Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions.
Abstract: Equine oviductal epithelial cells (OEC) were transformed with simian virus 40 large T antigen (SV 40 T-ag) to create a cell line for the study of the interaction of equine spermatozoa with oviductal epithelium. One cell line was established based on the expression of the SV 40 T-ag and extended lifespan in culture. Immortalized equine OEC retained the characteristics of differentiated OEC such as the formation of monolayers with characteristic epithelial morphology and cell polarization as well as expression of cytokeratin and equine major histocompatibility complex I. Monolayers of immortalized equine OEC retained their functional competence to bind equine spermatozoa in a dose-dependent manner comparable to that of primary equine OEC cultures. This immortalized cell line of equine OEC provides a uniform, readily available system for sperm-OEC co-cultures, and may be a useful model for the study of sperm-oviduct interactions in the horse.
Publication Date: 2000-03-29 PubMed ID: 10735127DOI: 10.1016/S0093-691X(99)00179-XGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study explains the creation of a stable equine oviductal epithelial cell line, which aids in studying the interaction between horse sperm and the oviductal epithelium. This cell line was established using simian virus 40 large T antigen, retaining the key characteristics of primary equine oviductal epithelial cells, and provides a stable system for co-cultures of sperm and oviductal epithelial cells.
Cell Line Creation
- The researchers transformed equine oviductal epithelial cells (OEC) with simian virus 40 large T antigen (SV 40 T-ag).
- This transformation led to the creation of a cell line established based on the expression of SV 40 T-ag and its extended lifespan in culture. This cell line is expected to provide a consistent, easily available system that aids in research regarding the interaction between equine sperm and oviductal epithelium.
Characteristics of the Transformed Cells
- The immortalized equine OEC retains the characteristics of primary OEC. They form monolayers with typical epithelial morphology and cell polarization, showing they maintained the distinct features of differentiated OEC.
- The cells also express cytokeratin and equine major histocompatibility complex I, further pointing to their retention of characteristics of the primary cells.
- These cells also demonstrate functionally competent binding of equine sperm, which is comparable to that of primary equine OEC cultures. This process is dose-dependent, further indicating the useful nature of this cell line.
Potential Use
- The created cell line provides a uniform, readily accessible system for sperm-OEC co-cultures, which are used to study the interactions between sperm and oviductal epithelial cells in a controlled environment.
- It can potentially serve as a helpful model for understanding and further study of sperm-oviduct interactions in horses. Such studies could be crucial in equine reproduction research and might have implications in understanding and treating infertility issues in horses.
Cite This Article
APA
Dobrinski I, Jacob JR, Tennant BC, Ball BA.
(2000).
Generation of an equine oviductal epithelial cell line for the study of sperm-oviduct interactions.
Theriogenology, 52(5), 875-885.
https://doi.org/10.1016/S0093-691X(99)00179-X Publication
Researcher Affiliations
- Center for Animal Transgenesis and Germ Cell Research, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348, USA.
MeSH Terms
- Animals
- Antigens, Polyomavirus Transforming / genetics
- Cell Line, Transformed
- Cell Polarity
- Cells, Cultured
- Epithelial Cells / cytology
- Epithelial Cells / physiology
- Fallopian Tubes / cytology
- Fallopian Tubes / physiology
- Female
- Histocompatibility Antigens Class I / analysis
- Horses
- Keratins / analysis
- Male
- Simian virus 40 / genetics
- Spermatozoa / cytology
- Spermatozoa / physiology
Citations
This article has been cited 1 times.- Leemans B, Bromfield EG, Stout TAE, Vos M, Van Der Ham H, Van Beek R, Van Soom A, Gadella BM, Henning H. Developing a reproducible protocol for culturing functional confluent monolayers of differentiated equine oviduct epithelial cells†.. Biol Reprod 2022 Apr 26;106(4):710-729.
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