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Home / Equine Research Bank / Anim Biosci / Genetic diversity and population structure of Mongolian regi...
Animal bioscience2022; 35(8); 1121-1128; doi: 10.5713/ab.21.0497

Genetic diversity and population structure of Mongolian regional horses with 14 microsatellite markers.

Abstract: This study aimed to identify the genetic diversity and population structure of Mongolian horse populations according to the province of residence (Khentii, KTP; Uvs, USP; Omnogovi and Dundgovi, GOP; Khovsgol, KGP) using 14 microsatellite (MS) markers. Methods: A total of 269 whole blood samples were obtained from the four populations (KTP, USP, GOP, KGP) geographically distinct provinces. Multiplex polymerase chain reaction (PCR) was conducted using 14 MS markers (AHT4, ASB2, ASB17, ASB23, CA425, HMS1, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, and VHL20), as recommended by the International Society for Animal Genetics. Capillary electrophoresis was conducted using the amplified PCR products, alleles were determined. Alleles were used for statistical analysis of genetic variability, Nei's DA genetic distance, principal coordinate analysis (PCoA), factorial corresponding analysis (FCA), and population structure. Results: On average, the number of alleles, expected heterozygosity (HExp), observed heterozygosity (HObs), and polymorphic information content among all populations were 11.43, 0.772, 0.757, and 0.737, respectively. In the PCoA and FCA, GOP, and KGP were genetically distinct from other populations, and the KTP and USP showed a close relationship. The two clusters identified using Nei's DA genetic distance analysis and population structure highlighted the presence of structurally clear genetic separation. Conclusions: Overall, the results of this study suggest that genetic diversity between KTP and USP was low, and that between GOP and KGP was high. It is thought that these results will help in the effective preservation and improvement of Mongolian horses through genetic diversity analysis and phylogenetic relationships.
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Publication Date: 2022-03-02 PubMed ID: 35240022PubMed Central: PMC9262727DOI: 10.5713/ab.21.0497Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the genetic diversity and structure of Mongolian horse populations in different provinces using 14 microsatellite markers in an effort to better understand these populations for more effective preservation and improvement strategies.

Research Methodology

  • The researchers collected a total of 269 blood samples from horses in four distinct Mongolian provinces: Khentii (KTP), Uvs (USP), Omnogovi and Dundgovi (GOP), and Khovsgol (KGP).
  • With these samples, they carried out a multiplex polymerase chain reaction (PCR) using 14 microsatellite markers as recommended by the International Society for Animal Genetics.
  • They then conducted capillary electrophoresis with the amplified PCR products, determining different alleles (variations of a gene) from each sample.
  • These alleles were then used to conduct statistical analysis of genetic variability, genetic distance, principal coordinate analysis, factorial corresponding analysis, and population structure.

Research Findings

  • The analysis showcased that all populations have an average of 11.43 alleles, an expected heterozygosity (a measure of genetic diversity within a population) of 0.772, observed heterozygosity of 0.757, and polymorphic information content (a measure of the discriminative power) of 0.737.
  • The principal coordinate analysis and factorial corresponding analysis showed that the GOP and KGP horse populations are genetically distinct from other populations, while those in KTP and USP are closely related.
  • The clusters identified using genetic distance analysis and population structure showed a clear structural genetic separation.

Conclusions

  • The results of the study reveal a low genetic diversity between the KTP and USP horse populations but a high diversity between the GOP and KGP populations.
  • According to the authors, these findings can help in the effective preservation and improvement of Mongolian horses by providing insight into the genetic diversity and phylogenetic relationship of different regional horse populations in Mongolia.

Cite This Article

APA
Yun J, Oyungerel B, Kong HS. (2022). Genetic diversity and population structure of Mongolian regional horses with 14 microsatellite markers. Anim Biosci, 35(8), 1121-1128. https://doi.org/10.5713/ab.21.0497

Publication

Animal bioscience
ISSN: 2765-0189
NlmUniqueID: 101774366
Country: Korea (South)
Language: English
Volume: 35
Issue: 8
Pages: 1121-1128

Researcher Affiliations

Yun, Jihye
  • Department of Applied Biotechnology, The Graduate School of Hankyong National University, Anseong 17579, Korea.
Oyungerel, Baatartsogt
  • School of Animal Science and Biotechnology, Mongolian University of Life Sciences, Zaisan 17024, Ulaanbaatar, Mongolia.
Kong, Hong Sik
  • Department of Applied Biotechnology, The Graduate School of Hankyong National University, Anseong 17579, Korea.
  • Genomic Informatics Center, Hankyong National University, Anseong 17579, Korea.
  • Gyeonggi Regional Research Center, Hankyong National University, Anseong 17579, Korea.

Conflict of Interest Statement

CONFLICT OF INTEREST. We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

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