Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review.
Abstract: The mammalian ovary is responsible for essential stages of folliculogenesis and hormonal production, regulating the female physiological functions during the menstrual/estrous cycles. The mare has been considered an attractive model for comparative studies due to the striking similarities shared with women regarding in vivo and in vitro folliculogenesis. The ovarian follicular population in horses contains a large number of oocytes enclosed in preantral follicles that are yet to be explored. Therefore, the in vitro manipulation of equine preantral follicles aims to avoid the process of atresia and promote the development of follicles with competent oocytes. In this regard, after ovarian tissue harvesting, the use of appropriate processing techniques, as well as suitable approaches to evaluating equine preantral follicles and ovarian tissue, are necessary. Although high-quality equine ovarian tissue can be obtained from several sources, some critical aspects, such as the age of the animals, ovarian cyclicity, reproductive phase, and the types of ovarian structures, should be considered. Therefore, this review will focus on providing an update on the most current advances concerning the critical factors able to influence equine preantral follicle quality and quantity. Also, the in vivo strategies used to harvest equine ovarian tissue, the approaches to manipulating ovarian tissue post-harvesting, the techniques for processing ovarian tissue, and the classical approaches used to evaluate preantral follicles will be discussed.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-07-02 PubMed ID: 32905900DOI: 10.1016/j.theriogenology.2020.06.044Google Scholar: Lookup
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- Journal Article
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Summary
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The research article provides an overview of the progress in the manipulation of equine preantral follicles in vitro. The study explores factors influencing the quality and quantity of these follicles and assesses the various techniques for harvesting and processing equine ovarian tissue.
In Vitro Manipulation of Equine Preantral Follicles
- The research focuses on in vivo and in vitro study of folliculogenesis in horses. Folliculogenesis is the maturation of the ovarian follicle, a crucial process in female mammals relating to the menstrual cycle.
- The aim of the research is to avoid the process of atresia – the natural degeneration of the ovarian follicle – and encourage the development of competent oocytes capable of fertilization.
- The study finds horses to be an ideal model for comparative studies due to their similarities to women in terms of folliculogenesis.
Harvesting and Processing Techniques
- After the equine ovarian tissue is harvested, it goes through a series of processing techniques to isolate and evaluate preantral follicles.
- The research stresses the importance of obtaining high-quality ovarian tissue and thus the selection of appropriate harvesting and processing techniques is fundamental.
- Different factors, including the animal’s age, ovary cyclicity, reproductive phase, and types of ovarian structures, should be kept in mind as they can critically affect the quality of the harvested ovarian tissue.
Evaluating Preantral Follicles
- Once the ovarian tissue is processed and preantral follicles are isolated, they undergo evaluation for their quality and quantity.
- These evaluations are vital to determine the success of the in vitro manipulation and subsequent potential for competent oocyte development.
- The review discusses the traditional approaches used to evaluate these follicles along with the latest developments in evaluation strategies.
Cite This Article
APA
Gastal EL, Aguiar FLN, Gastal GDA, Alves KA, Alves BG, Figueiredo JR.
(2020).
Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review.
Theriogenology, 156, 283-295.
https://doi.org/10.1016/j.theriogenology.2020.06.044 Publication
Researcher Affiliations
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA. Electronic address: egastal@siu.edu.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil; Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Sousa, Paraíba, Brazil.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; National Institute of Agricultural Research, INIA La Estanzuela, Colonia, Uruguay.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil; Postgraduate Program of Gynecology and Obstetrics, Medical School, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
- Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA; Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil; Animal Bioscience Postgraduate Program, Federal University of Goiás, Jataí, Goiás, Brazil.
- Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Ceará, Brazil.
MeSH Terms
- Animals
- Estrus
- Female
- Horses
- Oocytes
- Ovarian Follicle
- Ovary
- Tissue and Organ Harvesting / veterinary
Citations
This article has been cited 4 times.- Hyde KA, Aguiar FLN, Alvarenga PB, Rezende AL, Alves BG, Alves KA, Gastal GDA, Gastal MO, Gastal EL. Characterization of preantral follicle clustering and neighborhood patterns in the equine ovary. PLoS One 2022;17(10):e0275396.
- Hyde KA, Aguiar FLN, Alves BG, Alves KA, Gastal GDA, Gastal MO, Gastal EL. Preantral follicle population and distribution in the horse ovary. Reprod Fertil 2022 Apr 1;3(2):90-102.
- Souza SS, Aguiar FLN, Alves BG, Alves KA, Brandão FAS, Brito DCC, Raposo RDS, Gastal MO, Rodrigues APR, Figueiredo JR, Teixeira DÍA, Gastal EL. Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF. Reprod Fertil 2021 Dec;2(4):251-266.
- Requena F, Campos MJAPM, Martínez Marín AL, Camacho R, Giráldez-Pérez RM, Agüera EI. Assessment of Age Effects on Ovarian Hemodynamics Using Doppler Ultrasound and Progesterone Concentrations in Cycling Spanish Purebred Mares. Animals (Basel) 2021 Aug 8;11(8).
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