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Cell transplantation2016; 25(11); 2003-2016; doi: 10.3727/096368916X691691

Generation of Equine-Induced Pluripotent Stem Cells and Analysis of Their Therapeutic Potential for Muscle Injuries.

Abstract: Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/ mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.
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Publication Date: 2016-05-27 PubMed ID: 27226077DOI: 10.3727/096368916X691691Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper details how a specific type of stem cell derived from horses (equine-induced pluripotent stem cells, or E-iPSCs) was generated and its potential to regenerate damaged muscle tissues in mice, thus suggesting a possible treatment method for muscle injuries in horses.

Equine-Induced Pluripotent Stem Cells Generation

  • The researchers created equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs). The E-ADSCs were transformed into iPSCs using a polycistronic lentiviral vector.
  • This vector contained four specific genes (transcription factors) responsible for the transformation process – Oct4, Sox2, Klf4, and c-Myc.
  • After the transformation, the researchers examined the pluripotent characteristics of the newly generated E-iPSCs, confirming their potential to differentiate into various types of cells.

Equine-Induced Pluripotent Stem Cells and Muscle Regeneration

  • Following the successful generation of E-iPSCs, the cells were then transplanted into mice with muscle injuries (Rag/mdx mice).
  • The histopathological examination post-transplant revealed that the mice exhibited enhanced muscle regeneration compared to the control group. This result indicates an effective response of the injured muscles to E-iPSCs treatment.
  • Additionally, researchers observed that E-iPSC-derived myofibers were present in the injured muscles, suggesting that E-iPSCs are capable of inducing muscle regeneration.

Conclusion

  • The findings from this study demonstrate that it is possible to generate equine-induced pluripotent stem cells from horse fat-derived cells.
  • More importantly, these E-iPSCs have the potential to trigger the regeneration of damaged muscle tissue.
  • This suggests that E-iPSCs could offer a new avenue for therapies aimed at treating muscle injuries in horses and probably in other animals or potentially humans in the future.

Cite This Article

APA
Lee EM, Kim AY, Lee EJ, Park JK, Park SI, Cho SG, Kim HK, Kim SY, Jeong KS. (2016). Generation of Equine-Induced Pluripotent Stem Cells and Analysis of Their Therapeutic Potential for Muscle Injuries. Cell Transplant, 25(11), 2003-2016. https://doi.org/10.3727/096368916X691691

Publication

Cell transplantation
ISSN: 1555-3892
NlmUniqueID: 9208854
Country: United States
Language: English
Volume: 25
Issue: 11
Pages: 2003-2016

Researcher Affiliations

Lee, Eun-Mi
  • College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
  • Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
Kim, Ah-Young
  • College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
  • Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
Lee, Eun-Joo
  • College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
  • Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
Park, Jin-Kyu
  • College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
Park, Se-Il
  • Cardiovascular Product Evaluation Center, College of Medicine, Yonsei University, Seoul, Republic of Korea.
Cho, Ssang-Goo
  • Department of Animal Biotechnology, Animal Resources Research Center, Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Seoul, Republic of Korea.
Kim, Hong Kyun
  • Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Kim, Shin-Yoon
  • Skeletal Diseases Genome Research Center, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
Jeong, Kyu-Shik
  • College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
  • Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.

MeSH Terms

  • Adipose Tissue / cytology
  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Elapid Venoms / pharmacology
  • Embryoid Bodies / metabolism
  • Embryoid Bodies / pathology
  • Gene Expression / drug effects
  • Horses
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism
  • Induced Pluripotent Stem Cells / transplantation
  • Kruppel-Like Factor 4
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred mdx
  • Mice, Transgenic
  • Muscle, Skeletal / pathology
  • Muscle, Skeletal / physiology
  • Muscular Dystrophies / pathology
  • Muscular Dystrophies / therapy
  • Myogenin / genetics
  • Myogenin / metabolism
  • PAX7 Transcription Factor / genetics
  • PAX7 Transcription Factor / metabolism
  • Regeneration
  • Stem Cells / cytology
  • Stem Cells / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Citations

This article has been cited 19 times.
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