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Journal of genetics2005; 84(3); 295-301; doi: 10.1007/BF02715799

Genetic diversity and bottleneck studies in the Marwari horse breed.

Abstract: Genetic diversity within the Marwari breed of horses was evaluated using 26 different microsatellite pairs with 48 DNA samples from unrelated horses. This molecular characterisation was undertaken to evaluate the problem of genetic bottlenecks also, if any, in this breed. The estimated mean (-/+ s.e.) allelic diversity was 5.9 (-/+ 2.24), with a total of 133 alleles. A high level of genetic variability within this breed was observed in terms of high values of mean (-/+ s.e.) effective number of alleles (3.3 -/+ 1.27), observed heterozygosity (0.5306 -/+ 0.22), expected Levene's heterozygosity (0.6612 -/+ 0.15), expected Nei's heterozygosity (0.6535 -/+ 0.14), and polymorphism information content (0.6120 -/+ 0.03). Low values of Wright's fixation index, F(IS) (0.2433 -/+ 0.05) indicated low levels of inbreeding. This basic study indicated the existence of substantial genetic diversity in the Marwari horse population. No significant genotypic linkage disequilibrium was detected across the population, suggesting no evidence of linkage between loci. A normal 'L' shaped distribution of mode-shift test, non-significant heterozygote excess on the basis of different models, as revealed from Sign, Standardized differences and Wilcoxon sign rank tests as well as non-significant M ratio value suggested that there was no recent bottleneck in the existing Marwari breed population, which is important information for equine breeders. This study also revealed that the Marwari breed can be differentiated from some other exotic breeds of horses on the basis of three microsatellite primers.
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Publication Date: 2005-12-31 PubMed ID: 16385161DOI: 10.1007/BF02715799Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on assessing genetic diversity within the Marwari horse breed using genetic markers, noting high levels of variation and no significant genetic bottlenecking. It also demonstrates that the Marwari breed can be genetically distinguished from other horse breeds.

Objective and Methodology

  • The primary aim of this study was to evaluate the genetic diversity within the Marwari horse breed.
  • The researchers used 26 different microsatellite pairs to analyze the DNA samples from 48 unrelated horses.
  • They also examined the potential issue of genetic bottlenecks in this breed.

Key Findings

  • The researchers discovered a high level of genetic variability in the Marwari horse breed.
  • They observed a total of 133 different alleles with an estimated mean allelic diversity of 5.9.
  • The high values of various parameters such as effective number of alleles, observed heterozygosity, expected Levene’s heterozygosity, expected Nei’s heterozygosity, and polymorphism information content all pointed to this high variability.
  • Due to low values of Wright’s fixation index, F(IS), low levels of inbreeding were indicated in these animals.

Implications

  • The results indicated that there is substantial genetic diversity in the Marwari horse population, suggesting a healthy breeding population with no significant genetic bottlenecks to potentially hinder future genetic diversity.
  • No major genotypic linkage disequilibrium was detected, proving that there is no significant correlation/association between different genetic loci.
  • Three microsatellite primers would be enough to distinguish the Marwari breed from other horse breeds, which might be valuable for breeding and conservation efforts.

Cite This Article

APA
Gupta AK, Chauhan M, Tandon SN. (2005). Genetic diversity and bottleneck studies in the Marwari horse breed. J Genet, 84(3), 295-301. https://doi.org/10.1007/BF02715799

Publication

Journal of genetics
ISSN: 0022-1333
NlmUniqueID: 2985113R
Country: India
Language: English
Volume: 84
Issue: 3
Pages: 295-301

Researcher Affiliations

Gupta, A K
  • National Research Centre on Equines, Sirsa Road, Hisar 125 001, India. akguptanrce@hotmail.com
Chauhan, M
    Tandon, S N

      MeSH Terms

      • Alleles
      • Animals
      • Breeding
      • Genetic Markers
      • Genetic Variation
      • Genetics, Population
      • Horses / blood
      • Horses / genetics
      • Microsatellite Repeats / genetics
      • Models, Theoretical
      • Polymerase Chain Reaction / methods

      References

      This article includes 29 references
      1. Anim Genet. 1997 Dec;28(6):453
        pubmed: 9589589
      2. Anim Genet. 1999 Feb;30(1):71-2
        pubmed: 10050297
      3. Genetics. 1978 Jul;89(3):583-90
        pubmed: 17248844
      4. Anim Genet. 1998 Apr;29(2):153-6
        pubmed: 9699285
      5. Anim Genet. 2000 Feb;31(1):39-48
        pubmed: 10690360
      6. Anim Genet. 2002 Jun;33(3):169-77
        pubmed: 12030919
      7. Mol Ecol. 1998 Aug;7(8):963-74
        pubmed: 9711862
      8. Mol Ecol. 2001 Feb;10(2):305-18
        pubmed: 11298947
      9. Genetics. 1996 Sep;144(1):389-99
        pubmed: 8878702
      10. Anim Genet. 1994 Jun;25(3):207
        pubmed: 7943974
      11. Small Rumin Res. 2001 Jan;39(1):3-10
        pubmed: 11163709
      12. Anim Genet. 2000 Jun;31(3):237-8
        pubmed: 10895325
      13. Proc Natl Acad Sci U S A. 1996 May 14;93(10):5131-5
        pubmed: 8643540
      14. Trends Ecol Evol. 1995 Jul;10(7):267-70
        pubmed: 21237034
      15. Anim Genet. 1997 Feb;28(1):69-70
        pubmed: 9124721
      16. Anim Genet. 1997 Oct;28(5):370-1
        pubmed: 9363603
      17. J Genet. 2004 Dec;83(3):291-5
        pubmed: 15689632
      18. Anim Genet. 1999 Feb;30(1):68-9
        pubmed: 10050293
      19. J Hered. 1998 May-Jun;89(3):238-47
        pubmed: 9656466
      20. Anim Genet. 2002 Dec;33(6):422-7
        pubmed: 12464016
      21. Am J Hum Genet. 1980 May;32(3):314-31
        pubmed: 6247908
      22. Anim Genet. 1996 Apr;27(2):129
        pubmed: 8856915
      23. Anim Genet. 1999 Feb;30(1):70-1
        pubmed: 10050295
      24. Anal Biochem. 1991 Jul;196(1):80-3
        pubmed: 1716076
      25. Genetics. 1996 Dec;144(4):2001-14
        pubmed: 8978083
      26. J Hered. 2000 Nov-Dec;91(6):446-57
        pubmed: 11218082
      27. Anim Genet. 2001 Apr;32(2):59-65
        pubmed: 11421939
      28. Anim Genet. 1992;23(2):133-42
        pubmed: 1443772
      29. Biometrics. 1992 Jun;48(2):361-72
        pubmed: 1637966

      Citations

      This article has been cited 7 times.
      1. Gupta AK, Chauhan M, Bhardwaj A, Vijh RK. Assessment of demographic bottleneck in Indian horse and endangered pony breeds. J Genet 2015 Nov 6;94(4):e56-62.
        doi: 10.1007/s12041-015-0570-5pubmed: 26708456google scholar: lookup
      2. Jun J, Cho YS, Hu H, Kim HM, Jho S, Gadhvi P, Park KM, Lim J, Paek WK, Han K, Manica A, Edwards JS, Bhak J. Whole genome sequence and analysis of the Marwari horse breed and its genetic origin. BMC Genomics 2014;15 Suppl 9(Suppl 9):S4.
        doi: 10.1186/1471-2164-15-S9-S4pubmed: 25521865google scholar: lookup
      3. Gupta AK, Chauhan M, Bhardwaj A. Genetic diversity and bottleneck studies in endangered Bhutia and Manipuri pony breeds. Mol Biol Rep 2013 Dec;40(12):6935-43.
        doi: 10.1007/s11033-013-2813-ypubmed: 24150726google scholar: lookup
      4. Ranjithkumar M, Malik TA, Saxena A, Dan A, Sakthivel PC, Dey S. Hyperlipidaemia in trypanosomiasis of naturally infected horses: possible cachexia-anorexia syndrome?. Trop Anim Health Prod 2013 Feb;45(2):417-21.
        doi: 10.1007/s11250-012-0232-zpubmed: 22836485google scholar: lookup
      5. Bömcke E, Gengler N, Cothran EG. Genetic variability in the Skyros pony and its relationship with other Greek and foreign horse breeds. Genet Mol Biol 2011 Jan;34(1):68-76.
        doi: 10.1590/S1415-47572010005000113pubmed: 21637546google scholar: lookup
      6. Chauhan M, Gupta AK, Dhillon S. Genetic diversity and population structure of three Indian horse breeds. Mol Biol Rep 2011 Jun;38(5):3505-11.
        doi: 10.1007/s11033-010-0461-zpubmed: 21104137google scholar: lookup
      7. Behl R, Behl J, Gupta N, Gupta SC, Ahlawat SP, Ragnekar M, Ahmed Z. Genetic characterization of Zanskari breed of horse. J Genet 2006 Dec;85(3):199-203.
        doi: 10.1007/BF02935331pubmed: 17406094google scholar: lookup
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