GC-MS characterization of urinary metabolites and changes of ethisterone and testosterone profile after oral administration of danazol in equine.

This research investigates the metabolites resulting from the oral administration of Danazol in horses, and the impact on Ethisterone and Testosterone levels, using Gas Chromatography-Mass Spectrometry (GC-MS). The study also seeks to establish the urinary excretion pattern of Ethisterone, a major metabolite, and Testosterone.

Background of the Research

• Danazol, a synthetic derivative of Ethisterone, is commonly used for palliative treatment of endometriosis. Given its structural similarities to Furazabol and Stanozolol, both of which are anabolic steroids, questions surround its use in performance horses.
• Prior to this research, no study had researched the metabolites of Danazol in horses or its effect on hormone levels. The focus of this paper is to identify urine metabolites and understand the androgenic effects of Danazol’s metabolites on horses.

Methodology

• The study involved administering Danazol orally to two healthy Thoroughbred mares. Urine samples were collected over 12 days post-administration at specific intervals to study the excretion pattern.
• The team used Gas Chromatography-Mass Spectrometry (GC-MS) to identify urinary metabolites of Danazol. Specific models of GC-MS were used to probe the excretion profile of Ethisterone and Testosterone as well.
• Urine samples were extracted using a method described by Masse et al. (1989). After extraction, glucuronide and sulfate forms were hydrolyzed, and the resulting solution was analysed using the GC-MS.

Objective

• The aim of this study is to explore the impacts and metabolites of Danazol in horses, and to determine the effects on Ethisterone and Testosterone levels. Considering the controversial use of anabolic steroids in racehorses, such research can provide valuable insights to ensure the health and fairness of equine sports.