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Home / Equine Research Bank / Int J Mol Sci / Heat Shock Alters the Proteomic Profile of Equine Mesenchyma...
International journal of molecular sciences2022; 23(13); 7233; doi: 10.3390/ijms23137233

Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells.

Abstract: The aim of this research was to determine the impact of heat stress on cell differentiation in an equine mesenchymal stem cell model (EMSC) through the application of heat stress to primary EMSCs as they progressed through the cell specialization process. A proteomic analysis was performed using mass spectrometry to compare relative protein abundances among the proteomes of three cell types: progenitor EMSCs and differentiated osteoblasts and adipocytes, maintained at 37 °C and 42 °C during the process of cell differentiation. A cell-type and temperature-specific response to heat stress was observed, and many of the specific differentially expressed proteins were involved in cell-signaling pathways such as Notch and Wnt signaling, which are known to regulate cellular development. Furthermore, cytoskeletal proteins profilin, DSTN, SPECC1, and DAAM2 showed increased protein levels in osteoblasts differentiated at 42 °C as compared with 37 °C, and these cells, while they appeared to accumulate calcium, did not organize into a whorl agglomerate as is typically seen at physiological temperatures. This altered proteome composition observed suggests that heat stress could have long-term impacts on cellular development. We propose that this in vitro stem cell culture model of cell differentiation is useful for investigating molecular mechanisms that impact cell development in response to stressors.
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Publication Date: 2022-06-29 PubMed ID: 35806237PubMed Central: PMC9267023DOI: 10.3390/ijms23137233Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studies the effect of heat stress on the differentiation of equine mesenchymal stem cells (EMSCs), finding that it alters the proteomic profile of the cells. By comparing protein abundances under heat stress, the study observes a cell-type-specific response with potential long-term impacts on cellular development.

Objective of Research

  • The goal of this study was to understand the effect of heat stress on cell differentiation using equine mesenchymal stem cells (EMSCs).
  • Researchers applied heat stress to the EMSCs during the cell specialization/differentiation process to observe the changes.
  • This research aims to elucidate how environmental stressors, such as heat, can influence cellular development.

Research Methodology

  • The researchers compared the proteomes of three cell types: progenitor EMSCs and differentiated osteoblasts and adipocytes.
  • The cells were maintained at two different temperatures, 37°C (normal body temperature) and 42°C (heat stress condition), throughout the process of cell differentiation.
  • The proteomic analysis was performed using mass spectrometry to compare the relative protein abundances.

Findings of the Research

  • The study found a cell-type and temperature-specific response to heat stress.
  • Many of the differentially expressed proteins were found to be involved in cell-signaling pathways like Notch and Wnt signaling. These pathways are known to regulate cellular development.
  • Heat stress also caused an increase in protein levels in osteoblasts, differentiated at 42 °C, for cytoskeletal proteins like profilin, DSTN, SPECC1, and DAAM2.
  • However, osteoblasts that differentiated at 42 °C did not organize into a typical whorl agglomerate, even though they appeared to accumulate calcium. This suggests that the cellular structure could be affected under heat stress.
  • The results indicate that the heat stress could have potential long-term impacts on cellular development.

Implications of the Research

  • This research postulates the use of this in vitro stem cell culture model as a helpful tool in investigating the molecular mechanisms affecting cell development in response to stressors. This can have broad implications in understanding how environmental stresses influence cell structure and function.
  • The impact of heat stress on stem cell differentiation can inform medical and scientific approaches towards maintaining cellular health and resilience under varying environmental conditions.

Cite This Article

APA
Abd-El-Aziz A, Riveroll A, Esparza-Gonsalez B, McD￮ L, Cohen AM, Fenech AL, Montelpare WJ. (2022). Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells. Int J Mol Sci, 23(13), 7233. https://doi.org/10.3390/ijms23137233

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 23
Issue: 13
PII: 7233

Researcher Affiliations

Abd-El-Aziz, Ahmad
  • Department of Applied Human Sciences, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Riveroll, Angela
  • Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Esparza-Gonsalez, Blanca
  • Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
McD￮, Laurie
  • Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Cohen, Alejandro M
  • Biological Mass Spectrometry Core Facility, Faculty of Medicine, Dalhousie University, Halifax, NS B3H 0A8, Canada.
Fenech, Adam L
  • School of Climate Change and Adaptation, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
Montelpare, William J
  • Department of Applied Human Sciences, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.

MeSH Terms

  • Animals
  • Heat-Shock Response
  • Horses
  • Mesenchymal Stem Cells / metabolism
  • Proteome / metabolism
  • Proteomics / methods
  • Wnt Signaling Pathway

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Riveroll AL, Skyba-Lewin S, Lynn KD, Mubyeyi G, Abd-El-Aziz A, Kibenge FST, Kibenge MJT, Cohen AM, Esparza-Gonsalez B, McD￮ L, Montelpare WJ. Selection and Validation of Reference Genes for Gene Expression Studies in an Equine Adipose-Derived Mesenchymal Stem Cell Differentiation Model by Proteome Analysis and Reverse-Transcriptase Quantitative Real-Time PCR.. Genes (Basel) 2023 Mar 8;14(3).
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