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Home / Equine Research Bank / Helminthologia / Genetic Characterization of Hydatid Cysts of Different Inter...
Helminthologia2020; 57(3); 185-195; doi: 10.2478/helm-2020-0031

Genetic Characterization of Hydatid Cysts of Different Intermediate Hosts.

Abstract: Cystic echinococcosis is an important cosmopolitan parasitic zoonosis that causes public health and economic problems in Egypt. The present study was undertaken to identify genotypes of hydatid cyst (HC) DNA isolated from different animal isolates and to identify the genotype of secondary hydatid cysts (HCs) developed in rabbits experimentally infected with camel HC for detection of any genetic mutation. In the present study, we extracted DNA from the germinal layers of 8 HCs collected from 3 camels, 1 cattle, 1 sheep and 3 donkeys in addition to 3 secondary HCs collected from rabbits experimentally infected with camel HC. PCR amplification of the ITS1 gene of all examined samples showed an amplified DNA band at 1115 bp. The partial nucleotide sequences of the ITS1 gene of all isolates were aligned and compared with the reference sequences of the genotypes G1-G8 in GenBank. The camel and rabbit samples were identified as genotype 6 (G6), while the cattle and sheep samples belonged to stricto (G1). The donkey isolates belonged to (G4). Alignment of the ITS1 partial nucleotide sequences of the camel HCs and rabbit secondary HCs isolates with the G6 partial nucleotide sequence in GenBank was performed. Both camel HCs and rabbit secondary HCs isolates exhibited the same sequence identity matrix, which indicated the absence of mutation in the rabbit secondary HCs. It can be concluded that camel and rabbit samples were identified as (G6), the cattle and sheep samples belonged to (G1) and donkey isolates belonged to (G4). No mutation occurred during HCs transmission from camel to rabbit.
© 2020 W. M. Mousa, A. M. Abdel-Wahab, M. El-Gameel Sohila, O. A. Mahdy, published by Sciendo.
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Publication Date: 2020-08-05 PubMed ID: 32855606PubMed Central: PMC7425234DOI: 10.2478/helm-2020-0031Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the genetic types of hydatid cysts, a form of parasitic disease, found in different animal species in Egypt and analysed if the cysts underwent genetic mutation when transferred from camels to rabbits.

Introduction and Methodology

  • This study focused on cystic echinococcosis, a widespread parasitic disease causing health and economic issues in Egypt.
  • It sought to categorize different genotypes of hydatid cyst (HC) DNA drawn from various animal hosts, and explore if secondary hydatid cysts developed in rabbits experimentally infected with camel HC DNA undergo any genetic mutations.
  • The research involved DNA extraction from 8 hydatid cysts sourced from different animals including camels, cattle, sheep, donkeys, and rabbits experimentally infected with camel HC.
  • PCR amplification of the ITS1 gene, a method that allows DNA sequence amplification, was applied to all collected samples.

Results Analysis

  • The amplified ITS1 gene exhibited an amplified DNA band at 1115 base pairs (bp) in every examined sample.
  • Partial sequencing of the ITS1 gene of all isolates was compared to reference sequences of genotypes G1-G8 available in GenBank, a genetic sequence database.
  • By this comparison, genotypes for cysts from different animals were identified: camel and rabbit samples were classified as genotype 6 (G6), cattle and sheep belonged to stricto (G1), and donkey isolates classified into (G4).
  • The research employed alignment of nucleotide sequences for camel HCs and rabbit secondary HCs with the G6 sequence in GenBank for further analysis.

Conclusions

  • The study confirmed again that camel and rabbit samples were found to be of the (G6) genotype, cattle and sheep isolates belonged to (G1), and donkey isolates were of the (G4) type.
  • Significantly, the identity matrix sequence, which compares sequences in detail, was found to be the same for both the camel HCs and rabbit secondary HCs. Therefore, the research established no mutation occurring when HCs are transferred from camels to rabbits.

Cite This Article

APA
Mousa WM, Abdel-Wahab AM, El-Gameel Sohila M, Mahdy OA. (2020). Genetic Characterization of Hydatid Cysts of Different Intermediate Hosts. Helminthologia, 57(3), 185-195. https://doi.org/10.2478/helm-2020-0031

Publication

Helminthologia
ISSN: 0440-6605
NlmUniqueID: 0043764
Country: Germany
Language: English
Volume: 57
Issue: 3
Pages: 185-195

Researcher Affiliations

Mousa, W M
  • Parasitology Department, Faculty of Veterinary Medicine, Cairo University, P.O. 12211 Giza, Egypt.
Abdel-Wahab, A M
  • Parasitology Department, Faculty of Veterinary Medicine, Cairo University, P.O. 12211 Giza, Egypt.
El-Gameel Sohila, M
  • Parasitology Department, Faculty of Veterinary Medicine, Cairo University, P.O. 12211 Giza, Egypt.
Mahdy, O A
  • Parasitology Department, Faculty of Veterinary Medicine, Cairo University, P.O. 12211 Giza, Egypt.

Conflict of Interest Statement

Conflict of Interest Authors state no conflict of interest.

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Citations

This article has been cited 5 times.
  1. Mulinge E, Zeyhle E, Mbae C, Gitau L, Kaburu T, Magambo J, Mackenstedt U, Romig T, Kern P, Wassermann M. Cystic echinococcosis in donkeys in eastern Africa. Parasitology 2023 Apr;150(5):468-475.
    doi: 10.1017/S0031182023000173pubmed: 36843475google scholar: lookup
  2. Elshahawy IS, El-Seify MA, Ahamed ZK, Fawaz MM. Occurrence and Phylogenetic Description of Cystic Echinococcosis Isolate from Egyptian Camel (Camelus Dromedarius). Helminthologia 2022 Sep;59(3):253-264.
    doi: 10.2478/helm-2022-0026pubmed: 36694828google scholar: lookup
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  5. Attia MM, Ibrahim AM, Sakr OG, Salem HM, Barsoum SS, Kamel M, El Gameel SM. Health status of rabbits infested with sarcoptic mange with different lesion scores. J Parasit Dis 2025 Jun;49(2):334-342.
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