GPX5 orthologs of the mouse epididymis-restricted and sperm-bound selenium-independent glutathione peroxidase are not expressed with the same quantitative and spatial characteristics in large domestic animals.
Abstract: We report here on the cloning of cDNAs coding bovine and equine orthologs of mouse epididymis-restricted and sperm-bound glutathione peroxidase 5 (GPX5), a selenium-independent member of the multigenic GPX family in mammals. The complete sequence of bovine GPX5 as well as a partial sequence of the equine GPX5 were characterized, conceptually translated and aligned with other known mammalian GPX5 proteins. Using Northern blotting assays, we show that the level of expression of GPX5 is high in bovine but low in equine and that in both species the regionalization of GPX5 expression in epididymis is not totally identical to what was reported for rodent mouse GPX5. An antibody was produced against GPX5 and used in Western blot assays as well as in immunohistochemistry assays on bovine epididymis sections. It shows that the protein is essentially present in the cytoplasmic compartment of the caput segment 2 epithelium of the bovine epididymis. Unlike in the mouse model, bovine GPX5 seems to be poorly secreted and does not seem to be present on cauda epididymal spermatozoa.
Publication Date: 2005-03-05 PubMed ID: 16054503DOI: 10.1016/j.theriogenology.2005.01.008Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research article discusses the differences in expression and characteristics of a protein called glutathione peroxidase 5 (GPX5) in large domestic animals, such as cattle and horses, compared to mice. GPX5 is primarily found in the epididymis and sperm of mammals.
Experiment Overview
- The researchers cloned the cDNAs (complementary DNAs, reverse transcribed from messenger RNA) coding for bovine and equine GPX5. The whole sequence for bovine GPX5 and a part of the equine GPX5 were sequenced and compared with known GPX5 sequences in other mammals.
Findings on Expression Levels and Regionalization
- Via Northern blotting assays (a technique to detect specific RNA molecules), it was found that GPX5 expression was high in cattle, but low in horses. Further, the regional localization of GPX5 within the epididymis of these animals differed from what was previously reported for the mouse rodent’s epididymis.
- GPX5 was found to be essentially present in the cytoplasmic compartment of the caput segment 2 epithelium of the bovine epididymis. The caput is one part of the epididymis, a duct behind the testis where sperm mature and are stored.
Findings on Protein Secretion
- When an antibody was produced against GPX5 and used in Western blot assays (a technique to detect specific proteins within a sample) and immunohistochemistry assays (a method used to visualize cellular components), it was found that, unlike in the mouse model, bovine GPX5 appears to be poorly secreted and does not seem to be present on the cauda epididymal spermatozoa. The cauda epididymis is another section of the epididymis, where sperm are stored before ejaculation.
Summary
- In essence, the research found considerable heterogeneity in how GPX5 is expressed and localized across different mammal species. The differences in characteristics include its quantitative expression levels, spatial characteristics within the epididymis, and secretion behavior. Specifically, it seems that GPX5 in large domestic animals like cows and horses does not behave in the same way as mouse GPX5.
Cite This Article
APA
Grignard E, Morin J, Vernet P, Drevet JR.
(2005).
GPX5 orthologs of the mouse epididymis-restricted and sperm-bound selenium-independent glutathione peroxidase are not expressed with the same quantitative and spatial characteristics in large domestic animals.
Theriogenology, 64(4), 1016-1033.
https://doi.org/10.1016/j.theriogenology.2005.01.008 Publication
Researcher Affiliations
- Laboratoire Epididyme & Maturation des Gamètes, Université Blaise Pascal, UMR CNRS 6547-GEEM, 24 avenue des Landais, 63177 Aubière cedex, France.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cattle
- Cloning, Molecular
- DNA, Complementary / genetics
- Epididymis / enzymology
- Gene Expression
- Glutathione Peroxidase / analysis
- Glutathione Peroxidase / genetics
- Horses
- Immunohistochemistry
- Isoenzymes / analysis
- Isoenzymes / genetics
- Male
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sequence Alignment
- Species Specificity
- Spermatozoa / enzymology
- Testicular Hormones / genetics
Citations
This article has been cited 7 times.- Bao YJ, Zhou Q, Yu X, Yu X, Castellino FJ. Analysis and Characterization of Glutathione Peroxidases in an Environmental Microbiome and Isolated Bacterial Microorganisms. J Microbiol Biotechnol 2023 Mar 28;33(3):299-309.
- Dhama K, Latheef SK, Dadar M, Samad HA, Munjal A, Khandia R, Karthik K, Tiwari R, Yatoo MI, Bhatt P, Chakraborty S, Singh KP, Iqbal HMN, Chaicumpa W, Joshi SK. Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values. Front Mol Biosci 2019;6:91.
- Luan Z, Fan X, Song H, Li R, Zhang W, Zhang J. Testosterone promotes GPX5 expression of goat epididymal epithelial cells cultured in vitro. In Vitro Cell Dev Biol Anim 2019 Oct;55(9):677-685.
- Barranco I, Tvarijonaviciute A, Perez-Patiño C, Vicente-Carrillo A, Parrilla I, Ceron JJ, Martinez EA, Rodriguez-Martinez H, Roca J. Glutathione Peroxidase 5 Is Expressed by the Entire Pig Male Genital Tract and Once in the Seminal Plasma Contributes to Sperm Survival and In Vivo Fertility. PLoS One 2016;11(9):e0162958.
- Rananaware P, Singh S, Brahmkhatri VP. Scavenging of reactive oxygen and nitrogen species using nanoparticles and their applications in disease management. RSC Adv 2025 Dec 3;15(56):47955-47980.
- Neuman NM, Gilun P, Koziorowska-Gilun M, Janiszewski P, Dziekońska A. Antioxidant Enzyme Activity and mRNA Expression in the Reproductive Tissues of Male European Red Deer (Cervus elaphus elaphus). Int J Mol Sci 2025 Jul 25;26(15).
- Huang J, Li S, Yang Y, Li C, Zuo Z, Zheng R, Chai J, Jiang S. GPX5-Enriched Exosomes Improve Sperm Quality and Fertilization Ability. Int J Mol Sci 2024 Sep 30;25(19).
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