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Home / Equine Research Bank / Vet Parasitol / Genetic characterization of Cryptosporidium in animal and hu...
Veterinary parasitology2016; 228; 116-120; doi: 10.1016/j.vetpar.2016.08.015

Genetic characterization of Cryptosporidium in animal and human isolates from Jordan.

Abstract: Little is known about the epidemiology of Cryptosporidium in Jordan and to date, only one genotyping study has been conducted on Cryptosporidium isolates from Jordanian children. In the present study, a total of 284 faecal samples from Jordanian cattle, sheep, goats and chicken and 48 human faecal samples were screened for the presence of Cryptosporidium using an 18S quantitative PCR (qPCR) and a C. parvum/C. hominis specific qPCR at a lectin locus. Of these, 37 of 284 animal faecal samples were positive by qPCR at the 18S locus giving an overall prevalence of 11.6%. The point prevalence of Cryptosporidium in chickens, sheep, horses, cattle and goats ranged from 4.8% (chickens) to 18.7% (cattle). A total of six species were detected; C. xiaoi (n=9),C. andersoni (n=7),C. ryanae (n=5),C. parvum (n=4),C. baileyi (n=1) and a genetically distinct and potentially novel species in two isolates from horses. Sub-genotype analysis of the 4 C. parvum isolates at the 60-kDa glycoprotein (gp60) locus identified subtype IIaA19G2R1 (n=2) and IIaA16GR1 (n=2). For the human samples, 4 positives (8.3% prevalence) were detected. Of these, two were C. parvum (subtypes IIdA20G1 and IIaA15G2R1) and two were C. hominis (subtypes 1bA9G3 and 1bA10G2R2). Further studies are required to better understand the epidemiology and transmission of Cryptosporidium in Jordan.
Copyright © 2016 Elsevier B.V. All rights reserved.
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Publication Date: 2016-08-21 PubMed ID: 27692311DOI: 10.1016/j.vetpar.2016.08.015Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper conducts a detailed study on the prevalence, genetic characterization, and epidemiology of a parasite, Cryptosporidium, in animals and humans in Jordan. This study screened fecal samples from various animals and humans for the presence of Cryptosporidium and provides insights into the types and subtypes of Cryptosporidium species prevalent in Jordan.

Research Aim and Scope

  • The objective of the research was to explore the prevalence and genetic makeup of the Cryptosporidium parasite in Jordan where such epidemiological data is lacking.
  • The study involved screening of 284 animal fecal samples from cattle, sheep, goats, chickens, and horses, and 48 human fecal samples.
  • The samples were examined for the presence of Cryptosporidium using the 18S quantitative PCR (Polymerase Chain Reaction) and a C. parvum/C. hominis specific PCR.

Findings

  • Out of 284 animal fecal samples, 37 were positive for Cryptosporidium, resulting in an overall prevalence rate of 11.6%. The prevalence in different animals ranged from 4.8% in chickens to 18.7% in cattle.
  • Six different species of Cryptosporidium, including a potentially new species found in horses, were detected in animal samples.
  • Four of the 48 human samples were positive for Cryptosporidium, with a prevalence rate of 8.3%. Two samples each were found to be C. parvum and C. hominis.
  • Sub-genotype analysis was further used to identify the subtypes present in the positive samples.

Implications and Conclusions

  • These findings contribute to the broader understanding of the prevalence and genetic diversity of Cryptosporidium in Jordan.
  • The presence of the parasite in both animals and humans indicates possible zoonotic transmission. However, more studies would be required to understand the modes and routes of transmission fully.
  • Identifying the species and subtypes can enlighten the possible disease severity and treatment methods since various Cryptosporidium species affect hosts differently.
  • It also underlines the need for further studies to explore the epidemiology of Cryptosporidium in Jordan and create effective control and prevention strategies.

Cite This Article

APA
Hijjawi N, Mukbel R, Yang R, Ryan U. (2016). Genetic characterization of Cryptosporidium in animal and human isolates from Jordan. Vet Parasitol, 228, 116-120. https://doi.org/10.1016/j.vetpar.2016.08.015

Publication

Veterinary parasitology
ISSN: 1873-2550
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 228
Pages: 116-120
PII: S0304-4017(16)30347-8

Researcher Affiliations

Hijjawi, Nawal
  • Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, The Hashemite University, PO Box 150459, Zarqa, 13115, Jordan.
Mukbel, Rami
  • Department of Basic Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: rmmukbel@just.edu.jo.
Yang, Rongchang
  • Division of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia.
Ryan, Una
  • Division of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia. Electronic address: Una.Ryan@murdoch.edu.au.

MeSH Terms

  • Animals
  • Cattle
  • Cattle Diseases / epidemiology
  • Cattle Diseases / parasitology
  • Chickens
  • Cryptosporidiosis / epidemiology
  • Cryptosporidiosis / parasitology
  • Cryptosporidium / genetics
  • Cryptosporidium / isolation & purification
  • Feces / parasitology
  • Genotype
  • Goat Diseases / epidemiology
  • Goat Diseases / parasitology
  • Goats
  • Horse Diseases / epidemiology
  • Horse Diseases / parasitology
  • Horses
  • Humans
  • Jordan / epidemiology
  • Molecular Typing
  • Poultry Diseases / epidemiology
  • Poultry Diseases / parasitology
  • Prevalence
  • Sheep
  • Zoonoses

Citations

This article has been cited 29 times.
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