Generation of packaging-defective DNA molecules of equine adenovirus.
Abstract: Equine adenovirus (EAd) DNA prepared from infected bovine kidney (MDBK) cells contained additional sequences of about 100 to 700 bp at the left-hand end of the genome. These aberrant viral genomes were produced even after the first passage of the wild type EAd in MDBK cells and their relative amounts did not change significantly during serial passage. The left terminal fragments of two defective viral DNAs were cloned into the plasmid vector pBR322 and the nucleotide sequences of their terminal regions were analyzed. The data indicate that one viral DNA contained a duplication of the inverted terminal repetition (ITR) and the other contained 270 bp of additional sequences derived from the right-terminal region of EAd genome added to the left-terminal, ITR. While the former DNA was packaged into virions, the latter was not, presumable due to the alteration of the distance from the left terminus to the putative DNA packaging signal, reported to be located between 290 and 390 bp (Hammarskjold and Winberg, 1980). The possible mechanism for the generation of these defective DNAs is discussed.
Publication Date: 1986-05-01 PubMed ID: 3008433DOI: 10.1016/0042-6822(86)90104-2Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates how the alteration in the DNA of equine adenovirus results in the generation of defective viral particles, focusing on the changes found at the left end of the viral genome after infecting bovine kidney cells.
Abnormal Viral Genomes
- The study begins by documenting how the DNA of the Equine adenovirus (EAd), prepared from infected bovine kidney cells, showed additional sequences at the left end of the genome.
- The range for these extra sequences was from 100 to 700 base pairs (bp).
- The research also highlights that these modified viral genomes were produced right from the initial infection of the MDBK cells with EAd.
- It is noted that there was no significant change in the amount of these abnormal viral genomes during successive periods of serial passage in the experiment.
Analysis of Abnormalities
- The researchers cloned fragments from the terminal parts of two defective viral DNAs into a plasmid referred to as pBR322 so as to investigate these abnormalities further.
- An exploration of their nucleotide sequences indicated that one viral DNA had a duplicated inverted terminal repetition (ITR) while the other had 270bp of additional sequences.
- The extra 270 bp were identified as being derived from the genome’s right-terminal region, added to the left-terminal ITR of the EAd.
Effects of Abnormality on Viral Function
- The study points out that the DNA with the duplication was packaged into virions (virus particles outside a host cell) due to its structural integrity.
- On the other hand, the other viral DNA with the 270 bp extra sequences was not integrated into virions possibly due to the alteration of the distance from the left terminus (end) to the supposed DNA packaging signal—an alteration that disrupts proper packaging.
DNA Packaging Signal
- This signal is a specific portion of the viral genome recognized by the virus during the assembly of new virus particles.
- Previous research suggests that this packaging signal is located between 290 and 390 bp from the left terminus.
- Disrupting the distance to this signal thereby prevents proper virus assembly.
Speculation on Mechanism of Formation
- The research concludes by proposing possible mechanisms for the generation of these abnormal or defective DNAs, although this is not explicitly detailed in the abstract.
Cite This Article
APA
Ishiyama T, Shinagawa M, Sato G, Fujinaga K, Padmanabhan R.
(1986).
Generation of packaging-defective DNA molecules of equine adenovirus.
Virology, 151(1), 66-76.
https://doi.org/10.1016/0042-6822(86)90104-2 Publication
Researcher Affiliations
MeSH Terms
- Adenoviridae / genetics
- Adenoviridae / growth & development
- Adenoviridae / metabolism
- Animals
- Base Sequence
- Cattle
- Cell Line
- Cloning, Molecular
- DNA Restriction Enzymes
- DNA, Viral / genetics
- DNA, Viral / metabolism
- Deoxyribonuclease BamHI
- Dogs
- Genes, Viral
- Horses
- Kidney
- Repetitive Sequences, Nucleic Acid
- Virion / metabolism
Grant Funding
- CA 33099 / NCI NIH HHS
Citations
This article has been cited 2 times.- Cheng AC, Wang MS, Chen XY, Guo YF, Liu ZY, Fang PF. Pathogenic and pathological characteristic of new type gosling viral enteritis first observed in China.. World J Gastroenterol 2001 Oct;7(5):678-84.
- Liu YC, Abouhaidar MG, Sira S, Campbell JB. Characterization of the genome of a vaccine strain of canine adenovirus type 1.. Virus Genes 1988 Oct;2(1):69-81.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
