Haematological, bone marrow and clinical chemical changes in neonatal foals given canine recombinant granulocyte-colony stimulating factor.
Abstract: Five Thoroughbred and Quarter Horse cross foals were given 20 micrograms canine recombinant granulocyte-colony stimulating factor (rcG-CSF) per kg bwt intramuscularly (i.m.) on the day of birth and 10 micrograms rcG-CSF/kg for 13 additional days. During this time and for an additional 21 days haematology, bone marrow and clinical chemical analyses were performed. After one day of rcG-CSF administration leucocyte and neutrophil counts increased from 9.16 x 10(9)/l to 23.44 x 10(9)/l and from 6.45 x 10(9)/l to 19.61 x 10(9)/l, respectively. The counts continued to increase for the next 3-4 days and then there was a slight decrease. A second increase followed and the leucocyte and neutrophil counts increased to 52.84 x 10(9)/l and 45.16 x 10(9)/l on the day after the last rcG-CSF administration (Day 15). The counts decreased rapidly immediately after the administration of rcG-CSF was stopped and then at a slower rate. The cell counts were still higher than in the controls at the end of the study period (Day 35). Bone marrow cellularity increased from 10-25% before rcG-CSF was given to 60-80% after 5 days. The increase in cellularity was due to increased myeloid activity because the myeloid to erythroid ratio increased from 2.7 to 8.8. Serum chemistry changes were minimal although foals given rcG-CSF at various times had lower glucose concentrations and increased alkaline phosphatase and gamma glutamyl transferase activities.
Publication Date: 1994-07-01 PubMed ID: 8575399DOI: 10.1111/j.2042-3306.1994.tb04392.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the impact of administering canine recombinant granulocyte-colony stimulating factor (rcG-CSF) on haematological, bone marrow, and clinical chemical parameters in newborn foals.
Overview of Research
This experimental research was conducted on five Thoroughbred and Quarter Horse cross foals. The neonate foals were administered canine recombinant granulocyte-colony stimulating factor (rcG-CSF) and monitored for changes over a period of 35 days, where:
- 20 micrograms (per kg body weight) rcG-CSF was administered on the day of their birth.
- 10 micrograms (per kg body weight) rcG-CSF was administered for the next 13 days.
Key biological and physiological parameters were monitored and analysed, including haematological, bone marrow, and clinical chemical data.
Haematological Changes
Leukocyte and Neutrophil Counts
- Following a single day of rcG-CSF administration, leukocyte and neutrophil counts increased significantly. The leukocyte levels rose from 9.16 x 10(9)/l to 23.44 x 10(9)/l, while the neutrophil levels rose from 6.45 x 10(9)/l to 19.61 x 10(9)/l.
- These counts continued to increase for the next 3-4 days, followed by a slight decrease.
- A second spike occurred after the last day of rcG-CSF administration (Day 15), with leukocyte and neutrophil counts rising to 52.84 x 10(9)/l and 45.16 x 10(9)/l respectively.
- Upon discontinuing the administration of rcG-CSF, these counts decreased rapidly initially, and then more slowly afterwards. The counts still remained higher than the controls at the end of the study period (Day 35).
Bone Marrow Changes
The cellularity of the bone marrow increased significantly post rcG-CSF administration:
- The cellularity increased from 10-25% to 60-80% after five days of rcG-CSF administration.
- This increased cellularity was attributed to increased myeloid activity, as the myeloid-to-erythroid ratio rose from 2.7 to 8.8.
Clinical Chemical Changes
Certain clinical chemical parameters also changed post rcG-CSF administration:
- The foals exhibited lower glucose concentrations.
- There was an increase in the activities of certain enzymes, specifically alkaline phosphatase and gamma glutamyl transferase.
Cite This Article
APA
Zinkl JG, Madigan JE, Fridmann DM, Kabbur MB, Reynal-O'Connor J, Andresen JW.
(1994).
Haematological, bone marrow and clinical chemical changes in neonatal foals given canine recombinant granulocyte-colony stimulating factor.
Equine Vet J, 26(4), 313-318.
https://doi.org/10.1111/j.2042-3306.1994.tb04392.x Publication
Researcher Affiliations
- Department of Clinical Pathology, School of Veterinary Medicine, University of California, Davis 95691, USA.
MeSH Terms
- Alkaline Phosphatase / analysis
- Alkaline Phosphatase / blood
- Animals
- Animals, Newborn / blood
- Animals, Newborn / metabolism
- Biopsy, Needle
- Blood Cell Count / drug effects
- Blood Cell Count / veterinary
- Blood Chemical Analysis / veterinary
- Blood Glucose / analysis
- Bone Marrow / drug effects
- Bone Marrow / pathology
- Dogs
- Granulocyte Colony-Stimulating Factor / pharmacology
- Horses / blood
- Horses / metabolism
- Leukocyte Count / drug effects
- Leukocyte Count / veterinary
- Neutrophils / cytology
- Neutrophils / drug effects
- Platelet Count / drug effects
- Platelet Count / veterinary
- Recombinant Proteins / pharmacology
- gamma-Glutamyltransferase / analysis
- gamma-Glutamyltransferase / blood
Citations
This article has been cited 2 times.- Dascalu MA, Daraban Bocaneti F, Soreanu O, Tutu P, Cozma A, Morosan S, Tanase O. Filgrastim Efficiency in Cats Naturally Infected with Feline Panleukopenia Virus. Animals (Basel) 2024 Dec 11;14(24).
- Wong DM, Alcott CJ, Clark SK, Jones DE, Fisher PG, Sponseller BA. Alloimmune neonatal neutropenia and neonatal isoerythrolysis in a Thoroughbred colt. J Vet Diagn Invest 2012 Jan;24(1):219-26.
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