Genetic relationship between Miyako and Yonaguni horses native to Okinawa based on polymorphisms of microsatellites.
Abstract: The Miyako and Yonaguni horses are native horses in Okinawa. Here, we evaluated their genetic relationship using microsatellite data and Kiso horses, which have four subpopulations, as a reference population for evaluating this relationship. Microsatellite data from 35 Miyako, 78 Yonaguni, and 172 Kiso horses were evaluated using the STRUCTURE software for analyzing multilocus genotype data to investigate the population structures and their underlying relationship. The results of the STRUCTURE analysis were stable when ΔK was 2, suggesting that the Okinawan horses are different from the Kiso horses. Moreover, the results were also stable when ΔK was 6; the sample was then divided into four subpopulations of the Kiso horses and two Okinawan horse breeds. However, the diagrams from the STRUCTURE analysis were unstable when ΔK was 3. These results suggest that the genetic relationship of the Okinawan horse breeds may be close, similar to that among the subpopulations of the Kiso horses.
Publication Date: 2018-12-18 PubMed ID: 30607131PubMed Central: PMC6306297DOI: 10.1294/jes.29.87Google Scholar: Lookup
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Summary
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The research article explores the genetic relationship between the Miyako, Yonaguni, and Kiso horses, which are native to Okinawa, using microsatellite data.
Study Purpose and Methodology
- This study aimed to ascertain the genetic relationship between the Miyako and Yonaguni horses, native to Okinawa, by comparing their genomes using microsatellite data. The Kiso horses, which comprise four subpopulations, served as the reference population to evaluate this relationship.
- Microsatellite data from 35 Miyako, 78 Yonaguni, and 172 Kiso horses were analyzed by applying the STRUCTURE software—a specific software for analyzing multilocus genotype data. This was done to investigate the population structures and understand the underlying relationship between these breeds.
Key Findings
- The results of the STRUCTURE analysis consistently suggested when ΔK was 2, indicating that the Okinawan horses (Miyako and Yonaguni) have distinct genetic structures from the Kiso horses.
- The results were also stable when ΔK was 6, implying that the sample could break down into four subpopulations of the Kiso horses and two Okinawan horse breeds. This pattern validates the genetic distinction and diversity among the studied breeds.
- On the other hand, when ΔK was 3, the results from the STRUCTURE analysis were unstable, indicating inconsistency in the classification of these breeds when using this value.
Conclusion
- The study concludes that there’s a close genetic relationship between the Miyako and Yonaguni breeds, similar to that between the subpopulations of the Kiso horses. The analysis, therefore, suggests that despite the geographical difference, the Okinawan horse breeds share significant genetic similarity.
- These findings offer valuable insights into the genetic relationship and lineage of these breeds, thus significantly contributing to biodiversity and conservation efforts associated with these native Okinawan horses.
Cite This Article
APA
Senju N, Tozaki T, Kakoi H, Matsuyama R, Nakamura K, Takasu M.
(2018).
Genetic relationship between Miyako and Yonaguni horses native to Okinawa based on polymorphisms of microsatellites.
J Equine Sci, 29(4), 87-90.
https://doi.org/10.1294/jes.29.87 Publication
Researcher Affiliations
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
- Nagoya Higashiyama Zoo and Botanical Gardens, Aichi 464-0804, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
- Education and Research Center for Food Animal Health (GeFAH), Gifu University, Gifu 501-1193, Japan.
References
This article includes 15 references
- Earl DA, VonHoldt BM. Structure Harvester: a website and program for visualizing STRUCTURE output and implementing the Evanno method.. Cons. Genet. Res. 2012;4:359–361.
- Frankham R, Ballou JD, Briscoe DA. Genetic diversity. 2009;pp. 41–65. In: Introduction to Conservation Genetics, 2nd ed. (Frankham, R., Ballou, J.D., and Brisocoe, D.A. eds.), Cambridge University Press, Cambridge.
- Hashiguchi T. Tokara horse. 1984;pp. 115–130. In: Japanese Native Horses—Its Conservation and Usage, Japan Equine Association, Tokyo (in Japanese).
- Kakoi H, Nagata S, Kurosawa M. DNA typing with 17 microsatellites for parentage verification of racehorses in Japan. Anim. Sci. J. 2001;72:453–460.
- Kakoi H, Tozaki T, Gawahara H. Molecular analysis using mitochondrial DNA and microsatellites to infer the formation process of Japanese native horse populations.. Biochem Genet 2007 Apr;45(3-4):375-95.
- Pritchard JK, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data.. Genetics 2000 Jun;155(2):945-59.
- Scherf BD. Farm animal genetic resources. 2000;pp. 37–646. In: World Watch List of Domestic Animal Diversity, 3rd ed., Food and Agriculture Organization of the United Nations, Rome.
- Senju N, Tozaki T, Kakoi H, Almunia J, Maeda M, Matsuyama R, Takasu M. Genetic characterization of the Miyako horse based on polymorphisms of microsatellites and mitochondrial DNA.. J Vet Med Sci 2017 Jan 24;79(1):218-223.
- Senju N, Tozaki T, Kakoi H, Shinjo A, Matsuyama R, Almunia J, Takasu M. Genetic diversity of the Yonaguni horse based on polymorphisms in microsatellites and mitochondrial DNA.. J Vet Med Sci 2017 Feb 28;79(2):425-431.
- Shinjo A. Miyako horse. 1984;pp. 131–148. In: Japanese Native Horses—Its Conservation and Usage, Japan Equine Association, Tokyo (in Japanese).
- Shinjo A. Yonaguni horse. 1984;pp. 149–154. In: Japanese Native Horses—Its Conservation and Usage, Japan Equine Association, Tokyo (in Japanese).
- Shinjo A. Domestic Animals Native to Okinawa: Their Introduction and Life History. 2010;Border Inc., Naha (in Japanese).
- Takasu M, Hiramatsu N, Tozaki T, Kakoi H, Nakagawa T, Hasegawa T, Huricha, Maeda M, Murase T, Mukoyama H. Genetic characterization of the endangered Kiso horse using 31 microsatellite DNAs.. J Vet Med Sci 2012 Feb;74(2):161-6.
- Tozaki T, Kakoi H, Mashima S, Hirota K, Hasegawa T, Ishida N, Miura N, Choi-Miura NH, Tomita M. Population study and validation of paternity testing for Thoroughbred horses by 15 microsatellite loci.. J Vet Med Sci 2001 Nov;63(11):1191-7.
- Tozaki T, Takezaki N, Hasegawa T, Ishida N, Kurosawa M, Tomita M, Saitou N, Mukoyama H. Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup.. J Hered 2003 Sep-Oct;94(5):374-80.
Citations
This article has been cited 2 times.- Masuda M, Nikadori A, Nikadori E, Uchida N, Takizawa Y, Ishimaru M, Yamamoto T, Yuasa R, Kugai H, Nagahama Y, Takasu M, Tozaki T. Evaluation of genetic diversity using 31 microsatellites in Miyako horses.. J Equine Sci 2023 Mar;34(1):1-6.
- May-Davis S, Minowa F, Brown WY. An in situ and ultrasound study of Yonaguni ponies revealed the rare finding of the nuchal ligament lamellae from C2-C7.. J Equine Sci 2020;31(4):93-94.
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