients showed that goethite was the predominant form of iron present, thereby indicating that the presence of this form of ferrihydrite was not wholly attributable to chelation therapy.
Publication Date: 1994-10-01 PubMed ID: 7925437DOI: 10.1111/j.1432-1033.1994.00187.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research compares forms of haemosiderin (an iron-containing compound) in tissues overloaded with iron, investigating the differences in their biophysical and biochemical characteristics, including measurements of iron, protein, phosphate and elemental composition.
Objective of the Research
- The study’s primary focus was to establish a thorough comparison between the biochemical and biophysical traits of isolated forms of haemosiderin and prehaemosiderin. These are iron-containing proteins often found in tissues overloaded with iron.
Methods and Procedures
- Analysis was done based on the sedimentation rates of prehaemosiderin and haemosiderin during ultracentrifugation.
- Measurements related to iron/protein ratio and iron/phosphate ratio were taken for both types of proteins.
- The elemental composition of prehaemosiderin samples was studied and compared with those of haemosiderin.
- Tools such as Mossbauer spectroscopy (a method used to determine the properties of iron within a substance) and electron diffraction (used to study the arrangement of atoms) were used to identify the different types of iron present in the proteins.
Research Findings
- The most frequently found iron type in prehaemosiderin species was ferrihydrite, even though haemosiderin iron cores in secondary hemochromatosis were primarily goethite-like.
- Differences were found in the peptide composition, with prehaemosiderin showing an additional band at 17kDa in addition to the one at 21kDa.
- Fresh haemosiderin samples isolated from untreated secondary hemochromatosis patients mostly contained goethite; this indicated that the presence of ferrihydrite wasn’t solely due to the iron-chelation therapy.
Value or Implication of the Research
- These findings help shed light on the nature and types of iron in haemosiderin and prehaemosiderin; this information is critical to understanding how iron overload affects tissues and how this process may be potentially modulated or treated.
Cite This Article
APA
Ward RJ, Ramsey M, Dickson DP, Hunt C, Douglas T, Mann S, Aquad F, Peters TJ, Crichton RR.
(1994).
Further characterisation of forms of haemosiderin in iron-overloaded tissues.
Eur J Biochem, 225(1), 187-194.
https://doi.org/10.1111/j.1432-1033.1994.00187.x Publication
Researcher Affiliations
- Department of Clinical Biochemistry, Kings College School of Medicine and Dentistry, London, England.
MeSH Terms
- Animals
- Ferrous Compounds / pharmacology
- Hemochromatosis / metabolism
- Hemochromatosis / pathology
- Hemosiderin / chemistry
- Hemosiderin / isolation & purification
- Hemosiderin / metabolism
- Horses
- Iron / analysis
- Iron / metabolism
- Iron / pharmacology
- Liver / drug effects
- Liver / metabolism
- Liver / ultrastructure
- Metallocenes
- Microscopy, Electron
- Organometallic Compounds / pharmacology
- Rats
- Reference Values
- Spectroscopy, Mossbauer
Citations
This article has been cited 2 times.- Vali SW, Lindahl PA. Low-temperature Mössbauer spectroscopy of organs from (57)Fe-enriched HFE((-/-)) hemochromatosis mice: an iron-dependent threshold for generating hemosiderin.. J Biol Inorg Chem 2023 Mar;28(2):173-185.
- Miyazaki E, Kato J, Kobune M, Okumura K, Sasaki K, Shintani N, Arosio P, Niitsu Y. Denatured H-ferritin subunit is a major constituent of haemosiderin in the liver of patients with iron overload.. Gut 2002 Mar;50(3):413-9.
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