Further evaluation and validation of a commercially available competitive ELISA (cELISA) for the detection of antibodies specific to equine arteritis virus (EAV).
Abstract: The purpose of this study was to further evaluate and validate two commercially available equine arteritis virus (EAV) competitive ELISAs (original and enhanced cELISAs) using archived equine sera from experimentally inoculated animals and field sera submitted for laboratory diagnosis. First, the original and subsequently enhanced cELISAs were compared with the virus neutralisation test (VNT) using a panel of archived serum samples from experimentally inoculated animals. Then, the enhanced cELISA was compared with the VNT using a large panel of archived serum samples. The total number of equine sera tested was 3255, which included sera against 25 different EAV strains. The study confirmed that the enhanced cELISA was more sensitive than the original cELISA. Based on testing sera from experimentally inoculated animals and field sera, the enhanced cELISA had an estimated sensitivity (98.9 percent and 99.6 percent, respectively) and specificity (98.3 percent and 98.7 percent, respectively). The currently marketed enhanced VMRD EAV antibody cELISA test kit (VMRD Inc., Pullman, Washington, USA) has high sensitivity and specificity relative to the VNT. Based on the findings of this study, the authors would propose that the enhanced cELISA should be considered as an alternative approved method to the VNT for the detection of antibodies to EAV.
British Veterinary Association.
Publication Date: 2016-01-05 PubMed ID: 26733051DOI: 10.1136/vr.103362Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Validation Study
- Antibodies
- Clinical Study
- Comparative Study
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Diseases
- Equine Health
- Equine Viral Arteritis
- Immunology
- Infection
- Infectious Disease
- Laboratory Methods
- Serological Surveys
- Veterinary Medicine
- Veterinary Research
- Virology
- Virus
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study was centered on evaluating the effectiveness of two horse arteritis virus (EAV) commercially available ELISAs (original and enhanced), using specimens taken from horses in a lab setting and those submitted for diagnostic purposes.
Objective and Methodology
- The aim of this research was to validate and further examine two types of EAV competitive ELISAs (original and enhanced).
- The research employed archived horse serum from horses that had been experimentally inoculated, as well as serum that had been submitted for diagnostic testing in a real-world setting.
- The two ELISAs were tested against a virus neutralisation test (VNT), which is a standard method for detecting the presence of specific antibodies to a virus.
- The researchers tested more than 3200 different horse sera, which reacted to 25 EAV strains.
Results
- The findings revealed that the enhanced cELISA was more sensitive than the original, meaning it was more likely to correctly identify the presence of EAV antibodies.
- When testing serum from scientifically inoculated animals and field serum, the enhanced cELISA showed an estimated sensitivity of 98.9 percent and 99.6 percent, respectively.
- The same test demonstrated respective specificities of 98.3 percent and 98.7 percent, which are measures of the test’s accuracy in correctly identifying the absence of EAV antibodies,
- The enhanced VMRD EAV antibody cELISA test kit (made by VMRD Inc., based in Pullman, Washington, USA) was revealed to possess comparatively high sensitivity and specificity against the VNT test.
Conclusion
- Through this study, the authors propose that the enhanced cELISA could be deemed a suitable alternative to the VNT in detecting antibodies to EAV.
- This proposition stems from their findings that the enhanced cELISA is quite sensitive and specific in identifying the presence or absence of EAV antibodies, outdoing the original cELISA and comparing well with the VNT.
Cite This Article
APA
Pfahl K, Chung C, Singleton MD, Shuck KM, Go YY, Zhang J, Campos J, Adams E, Adams DS, Timoney PJ, Balasuriya UB.
(2016).
Further evaluation and validation of a commercially available competitive ELISA (cELISA) for the detection of antibodies specific to equine arteritis virus (EAV).
Vet Rec, 178(4), 95.
https://doi.org/10.1136/vr.103362 Publication
Researcher Affiliations
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA University of Kentucky Veterinary Diagnostic Laboratory, Lexington, KY 40512, USA.
- VMRD (Veterinary Medical Research and Development) Inc., Pullman, WA 99163, USA.
- Department of Biostatistics, University of Kentucky, Lexington, KY 40512, USA.
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA.
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA Virus Research and Testing Group, Division of Drug Discovery Research, Korea Research Institute of Chemical Technology, Daejeon, Korea.
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 South 16th St, Ames, IA 50011, USA.
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA.
- VMRD (Veterinary Medical Research and Development) Inc., Pullman, WA 99163, USA.
- VMRD (Veterinary Medical Research and Development) Inc., Pullman, WA 99163, USA.
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA.
- Maxwell H. Gluck Equine Research, Department of Veterinary Science, University of Kentucky, Lexington, KY 40512, USA.
MeSH Terms
- Animals
- Antibodies, Viral / blood
- Arterivirus Infections / diagnosis
- Arterivirus Infections / veterinary
- Enzyme-Linked Immunosorbent Assay / methods
- Enzyme-Linked Immunosorbent Assay / veterinary
- Equartevirus / immunology
- Horse Diseases / diagnosis
- Horse Diseases / virology
- Horses
- Neutralization Tests / veterinary
- Sensitivity and Specificity
Citations
This article has been cited 2 times.- Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Kokado H, Kondo T. Evaluation of two enzyme-linked immunosorbent assays for the detection of antibodies against equine arteritis virus. J Equine Sci 2018 Dec;29(4):111-115.
- Li L, Li S, Ma H, Akhtar MF, Tan Y, Wang T, Liu W, Khan A, Khan MZ, Wang C. An Overview of Infectious and Non-Infectious Causes of Pregnancy Losses in Equine. Animals (Basel) 2024 Jul 2;14(13).
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