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Home / Equine Research Bank / J Equine Vet Sci / Genetic Manipulation of the Equine Oocyte and Embryo.
Journal of equine veterinary science2021; 99; 103394; doi: 10.1016/j.jevs.2021.103394

Genetic Manipulation of the Equine Oocyte and Embryo.

Abstract: As standard in vitro fertilization is not a viable technique in horses yet, many different techniques have been used to create equine embryos for research purposes. One such method is parthenogenesis in which an oocyte is induced to mature into an embryo-like state without the introduction of a spermatozoon, and thus they are not considered true embryos. Another method is somatic cell nuclear transfer (SCNT), in which a somatic cell nucleus from an extant horse is inserted into an enucleated oocyte, creating a genetic clone of the donor horse. Due to limited availability of equine oocytes in the United States, researchers have investigated the potential for combining equine somatic cell nuclei with oocytes from other species to make embryos for research purposes, which has not been successful to date. There has also been a rising interest in producing transgenic animals using sperm exposed to exogenous DNA. The successful creation of transgenic equine blastocysts shows the promise of sperm mediated gene transfer (SMGT), but this method is not ideal for other applications, like gene therapy, because it cannot be used to induce targeted mutations. That is why technologies like CRISPR/Cas9 are vital. In this review, we argue that parthenogenesis, SCNT, and interspecies SCNT can be considered genetic manipulation strategies as they create embryos that are genetically identical to their parent cell. Here, we describe how these methods are performed and their applications and we also describe the few methods that have been used to directly modify equine embryos: SMGT and CRISPR/Cas9.
Copyright © 2021 Elsevier Inc. All rights reserved.
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Publication Date: 2021-02-03 PubMed ID: 33781418PubMed Central: PMC8605602DOI: 10.1016/j.jevs.2021.103394Google Scholar: Lookup
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  • Non-U.S. Gov't
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article provides an overview of various techniques used to genetically manipulate horse oocytes and embryos for research purposes as standard in vitro fertilization isn’t an option for this species. It explores different approaches including parthenogenesis, somatic cell nuclear transfer (SCNT), interspecies SCNT, and the creation of transgenic animals through sperm mediated gene transfer (SMGT) and CRISPR/Cas9 technologies.

Parthenogenesis, SCNT, and interspecies SCNT

  • Parthenogenesis is a process used to induce an oocyte, or an immature egg cell, to mature into an embryo-like state without the involvement of a sperm cell. Such entities aren’t true embryos, though, because they lack genetic material from a father.
  • Somatic cell nuclear transfer (SCNT) is another method described in the paper. It involves transferring the nucleus from a somatic cell of a horse into an oocyte that has had its nucleus removed. This process produces a genetic clone of the horse from which the somatic cell was taken.
  • Due to the limited supply of equine oocytes in the U.S, researchers have investigated the viability of interspecies SCNT – combining horse somatic cell nuclei with oocytes from different species to produce embryos for research. To date, these attempts haven’t been fruitful.

Transgenic animals, SMGT, and CRISPR/Cas9

  • Researchers have increasingly shown interest in producing transgenic animals, i.e., animals carrying a gene that has been artificially inserted into their genome. This process is achieved through sperm mediated gene transfer (SMGT), where sperm cells are exposed to external DNA.
  • The successful creation of transgenic equine blastocysts has proven the potential of SMGT. However, it has its limitations; it can’t be used for specific applications like gene therapy because it doesn’t support the induction of targeted mutations.
  • The authors mention CRISPR/Cas9 technology as a vital tool for this purpose. CRISPR/Cas9 enables targeted modifications to the genome, making it a useful mechanism for various applications in genetic research, including gene therapy.

In their conclusion, the authors argue that parthenogenesis, SCNT, and interspecies SCNT are all forms of genetic manipulation, as they generate embryos that are genetically identical to their parent cell. They also illustrate the use of SMGT and CRISPR/Cas9 for direct modification of equine embryos.

Cite This Article

APA
Hisey EA, Ross PJ, Meyers S. (2021). Genetic Manipulation of the Equine Oocyte and Embryo. J Equine Vet Sci, 99, 103394. https://doi.org/10.1016/j.jevs.2021.103394

Publication

Journal of equine veterinary science
ISSN: 0737-0806
NlmUniqueID: 8216840
Country: United States
Language: English
Volume: 99
Pages: 103394

Researcher Affiliations

Hisey, Erin A
  • Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA.
Ross, Pablo J
  • Department of Animal Science, University of California, Davis, CA.
Meyers, Stuart
  • Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA. Electronic address: smeyers@ucdavis.edu.

MeSH Terms

  • Animals
  • Blastocyst
  • Embryo, Mammalian
  • Horses
  • Male
  • Nuclear Transfer Techniques / veterinary
  • Oocytes
  • Parthenogenesis

Grant Funding

  • T32 GM136559 / NIGMS NIH HHS
  • T35 OD010956 / NIH HHS

Conflict of Interest Statement

Conflicts of Interest: None.

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