FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Equine Vet J / Genotypic diversity of Salmonella ser. Abortusequi isolates ...
Equine veterinary journal2019; 52(1); 98-103; doi: 10.1111/evj.13123

Genotypic diversity of Salmonella ser. Abortusequi isolates from Argentina.

Abstract: Salmonella enterica subsp. enterica serovar Abortusequi (S. Abortusequi) is a serotype restricted to equines, which produces abortion outbreaks. Nowadays the disease is being reported in different countries including Argentina thus generating an important impact in the equine industry. Molecular characterization of the 95 kb virulence plasmid and the spvC gene of S. Abortusequi demonstrated their importance in the pathogenicity of the serotype. In the last decades, high clonality of S. Abortusequi was identified in Japan, Mongolia and Croatia. Objective: The aim of this work was to characterize S. Abortusequi isolates obtained in Argentina between 2011 and 2016 by virulence-gene profiling and pulsed-field gel electrophoresis. Methods: Case report. Methods: S. Abortusequi isolates were studied by virulence-gene profiling and pulsed-field gel electrophoresis. Results: Four virulence profiles and nine pulsed-field gel electrophoresis pulsotypes were identified among the 27 isolates included in the study. Different strains were found in the same outbreak and/or farm suggesting the presence of different sources of infection or mutation of isolates. Conclusions: The number of related and nonrelated strains. More isolates may be necessary for a more intensive study. Conclusions: Most strains presented the same virulence profile, being positive for all the studied genes except gipA and sopE1, which are involved in intestinal virulence. Only few isolates showed different results in the same outbreak or farm. Unlike other studies, our results demonstrate a considerable diversity of S. Abortusequi pulsed-field gel electrophoresis pulsotypes, which suggests that different sources of infection may be involved within the same outbreak.
© 2019 EVJ Ltd.
Get Full Text
Publication Date: 2019-05-22 PubMed ID: 30991461DOI: 10.1111/evj.13123Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is focused on the study of the genotypic diversity of Salmonella abortusequi, a virus affecting equines, in Argentina. The study examines the different strains of the virus and their virulence profiles.

Objective and Methods of the study

  • The objective of the research is to study and characterize the strains of Salmonella abortusequi spotted in Argentina between 2011 and 2016.
  • Two methods were employed – virulence-gene profiling, a technique used to characterize and categorize the virulence factors, and pulsed-field gel electrophoresis, a method used for the separation and characterization of large DNA molecules.

Results of the study

  • The study analyses 27 strains of Salmonella abortusequi and identifies four distinct virulence profiles and nine separate pulsed-field gel electrophoresis pulsotypes.
  • It was found that different strains were present in the same outbreak or farm, indicating either multiple sources of infection or mutation of the isolates.
  • The results suggest a considerable diversity of S. Abortusequi pulsed-field gel electrophoresis pulsotypes. This unusual diversity suggests that similar outbreaks may have been instigated by different sources of infection.

Conclusions of the study

  • The research conveys that a more intensive study may require additional isolates to fully understand the related and non-related strains.
  • It was noticed that most strains carried the same virulence profile, being positive for all the studied genes except for the gipA and sopE1 genes, which are involved in intestinal virulence.
  • Merely a few isolates presented differing results within the same outbreak or farm.
  • Contrary to other studies, prominent diversity was found amongst S. Abortusequi pulsed-field gel electrophoresis pulsotypes in Argentina.

Cite This Article

APA
(2019). Genotypic diversity of Salmonella ser. Abortusequi isolates from Argentina. Equine Vet J, 52(1), 98-103. https://doi.org/10.1111/evj.13123

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 52
Issue: 1
Pages: 98-103

Researcher Affiliations

MeSH Terms

  • Animals
  • Argentina / epidemiology
  • Genotype
  • Horse Diseases / epidemiology
  • Horse Diseases / microbiology
  • Horses
  • Salmonella Infections, Animal / epidemiology
  • Salmonella Infections, Animal / microbiology
  • Salmonella enterica / classification
  • Salmonella enterica / genetics
  • Salmonella enterica / pathogenicity
  • Transcriptome
  • Virulence

Grant Funding

  • INTA National Animal Health Program Research Project
  • Consejo Nacional de Investigaciones Cientu00edficas y Tu00e9cnicas (CONICET)

References

This article includes 26 references
  1. Monteverde JJ. Abortos microbianos en la producción equina en la Argentina.. Comunicación del Académico de Número. Acad. Nac. de Agron. y Vet. 2, 5-15.
  2. Hernandez JA, Long MJ, Traub-Dargatz JL, Besser TE. Salmonellosis.. Equine Infectious Diseases 2nd edn., Eds: D.C. Sellon and M.C. Long, Saunders Elsevier, Missouri. pp 321-333.
  3. Niwa H, Hobo S, Kinoshita Y, Muranaka M, Ochi A, Ueno T, Oku K, Hariu K, Katayama Y. Aneurysm of the cranial mesenteric artery as a site of carriage of Salmonella enterica subsp. enterica serovar Abortusequi in the horse.. J. Vet. Diagn. Invest. 28, 440-444.
  4. Stritof Z, Habus J, Grizelj J, Koskovic Z, Barbic LJ, Stevanovic V, Horvatek Tomic D, Milas Z, Perharic M, Staresina V, Turk N. Two outbreaks of Salmonella Abortusequi abortion in mares in Croatia.. J. Equine. Vet. Sci. 39, S63.
  5. Madic J, Hajsig D, Sostaric B, Curic S, Seol B, Naglic T. An outbreak of abortion in mares associated with Salmonella abortusequi infection.. Equine Vet. J. 29, 230-233.
  6. Bustos CP, Gallardo J, Retamar G, Lanza N, Falzoni E, Caffer MI, Picos J, Muñoz A, Pérez A, Moras E, Mesplet M, Guida N. Salmonella enterica serovar Abortusequi as an emergent pathogen causing equine abortion in Argentine.. J. Equine. Vet. Sci. 39, S58-S59.
  7. Di Gennaro EE, Guida N, Franco PG, Moras EV, Muñoz AJ. Infectious abortion caused by Salmonella enterica subsp. enterica serovar Abortusequi in Argentina.. J. Equine. Vet. Sci. 32, S74.
  8. Buigues S, Ivanissevich A, Vissani MA, Viglierchio V, Minantel L, Crespo F, Herrera M, Timoney P, Barrandeuy ME. Outbreak of Salmonella Abortus equi abortion in embryo recipient polo mares.. J. Equine. Vet. Sci. 32, S69-S70.
  9. Grandolfo E, Parisi A, Ricci A, Lorusso E, de Siena R, Trotta A, Buonavoglia D, Martella V, Corrente M. High mortality in foals associated with Salmonella enterica subsp. enterica Abortusequi infection in Italy.. J. Vet. Diagn. Invest. 30, 483-485.
  10. De Toro M, Seral C, Rojo-Bezares B, Torres C, Castillo FJ, Sáenz Y. Resistencia a antibióticos y factores de virulencia en aislados clínicos de Salmonella entérica.. Enferm. Infecc. Microbiol. Clin. 32, 4-10.
  11. Soto SM, Rodríguez I, Rodicio MR, Vila J, Mendoza MC. Detection of virulence determinants in clinical strains of Salmonella enterica serovar Enteritidis and mapping on macrorestriction profiles.. J. Med. Microbiol. 55, 365-373.
  12. Huehn S, La Ragione RM, Anjum M, Saunders M, Woodward MJ, Bunge C, Helmuth R, Hauser E, Guerra B, Beutlich J, Brisabois A, Peters T, Svensson L, Madajczak SG, Litrup E, Imre A, Herrera-Leon S, Mevius D, Newell DG, Malorny B. Virulotyping and antimicrobial resistance typing of Salmonella enterica serovars relevant to human health in Europe.. Foodborne Pathog. Dis. 7, 523-535.
  13. Niwa H, Akiba M, Sekizuka T, Kuroda M, Kinoshita Y, Katayama Y. Determination of whole-genome sequence of Salmonella Abortusequi.. J. Equine. Vet. Sci. 39, S59.
  14. Akiba M, Sameshima T, Anzai T, Wada R, Nakazawa M. Salmonella Abortusequi strains of equine origin harbor a 95 kb plasmid responsible for virulence in mice.. Vet. Microbiol. 68, 265-272.
  15. Akiba M, Uchida I, Nishimori K, Tanaka K, Anzai T, Kuwamoto Y, Wada R, Ohya T, Ito H. Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting.. Vet. Microbiol. 92, 379-388.
  16. Malorny B, Hoorfar J, Bunge C, Helmuth R. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an International Standard.. Appl. Environ. Microbiol. 69, 290-296.
  17. Cowan, S.T., Feltham, R.K., Steel, K.J. and Barrow, G.I. (1993). In: Cowan and Steel's Manual for the Identification of Medical Bacteria, 3th edn., Eds: G.I. Barrow and R.K. Feltham, Cambridge University Press, Cambridge.
  18. Grimont PA, Weill FX. Antigenic Formulae of the Salmonella serovars, 9th edn.. WHO Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur, Paris pp 21-111.
  19. . Standard Operating Procedure for PulseNet PFGE of Escherichia coli O157:H7, Escherichia coli non-O157 (STEC), Salmonella serotypes, Shigella sonnei and Shigella flexneri.. Centers for Disease Control and Prevention 2018.
  20. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.. J. Clin. Microbiol. 33, 2233-2239.
  21. Groisman EA, Ochman H. Pathogenicity Islands: bacterial evolution in quantum leaps.. Cell 87, 791-794.
  22. Osman KM, Elhariri M, Amin ZMS, AlAtfeehy N. Consequences to international trade of chicken hatchlings: Salmonella enterica and its public health implications.. Int. J. Adv. Res. 2, 45-63.
  23. Ben Salem R, Abbassi MS, García V, García-Fierro R, Njoud C, Messadi L, Rodicio MR. Detection and molecular characterization of Salmonella enterica serovar Eppendorf circulating in chicken farms in Tunisia.. Zoonoses Public Health 63, 320-327.
  24. Anzai T, Kuwamoto Y, Hobo S, Niwa H, Katayama Y, Ode H, Abe N, Doi A, Akiba M, Sameshima T. The importance of 95-kv virulence plasmid in the pathogenicity of Salmonella Abortusequi in horses.. J. Equine Sci. 16, 111-116.
  25. Astolfi-Ferreira CS, Pequini MRS, Nuñez LFN, Santander Parra SH, Chacon R, de la Torre DID, Pedroso AC, Piantino Ferreira AJ. A comparative survey between non-systemic Salmonella spp. (paratyphoid group) and systemic Salmonella Pullorum and S. Gallinarum with a focus on virulence genes.. Pesqui. Vet. Bras. 37, 1064-1068.
  26. Suez J, Porwollik S, Dagan A, Marzel A, Schorr YI, Desai PT, Agmon V, McClelland M, Rahav G, Gal-Mor O. Virulence gene profiling and pathogenicity characterization of non-typhoidal Salmonella accounted for invasive disease in humans.. PLoS One 8, e58449.

Citations

This article has been cited 2 times.
  1. Bakishev T, Amirgazin A, Kuibagarov M, Shevtsov A, Bakisheva Z, Yessembekova G, Kairzhanova A, Kadyrov A, Guo K, Wang X, Abdrakhmanov S, Borovikov S. Genome-wide characterization and comparative phylogenomics of three Salmonella Abortusequi strains isolated from equine abortions in Kazakhstan. Vet World 2025 Jun;18(6):1571-1580.
    doi: 10.14202/vetworld.2025.1571-1580pubmed: 40689182google scholar: lookup
  2. Manikandan R, Rajagunalan S, Malmarugan S, Gupta C. First report on whole genome sequencing and comparative genomics of Salmonella enterica serovar Abortusequi isolated from Donkey in India. Sci Rep 2024 Oct 8;14(1):23455.
    doi: 10.1038/s41598-024-73904-9pubmed: 39379477google scholar: lookup
Subscribe to our newsletter
Join 99,000 horse owners like you who receive our email updates on horse health and nutrition.