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Anatomia, histologia, embryologia2020; 50(1); 50-57; doi: 10.1111/ahe.12598

Heat shock protein HSP90 immunoexpression in equine endometrium during oestrus, dioestrus and anoestrus.

Abstract: Heat shock proteins play a crucial role in cellular development, proliferation, differentiation and apoptosis. Heat shock protein 90 (HSP90) has been localised in the human endometrium, where its immunoexpression changes during the menstrual cycle. Similar studies have not been done for the equid species, so the present study aimed to describe endometrial HSP90 immunoexpression in mare endometrium. Endometrial biopsies were formalin-fixed and paraffin-embedded, and sections were stained with haematoxylin-eosin in preparation for HSP90 immunohistochemistry. Immunostaining and morphometric analyses were performed on the epithelial lining, endometrial glands and connective stroma during oestrus, dioestrus phase and anoestrus period (n = 7 per phase or period). Immunoexpression was localised in the basal region of the epithelial cells lining the lumen. Immunoexpression was greater during oestrus than during either dioestrus or anoestrus. During anoestrus, there was little immunostaining in the endometrium, suggesting that HSP90 is involved in the functional modulation of sex steroid receptors in cyclic mares. Indeed, the function of HSP90 as a chaperone in the folding of proteins, such as steroid receptors, might explain the greater intensity of immunostaining during the oestrus and dioestrus phases, compared the anoestrus period. We conclude that, in the mare, HSP90 plays a role in endometrial function and that further studies are needed to test whether it is important in pathological conditions as endometritis.
© 2020 Wiley-VCH GmbH.
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Publication Date: 2020-08-10 PubMed ID: 32776605DOI: 10.1111/ahe.12598Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aims to investigate the behavior of Heat shock protein 90 (HSP90) in the endometrium of mares during different phases of their reproductive cycle, namely oestrus, dioestrus and anoestrus. The findings suggest that HSP90 may be involved in endometrial function and could potentially play a role in pathological conditions like endometritis.

Research Objectives and Methods

  • This study aims to look at the immunoexpression of Heat shock protein 90 (HSP90) in the endometrium of mares during different phases of their reproductive cycle. This is due to analogous observations made in the human endometrium, where HSP90 immunoexpression changes throughout the menstrual cycle.
  • Endometrial biopsies from mares were taken, fixed in formalin and embedded in paraffin, then prepared for HSP90 immunohistochemistry, which is the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues.
  • Immunostaining and morphometric analyses were carried out on various parts of the endometrium: the epithelial lining, endometrial glands, and connective stroma, at each phase of the cycle (oestrus, dioestrus, and anoestrus).

Research Findings

  • The immunoexpression of HSP90 was found in the basal region of the epithelial cells lining the endometrium lumen.
  • The study revealed that immunoexpression was greater during oestrus, compared to either dioestrus or anoestrus. In the anoestrus stage, there was minimal immunostaining in the endometrium. This suggests a functional role of HSP90 in regulating sex steroid receptors in cyclic mares.
  • HSP90’s role as a chaperone protein, implicated in the folding process of other proteins, could explain the more intense immunostaining observed during the oestrus and dioestrus phases, compared to the anoestrus period.

Conclusion and Future Directions

  • The research concluded that HSP90 could be playing a role in endometrial function in mares. However, more research is needed to explore this further.
  • The study also suggests a possible role for HSP90 in endometritis, a pathological condition affecting the endometrium, but this needs further investigation to be confirmed.

Cite This Article

APA
Camacho Benítez A, Vasconcellos R, Lombide P, Viotti H, Pérez W, Cazales N, Cavestany D, Martin GB, Pedrana G. (2020). Heat shock protein HSP90 immunoexpression in equine endometrium during oestrus, dioestrus and anoestrus. Anat Histol Embryol, 50(1), 50-57. https://doi.org/10.1111/ahe.12598

Publication

Anatomia, histologia, embryologia
ISSN: 1439-0264
NlmUniqueID: 7704218
Country: Germany
Language: English
Volume: 50
Issue: 1
Pages: 50-57

Researcher Affiliations

Camacho Benítez, Ana
  • Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Vasconcellos, Rossana
  • Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Lombide, Paula
  • Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Viotti, Helen
  • Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Pérez, William
  • Anatomía, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Cazales, Nicolás
  • Centro de Posgrados, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Cavestany, Daniel
  • Centro de Posgrados, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Martin, Graeme B
  • School of Agriculture and Environment, University of Western Australia, Crawley, WA, Australia.
  • Faculty of Science, UWA School of Agriculture and Environment, University of Western Australia, Perth, Australia.
Pedrana, Graciela
  • Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.

MeSH Terms

  • Anestrus / physiology
  • Animals
  • Diestrus / physiology
  • Endometrium / metabolism
  • Estrus / physiology
  • Female
  • Gene Expression Regulation / drug effects
  • HSP90 Heat-Shock Proteins / genetics
  • HSP90 Heat-Shock Proteins / metabolism
  • Horses / physiology
  • Immunohistochemistry / veterinary

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Citations

This article has been cited 2 times.
  1. Adur MK, Seibert JT, Romoser MR, Bidne KL, Baumgard LH, Keating AF, Ross JW. Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period. J Anim Sci 2022 Jul 1;100(7).
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  2. Lee S, Arffman RK, Komsi EK, Lindgren O, Kemppainen J, Kask K, Saare M, Salumets A, Piltonen TT. Dynamic changes in AI-based analysis of endometrial cellular composition: Analysis of PCOS and RIF endometrium. J Pathol Inform 2024 Dec;15:100364.
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