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BMC veterinary research2017; 13(1); 187; doi: 10.1186/s12917-017-1112-6

Geospatial and temporal associations of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015.

Abstract: We studied a recent epizootic of Getah virus infection among pigs in the southern part of Ibaraki Prefecture and the northern part of Chiba Prefecture, Japan, focusing on its possible association with outbreaks in racehorses in 2014 and 2015. The genomic sequence of a Getah virus strain from an infected pig was analyzed to evaluate the degree of identity with the strains from horses. Results: Sera were collected from pigs from September to December 2012 to 2015 in south Ibaraki (380 pigs in 29 batches), and from September to December 2010 to 2015 in north Chiba (538 pigs in 104 batches). They were examined by using a virus-neutralizing test for Getah virus. Seropositivity rates in 2012-2013 in south Ibaraki and 2010-2012 in north Chiba ranged from 0% to 1.6%. In south Ibaraki, seropositivity rates in 2014 (28.8%) and 2015 (65.0%) were significantly higher than those in the previous years (P < 0.01); 4/5 batches had positive sera in 2014 and 7/7 in 2015. In north Chiba, seropositivity rates in 2013 (14.1%), 2014 (17.8%), and 2015 (48.0%) were significantly higher than those in the previous years (P < 0.01); 6/27 batches had positive sera in 2013, 3/9 in 2014, and 5/5 in 2015. Complete genome analysis revealed that the virus isolated from an infected pig had 99.89% to 99.94% nucleotide identity to the strains isolated from horses during the outbreaks in 2014 and 2015. Conclusions: Serological surveillance of Getah virus in pigs revealed that the virus was circulating in south Ibaraki and north Chiba in 2014 and 2015; this was concomitant with the outbreaks in racehorses. The Getah virus strain isolated from a pig was closely related to the ones from horses during the 2014 and 2015 outbreaks. To our knowledge, this is the first convincing case of simultaneous circulation of Getah virus both among pigs and horses in specific areas.
Publication Date: 2017-06-19 PubMed ID: 28629406PubMed Central: PMC5477264DOI: 10.1186/s12917-017-1112-6Google Scholar: Lookup
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  • Journal Article

Summary

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The research paper investigates a recent outbreak of Getah virus among pigs in Japan and its potential connection with outbreaks of the same virus in racehorses during 2014 and 2015. After performing serological surveillance and completing genome analysis, the researchers found simultaneous circulation of the virus in pigs and horses.

Research Study and Methodology

  • The researchers concentrated on an epizootic or animal epidemic of Getah virus in the southern area of Ibaraki Prefecture and northern part of Chiba Prefecture, Japan.
  • The study focuses on how this pig outbreak could have been associated with Getah virus outbreaks in racehorses in 2014 and 2015.
  • The degree of genetic similarity between the Getah virus strain found in an infected pig and strains found in horses was analysed by studying the genomic sequence.
  • Sera were collected from two batches of pigs in south Ibaraki and north Chiba for several years and were tested for the presence of Getah virus.

Results of the Study

  • Seroposivity rates revealed low prevalence of the virus in 2012-2013 in south Ibaraki and 2010-2012 in north Chiba, with rates ranging between 0 – 1.6%.
  • There was a significant increase in seropositivity rates in the later years, indicating a higher prevalence of Getah virus, especially in south Ibaraki in 2014 (28.8%) and 2015 (65.0%) and north Chiba in 2013 (14.1%), 2014 (17.8%), and 2015 (48.0%).
  • Genome analysis showed that the Getah virus strain isolated from an infected pig had a 99.89% to 99.94% genetic match to the strains isolated from racehorses during 2014 and 2015 outbreaks.

Conclusions

  • The research concluded that the Getah virus was circulating among pigs in south Ibaraki and north Chiba during the same time as outbreaks among racehorses were taking place in the years 2014 and 2015.
  • The Getah virus strain isolated from the pig was found to be nearly identical to the one from racehorses, suggesting a strong relation between the two outbreaks.
  • This study is the first to provide compelling evidence for the concurrent circulation of the Getah virus among pig and horse populations in certain areas.

Cite This Article

APA
Bannai H, Nemoto M, Niwa H, Murakami S, Tsujimura K, Yamanaka T, Kondo T. (2017). Geospatial and temporal associations of Getah virus circulation among pigs and horses around the perimeter of outbreaks in Japanese racehorses in 2014 and 2015. BMC Vet Res, 13(1), 187. https://doi.org/10.1186/s12917-017-1112-6

Publication

ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 187
PII: 187

Researcher Affiliations

Bannai, Hiroshi
  • Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan. bannai@equinst.go.jp.
Nemoto, Manabu
  • Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
Niwa, Hidekazu
  • Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
Murakami, Satoshi
  • Thermo Fisher Scientific, Life Technologies Japan Ltd, 4-2-8 Shibaura, Minato-ku, Tokyo, Japan.
Tsujimura, Koji
  • Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
Yamanaka, Takashi
  • Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan.
Kondo, Takashi
  • Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi, 329-0412, Japan.

MeSH Terms

  • Alphavirus / classification
  • Alphavirus / isolation & purification
  • Alphavirus Infections / epidemiology
  • Alphavirus Infections / transmission
  • Alphavirus Infections / veterinary
  • Animals
  • Chlorocebus aethiops
  • DNA, Viral
  • Disease Outbreaks / veterinary
  • Genome, Viral
  • Horse Diseases / epidemiology
  • Horse Diseases / transmission
  • Horse Diseases / virology
  • Horses
  • Japan / epidemiology
  • Phylogeny
  • RNA, Viral / blood
  • Sequence Analysis, DNA
  • Species Specificity
  • Swine
  • Swine Diseases / epidemiology
  • Swine Diseases / transmission
  • Swine Diseases / virology
  • Vero Cells

References

This article includes 16 references
  1. Fukunaga Y, Kumanomido T, Kamada M. Getah virus as an equine pathogen.. Vet Clin North Am Equine Pract 2000 Dec;16(3):605-17.
    doi: 10.1016/S0749-0739(17)30099-8pubmed: 11219353google scholar: lookup
  2. Yago K, Hagiwara S, Kawamura H, Narita M. A fatal case in newborn piglets with Getah virus infection: isolation of the virus.. Nihon Juigaku Zasshi 1987 Dec;49(6):989-94.
    doi: 10.1292/jvms1939.49.989pubmed: 2828741google scholar: lookup
  3. Izumida A, Takuma H, Inagaki S, Kubota M, Hirahara T, Kodama K, Sasaki N. Experimental infection of Getah virus in swine.. Nihon Juigaku Zasshi 1988 Jun;50(3):679-84.
    doi: 10.1292/jvms1939.50.679pubmed: 3210480google scholar: lookup
  4. Kumanomido T, Wada R, Kanemaru T, Kamada M, Hirasawa K, Akiyama Y. Clinical and virological observations on swine experimentally infected with Getah virus.. Vet Microbiol 1988 Mar;16(3):295-301.
    doi: 10.1016/0378-1135(88)90033-8pubmed: 2836997google scholar: lookup
  5. Kamada M, Wada R, Kumanomido T, Imagawa H, Sugiura T, Fukunaga Y. Effect of viral inoculum size on appearance of clinical signs in equine Getah virus infection.. J Vet Med Sci 1991 Oct;53(5):803-6.
    doi: 10.1292/jvms.53.803pubmed: 1661174google scholar: lookup
  6. Sentsui H, Kono Y. An epidemic of Getah virus infection among racehorses: isolation of the virus.. Res Vet Sci 1980 Sep;29(2):157-61.
    pubmed: 6258204
  7. Kumanomido T, Fukunaga Y, Ando Y, Kamada M, Imagawa H, Wada R, Akiyama Y, Tanaka Y, Kobayashi M, Ogura N. Getah virus isolations from mosquitoes in an enzootic area in Japan.. Nihon Juigaku Zasshi 1986 Dec;48(6):1135-40.
    doi: 10.1292/jvms1939.48.1135pubmed: 2881020google scholar: lookup
  8. Nemoto M, Bannai H, Tsujimura K, Kobayashi M, Kikuchi T, Yamanaka T, Kondo T. Getah Virus Infection among Racehorses, Japan, 2014.. Emerg Infect Dis 2015 May;21(5):883-5.
    doi: 10.3201/eid2105.141975pmc: PMC4412242pubmed: 25898181google scholar: lookup
  9. Bannai H, Ochi A, Nemoto M, Tsujimura K, Yamanaka T, Kondo T. A 2015 outbreak of Getah virus infection occurring among Japanese racehorses sequentially to an outbreak in 2014 at the same site.. BMC Vet Res 2016 Jun 10;12:98.
    doi: 10.1186/s12917-016-0741-5pmc: PMC4902926pubmed: 27286658google scholar: lookup
  10. Bannai H, Nemoto M, Ochi A, Kikuchi T, Kobayashi M, Tsujimura K, Yamanaka T, Kondo T. Epizootiological Investigation of Getah Virus Infection among Racehorses in Japan in 2014.. J Clin Microbiol 2015 Jul;53(7):2286-91.
    doi: 10.1128/JCM.00550-15pmc: PMC4473224pubmed: 25972425google scholar: lookup
  11. Wekesa SN, Inoshima Y, Murakami K, Sentsui H. Genomic analysis of some Japanese isolates of Getah virus.. Vet Microbiol 2001 Nov 8;83(2):137-46.
    doi: 10.1016/S0378-1135(01)00417-5pubmed: 11557154google scholar: lookup
  12. Nemoto M, Bannai H, Tsujimura K, Yamanaka T, Kondo T. Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan.. Arch Virol 2016 Jun;161(6):1691-5.
    doi: 10.1007/s00705-016-2840-9pubmed: 27016932google scholar: lookup
  13. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.. Mol Biol Evol 2016 Jul;33(7):1870-4.
    doi: 10.1093/molbev/msw054pmc: PMC8210823pubmed: 27004904google scholar: lookup
  14. Kobayashi D, Isawa H, Ejiri H, Sasaki T, Sunahara T, Futami K, Tsuda Y, Katayama Y, Mizutani T, Minakawa N, Ohta N, Sawabe K. Complete Genome Sequencing and Phylogenetic Analysis of a Getah Virus Strain (Genus Alphavirus, Family Togaviridae) Isolated from Culex tritaeniorhynchus Mosquitoes in Nagasaki, Japan in 2012.. Vector Borne Zoonotic Dis 2016 Dec;16(12):769-776.
    doi: 10.1089/vbz.2016.2017pubmed: 27827562google scholar: lookup
  15. Kumanomido T, Fukunaga Y, Ando Y, Kamada M, Imagawa H, Wada R. Ecological survey on Getah virus among swine in Japan. Bull Equine Res Inst 1982;19:89–92.
  16. Hohdatsu T, Ide S, Yamagishi H, Eiguchi Y, Nagano H, Maehara N, Tanaka Y, Fujisaki Y, Yago K, Taguchi K. Enzyme-linked immunosorbent assay for the serological survey of Getah virus in pigs.. Nihon Juigaku Zasshi 1990 Aug;52(4):835-7.
    doi: 10.1292/jvms1939.52.835pubmed: 2167998google scholar: lookup

Citations

This article has been cited 12 times.
  1. Yuan Y, Hao Y, Peng C, Zhang D, Ma W, Xiao P, Li N. From transmission to adaptive evolution: genomic surveillance of Getah virus. Front Cell Infect Microbiol 2025;15:1513392.
    doi: 10.3389/fcimb.2025.1513392pubmed: 40535537google scholar: lookup
  2. Shen J, Liu S, Liu S, Shen S, Lei M, Xu Q, Li W, He Q, Xu X, Cai X. Genomic surveillance and evolution of Getah virus. Virus Evol 2025;11(1):veaf007.
    doi: 10.1093/ve/veaf007pubmed: 39989716google scholar: lookup
  3. Jian Z, Jiang C, Zhu L, Li F, Deng L, Ai Y, Lai S, Xu Z. Infectivity and pathogenesis characterization of getah virus (GETV) strain via different inoculation routes in mice. Heliyon 2024 Jul 15;10(13):e33432.
    doi: 10.1016/j.heliyon.2024.e33432pubmed: 39040396google scholar: lookup
  4. Azerigyik FA, Faizah AN, Kobayashi D, Amoa-Bosompem M, Matsumura R, Kai I, Sasaki T, Higa Y, Isawa H, Iwanaga S, Ishino T. Evaluating the mosquito host range of Getah virus and the vector competence of selected medically important mosquitoes in Getah virus transmission. Parasit Vectors 2023 Mar 15;16(1):99.
    doi: 10.1186/s13071-023-05713-4pubmed: 36922882google scholar: lookup
  5. Ochi A, Bannai H, Aonuma H, Kanuka H, Uchida-Fujii E, Kinoshita Y, Ohta M, Kambayashi Y, Tsujimura K, Ueno T, Nemoto M. Surveillance of Getah virus in mosquitoes and racehorses from 2016 to 2019 at a training center in Ibaraki Prefecture, Japan, a site of several previous Getah virus outbreaks. Arch Virol 2023 Jan 7;168(2):35.
    doi: 10.1007/s00705-022-05631-3pubmed: 36609628google scholar: lookup
  6. Li B, Wang H, Liang G. Getah Virus (Alphavirus): An Emerging, Spreading Zoonotic Virus. Pathogens 2022 Aug 20;11(8).
    doi: 10.3390/pathogens11080945pubmed: 36015065google scholar: lookup
  7. Shi N, Qiu X, Cao X, Mai Z, Zhu X, Li N, Zhang H, Zhang J, Li Z, Shaya N, Lu H, Jin N. Molecular and serological surveillance of Getah virus in the Xinjiang Uygur Autonomous Region, China, 2017-2020. Virol Sin 2022 Apr;37(2):229-237.
    doi: 10.1016/j.virs.2022.02.004pubmed: 35527224google scholar: lookup
  8. Fang Y, Zhang W, Xue JB, Zhang Y. Monitoring Mosquito-Borne Arbovirus in Various Insect Regions in China in 2018. Front Cell Infect Microbiol 2021;11:640993.
    doi: 10.3389/fcimb.2021.640993pubmed: 33791242google scholar: lookup
  9. Ren T, Mo Q, Wang Y, Wang H, Nong Z, Wang J, Niu C, Liu C, Chen Y, Ouyang K, Huang W, Wei Z. Emergence and Phylogenetic Analysis of a Getah Virus Isolated in Southern China. Front Vet Sci 2020;7:552517.
    doi: 10.3389/fvets.2020.552517pubmed: 33344520google scholar: lookup
  10. Shi N, Li LX, Lu RG, Yan XJ, Liu H. Highly Pathogenic Swine Getah Virus in Blue Foxes, Eastern China, 2017. Emerg Infect Dis 2019 Jun;25(6):1252-1254.
    doi: 10.3201/eid2506.181983pubmed: 31107236google scholar: lookup
  11. Sam SS, Teoh BT, Chee CM, Mohamed-Romai-Noor NA, Abd-Jamil J, Loong SK, Khor CS, Tan KK, AbuBakar S. A quantitative reverse transcription-polymerase chain reaction for detection of Getah virus. Sci Rep 2018 Dec 5;8(1):17632.
    doi: 10.1038/s41598-018-36043-6pubmed: 30518924google scholar: lookup
  12. Nemoto M, Bannai H, Ochi A, Niwa H, Murakami S, Tsujimura K, Yamanaka T, Kokado H, Kondo T. Complete Genome Sequences of Getah Virus Strains Isolated from Horses in 2016 in Japan. Genome Announc 2017 Aug 3;5(31).
    doi: 10.1128/genomeA.00750-17pubmed: 28774985google scholar: lookup