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Stem cell research & therapy2020; 11(1); 247; doi: 10.1186/s13287-020-01716-5

Generation of induced pluripotent stem cells from large domestic animals.

Abstract: Induced pluripotent stem cells (iPSCs) have enormous potential in developmental biology studies and in cellular therapies. Although extensively studied and characterized in human and murine models, iPSCs from animals other than mice lack reproducible results. Herein, we describe the generation of robust iPSCs from equine and bovine cells through lentiviral transduction of murine or human transcription factors Oct4, Sox2, Klf4, and c-Myc and from human and murine cells using similar protocols, even when different supplementations were used. The iPSCs were analyzed regarding morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection, and spontaneous and induced differentiation. Although embryonic-derived stem cells are yet not well characterized in domestic animals, generation of iPS cells from these species is possible through similar protocols used for mouse or human cells, enabling the use of pluripotent cells from large animals for basic or applied purposes. Herein, we also infer that bovine iPS (biPSCs) exhibit similarity to mouse iPSCs (miPSCs), whereas equine iPSs (eiPSCs) to human (hiPSCs). The generation of reproducible protocols in different animal species will provide an informative tool for producing in vitro autologous pluripotent cells from domestic animals. These cells will create new opportunities in animal breeding through transgenic technology and will support a new era of translational medicine with large animal models.
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Publication Date: 2020-06-25 PubMed ID: 32586372PubMed Central: PMC7318412DOI: 10.1186/s13287-020-01716-5Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the creation of induced pluripotent stem cells (iPSCs) from large domestic animals such as horses and cows, which has important implications for developmental biology, cellular therapies, animal breeding and translational medicine.

Research Aim and Methodology

  • The study aims to generate induced pluripotent stem cells (iPSCs) from large domestic animals such as horses and cows. These iPSCs have significant potential for developing biology studies and cellular therapies.
  • Researchers generated iPSCs through lentiviral transduction, a process that uses a modified virus to add four specific genes – Oct4, Sox2, Klf4, and c-Myc – to the cells of these animals.
  • The methodology applied here follows similar protocols used to generate iPSCs from mouse and human cells. This is important because previous attempts to create iPSCs from non-murine animals lacked reproducible results.

Findings and Analysis

  • The produced iPSCs were examined for various factors to confirm pluripotency. These include their morphology, gene and protein expression of pluripotency factors, alkaline phosphatase detection (an enzyme used as a marker of pluripotency), and their ability to differentiate spontaneously and upon induction into different cell types.
  • The results showed that the protocols are effective and can be consistently reproduced. This opens up possibilities of generating pluripotent cells from other large domestic animals.

Implication of the Study

  • The researchers hypothesize that iPSCs created from cows are similar to those from mice, while those from horses are closer to human iPSCs. Further research on this hypothesis could provide valuable insights into the nature of iPSCs and their potential applicability across species.
  • By generating iPSCs from large domestic animals, this technology could advance animal breeding via transgenic technology. It could also play a significant role in developing novel cellular therapies for various diseases.
  • The research significantly contributes to translational medicine as large animal models could help study and validate new therapies, which could ultimately be translated to human treatments.

Conclusion

  • This study has established protocols for the generation of iPSCs from large domestic animals, bridging a notable gap in the field of stem cell research. This opens up new possibilities for alternates to embryonic-derived stem cells, which are not clearly characterized in domestic animals.
  • The ability to produce pluripotent cells from large animals for basic or applied purposes will create new opportunities in animal breeding and cellular therapies.

Cite This Article

APA
Bressan FF, Bassanezze V, de Figueiredo Pessôa LV, Sacramento CB, Malta TM, Kashima S, Fantinato Neto P, Strefezzi RF, Pieri NCG, Krieger JE, Covas DT, Meirelles FV. (2020). Generation of induced pluripotent stem cells from large domestic animals. Stem Cell Res Ther, 11(1), 247. https://doi.org/10.1186/s13287-020-01716-5

Publication

Stem cell research & therapy
ISSN: 1757-6512
NlmUniqueID: 101527581
Country: England
Language: English
Volume: 11
Issue: 1
Pages: 247
PII: 247

Researcher Affiliations

Bressan, Fabiana Fernandes
  • Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, Brazil. fabianabressan@usp.br.
  • Postgraduate Program in Anatomy of Domestic and Wild Animals, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. fabianabressan@usp.br.
  • Center for Cell-Based Therapy, Regional Blood Center, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. fabianabressan@usp.br.
Bassanezze, Vinícius
  • Heart Institute (INCOR), Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
  • Present Address: Brigham and Women's Hospital, Harvard Medical School, Boston, USA.
de Figueiredo Pessôa, Laís Vicari
  • Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, Brazil.
  • Postgraduate Program in Anatomy of Domestic and Wild Animals, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Sacramento, Chester Bittencourt
  • Heart Institute (INCOR), Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
  • Present Address: Weill Cornell Medicine, Cornell University, Ithaca, USA.
Malta, Tathiane Maistro
  • Center for Cell-Based Therapy, Regional Blood Center, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
  • School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Kashima, Simone
  • Center for Cell-Based Therapy, Regional Blood Center, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Fantinato Neto, Paulo
  • Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, Brazil.
  • Postgraduate Program in Anatomy of Domestic and Wild Animals, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Strefezzi, Ricardo De Francisco
  • Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Pieri, Naira Caroline Godoy
  • Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, Brazil.
Krieger, José Eduardo
  • Heart Institute (INCOR), Faculty of Medicine, University of São Paulo, São Paulo, Brazil.
Covas, Dimas Tadeu
  • Center for Cell-Based Therapy, Regional Blood Center, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Meirelles, Flávio Vieira
  • Department of Veterinary Medicine, Faculty of Animal Sciences and Food Engineering, University of São Paulo, Pirassununga, Brazil.
  • Postgraduate Program in Anatomy of Domestic and Wild Animals, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
  • Center for Cell-Based Therapy, Regional Blood Center, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.

MeSH Terms

  • Animals
  • Animals, Domestic
  • Cattle
  • Cell Differentiation
  • Cellular Reprogramming
  • Embryonic Stem Cells
  • Fibroblasts
  • Horses
  • Induced Pluripotent Stem Cells
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors / genetics
  • Mice
  • Octamer Transcription Factor-3 / genetics
  • SOXB1 Transcription Factors / genetics

Conflict of Interest Statement

The authors declare that they have no competing interests.

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