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Veterinary microbiology1992; 32(2); 101-115; doi: 10.1016/0378-1135(92)90099-f

Genomic variability among globally distributed isolates of equine arteritis virus.

Abstract: Equine arteritis virus (EAV), a non-arthropod borne togavirus, has been shown to have a global distribution. To date, no major antigenic variation has been demonstrated between EAV isolates from different geographic origins. In this study, the genomic RNA of EAV isolates obtained from horses of different breeds in various countries around the world was oligonucleotide fingerprinted. Comparisons of these fingerprints were used to determine the extent of genomic variation among such isolates. Comparisons among isolates from North American horses revealed, for the most part, oligonucleotide homologies of less than 60%. Only 29 of the 98 comparisons revealed greater than 60% oligonucleotide homology. Nonetheless, several comparisons indicated a close epidemiologic relationship between isolates from horses of different breeds located in different states. Though all European isolates were of Standardbred origin and were from horses located in northern European countries, the majority had oligonucleotide homologies of less than 60%. Where oligonucleotide homology was apparent, it was, with one exception, greater than 70%. The two isolates from New Zealand had 93.2% oligonucleotide homology. This is indicative of an extremely close epidemiologic relationship. Comparisons between EAV isolates from around the world revealed oligonucleotide homologies between viruses from North America, Europe and New Zealand. In several instances, this homology was greater than 70% and in one case greater than 80%. No oligonucleotide homology was evident in comparisons involving the virus from South Africa. The high level of genomic conservation between certain EAV isolates of disparate geographic origins may reflect dissemination of the virus associated with the international movement of horses. The extent of genomic variation demonstrated between most of the EAV isolates used in this study confirms the need for further investigation of genomic heterogeneity among strains of this virus before techniques that rely upon nucleic acid hybridization can be effectively applied as diagnostic procedures.
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Publication Date: 1992-09-01 PubMed ID: 1332249DOI: 10.1016/0378-1135(92)90099-fGoogle Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on determining the genomic variability among different global isolates of equine arteritis virus (EAV), a virus observed worldwide among the horse community. Through the use of oligonucleotide fingerprinting, variations between isolates from various locations, breeds, and origins were analyzed.

Methodology

  • The study utilized genomic RNA from EAV isolates, gathered from horses of varying breeds in numerous countries.
  • Oligonucleotide fingerprinting was used to compare these samples. This is a method used to determine viral genetic sequences, and thus identify variations.

Results

  • The research found a majority of isolates from North American horses showed less than 60% oligonucleotide homologies, indicating significant genomic variations.
  • Even though there were variations, several comparisons indicated a close epidemiologic relationship between isolates from horses of different breeds located in different states.
  • The European isolates, although all from Standardbred horses in northern Europe, also predominantly exhibited less than 60% homology.
  • The two isolates from New Zealand displayed 93.2% oligonucleotide homology, suggesting a very close epidemiologic relationship.

Global Comparison

  • When comparing EAV isolates worldwide, homologies between viruses from North America, Europe, and New Zealand were noticed, in multiple cases greater than 70% and in one instance over 80%.
  • No homology was found when comparing the virus isolate from South Africa to other isolates.

Implications and Conclusions

  • The high degree of genomic conservation between certain EAV isolates of disparate geographic origins could reflect the virus’s dissemination associated with the global movement of horses.
  • The extensive genomic variation detected between most of the EAV isolates in the study underscores the need for more investigation of genomic heterogeneity among strains of this virus.
  • The researchers emphasize the importance of understanding these genetic variations before techniques that rely upon nucleic acid hybridization, such as some diagnostic procedures, can be effectively applied.

Cite This Article

APA
Murphy TW, McCollum WH, Timoney PJ, Klingeborn BW, Hyllseth B, Golnik W, Erasmus B. (1992). Genomic variability among globally distributed isolates of equine arteritis virus. Vet Microbiol, 32(2), 101-115. https://doi.org/10.1016/0378-1135(92)90099-f

Publication

Veterinary microbiology
ISSN: 0378-1135
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 32
Issue: 2
Pages: 101-115

Researcher Affiliations

Murphy, T W
  • Gluck Equine Research Center, University of Kentucky, Lexington 40546.
McCollum, W H
    Timoney, P J
      Klingeborn, B W
        Hyllseth, B
          Golnik, W
            Erasmus, B

              MeSH Terms

              • Animals
              • Arteritis / epidemiology
              • Arteritis / microbiology
              • Arteritis / veterinary
              • Autoradiography
              • Breeding
              • Electrophoresis, Gel, Two-Dimensional
              • Equartevirus / genetics
              • Europe / epidemiology
              • Genetic Variation
              • Horse Diseases / epidemiology
              • Horse Diseases / microbiology
              • Horses
              • New Zealand / epidemiology
              • North America / epidemiology
              • Oligonucleotides / analysis
              • RNA, Viral / analysis
              • Sequence Homology, Nucleic Acid
              • South Africa / epidemiology
              • Togaviridae Infections / epidemiology
              • Togaviridae Infections / microbiology
              • Togaviridae Infections / veterinary

              Citations

              This article has been cited 14 times.
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