Genetic polymorphism of Hucul horse population based on 17 microsatellite loci.
Abstract: Short tandem repeat (STR) loci, i.e. microsatellites are a class of genetic markers commonly used for population studies and parentage control. This study determined the usefulness of microsatellite markers recommended by International Society for Animal Genetics (ISAG) for identification and pedigree analysis in horses based on the example of Polish Hucul horse population (Equus caballus). The set of seventeen microsatellites loci was tested (AHT4, AHT5, ASB2, HMS2, HMS3, HMS6, HMS7, HTG10, HTG4, HTG6, HTG7, VHL20, ASB17, ASB23, CA425, HMS1, LEX3) for 216 individuals. All samples were genotyped and mean number of alleles per locus was estimated (7.00). Means of observed (Ho) and expected (He) heterozygosity were calculated 0.7288 and 0.7027, respectively. The observed heterozygosity was similar to the results of research on Hucul horse population in another area of Carpathians Mountains. The average polymorphism information content (PIC) for analyses of seventeen microsatellite markers indicates the usefulness of this set of markers for Hucul horse parentage testing.
Publication Date: 2014-01-17 PubMed ID: 24432328
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- Journal Article
Summary
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This research investigates the genetics of the Hucul horse population, particularly in Poland, using microsatellite markers. The study emphasizes the effectiveness of these genetic markers for detecting horse identity and mapping their pedigree.
Objective and Methodology
- The central aim of the research was to evaluate the appropriateness of microsatellite markers, recommended by the International Society for Animal Genetics (ISAG), for identification and pedigree analysis of horses. The population of Polish Hucul horses (Equus caballus) was chosen as a representative sample for this study.
- Microsatellites, or short tandem repeat (STR) loci, are a class of genetic markers that are frequently employed for population studies and parentage control.
- The study tested a set of seventeen microsatellites loci in 216 individual horses. These loci included AHT4, AHT5, ASB2, HMS2, HMS3, HMS6, HMS7, HTG10, HTG4, HTG6, HTG7, VHL20, ASB17, ASB23, CA425, HMS1, and LEX3.
Results and Interpretation
- All the sampled horses were genotyped and the mean number of alleles per locus was estimated which turned out to be 7.00.
- The research team calculated the mean of observed heterozygosity (Ho) and expected heterozygosity (He), two key measures in population genetics. These data hint at the genetic diversity within the population. Ho and He were found to be 0.7288 and 0.7027, respectively.
- The observed heterozygosity was found to be similar to what researchers obtained in previous studies conducted on Hucul horse populations in another area of the Carpathian Mountains. This indicates a level of consistency in the genetic makeup across different regions.
- The average Polymorphism Information Content (PIC), a measure often used to gauge the utility of a genetic marker, was calculated for the analyses of seventeen microsatellite markers. Based on these values, the study suggests that this set of markers can be efficiently used for parentage testing of Hucul horses.
Cite This Article
APA
Fornal A, Radko A, Piestrzyńska-Kajtoch A.
(2014).
Genetic polymorphism of Hucul horse population based on 17 microsatellite loci.
Acta Biochim Pol, 60(4), 761-765.
Publication
Researcher Affiliations
- National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland.
- National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland.
- National Research Institute of Animal Production, Department of Cytogenetics and Molecular Genetics of Animals, Balice n. Kraków, Poland.
MeSH Terms
- Alleles
- Animals
- Genetic Markers
- Genotype
- Heterozygote
- Horses / genetics
- Microsatellite Repeats / genetics
- Pedigree
- Polymorphism, Genetic
Citations
This article has been cited 3 times.- Topczewska J, Bartman J, Kwater T. Assessing the utility value of Hucul horses using classification models, based on artificial neural networks.. PLoS One 2022;17(7):e0271340.
- Bażanów B, Pawęska JT, Pogorzelska A, Florek M, Frącka A, Gębarowski T, Chwirot W, Stygar D. Serological Evidence of Common Equine Viral Infections in a Semi-Isolated, Unvaccinated Population of Hucul Horses.. Animals (Basel) 2021 Jul 30;11(8).
- Bażanów BA, Chełmecka E, Romuk E, Stygar DM. Basic Studies on the Oxidative Stress Markers in Two Types of Horse Breed: Semi-isolated Population of Huculs Is Different from Commercially Used Arabian Horses.. Biomed Res Int 2020;2020:7542384.
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