Heat shock protein HSP90 immunoexpression in equine endometrium during oestrus, dioestrus and anoestrus.
Abstract: Heat shock proteins play a crucial role in cellular development, proliferation, differentiation and apoptosis. Heat shock protein 90 (HSP90) has been localised in the human endometrium, where its immunoexpression changes during the menstrual cycle. Similar studies have not been done for the equid species, so the present study aimed to describe endometrial HSP90 immunoexpression in mare endometrium. Endometrial biopsies were formalin-fixed and paraffin-embedded, and sections were stained with haematoxylin-eosin in preparation for HSP90 immunohistochemistry. Immunostaining and morphometric analyses were performed on the epithelial lining, endometrial glands and connective stroma during oestrus, dioestrus phase and anoestrus period (n = 7 per phase or period). Immunoexpression was localised in the basal region of the epithelial cells lining the lumen. Immunoexpression was greater during oestrus than during either dioestrus or anoestrus. During anoestrus, there was little immunostaining in the endometrium, suggesting that HSP90 is involved in the functional modulation of sex steroid receptors in cyclic mares. Indeed, the function of HSP90 as a chaperone in the folding of proteins, such as steroid receptors, might explain the greater intensity of immunostaining during the oestrus and dioestrus phases, compared the anoestrus period. We conclude that, in the mare, HSP90 plays a role in endometrial function and that further studies are needed to test whether it is important in pathological conditions as endometritis.
© 2020 Wiley-VCH GmbH.
Publication Date: 2020-08-10 PubMed ID: 32776605DOI: 10.1111/ahe.12598Google Scholar: Lookup
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- Journal Article
Summary
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The study aims to investigate the behavior of Heat shock protein 90 (HSP90) in the endometrium of mares during different phases of their reproductive cycle, namely oestrus, dioestrus and anoestrus. The findings suggest that HSP90 may be involved in endometrial function and could potentially play a role in pathological conditions like endometritis.
Research Objectives and Methods
- This study aims to look at the immunoexpression of Heat shock protein 90 (HSP90) in the endometrium of mares during different phases of their reproductive cycle. This is due to analogous observations made in the human endometrium, where HSP90 immunoexpression changes throughout the menstrual cycle.
- Endometrial biopsies from mares were taken, fixed in formalin and embedded in paraffin, then prepared for HSP90 immunohistochemistry, which is the process of localizing proteins in cells of a tissue section exploiting the principle of antibodies binding specifically to antigens in biological tissues.
- Immunostaining and morphometric analyses were carried out on various parts of the endometrium: the epithelial lining, endometrial glands, and connective stroma, at each phase of the cycle (oestrus, dioestrus, and anoestrus).
Research Findings
- The immunoexpression of HSP90 was found in the basal region of the epithelial cells lining the endometrium lumen.
- The study revealed that immunoexpression was greater during oestrus, compared to either dioestrus or anoestrus. In the anoestrus stage, there was minimal immunostaining in the endometrium. This suggests a functional role of HSP90 in regulating sex steroid receptors in cyclic mares.
- HSP90’s role as a chaperone protein, implicated in the folding process of other proteins, could explain the more intense immunostaining observed during the oestrus and dioestrus phases, compared to the anoestrus period.
Conclusion and Future Directions
- The research concluded that HSP90 could be playing a role in endometrial function in mares. However, more research is needed to explore this further.
- The study also suggests a possible role for HSP90 in endometritis, a pathological condition affecting the endometrium, but this needs further investigation to be confirmed.
Cite This Article
APA
Camacho Benítez A, Vasconcellos R, Lombide P, Viotti H, Pérez W, Cazales N, Cavestany D, Martin GB, Pedrana G.
(2020).
Heat shock protein HSP90 immunoexpression in equine endometrium during oestrus, dioestrus and anoestrus.
Anat Histol Embryol, 50(1), 50-57.
https://doi.org/10.1111/ahe.12598 Publication
Researcher Affiliations
- Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- Anatomía, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- Centro de Posgrados, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- Centro de Posgrados, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
- School of Agriculture and Environment, University of Western Australia, Crawley, WA, Australia.
- Faculty of Science, UWA School of Agriculture and Environment, University of Western Australia, Perth, Australia.
- Histología y Embriología, Biociencias, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
MeSH Terms
- Anestrus / physiology
- Animals
- Diestrus / physiology
- Endometrium / metabolism
- Estrus / physiology
- Female
- Gene Expression Regulation / drug effects
- HSP90 Heat-Shock Proteins / genetics
- HSP90 Heat-Shock Proteins / metabolism
- Horses / physiology
- Immunohistochemistry / veterinary
References
This article includes 24 references
- Avenatti RC, McKeever KH, Horohov DW, Malinowski K. Effects of age and exercise on inflammatory cytokines, HSP70 and HSP90 gene expression and protein content in Standardbred horses. Comparative Exercise Physiology 14(1), 27-46.
- Barone, R. (1978). Anatomie comparée des mammifères domestiques, 1st ed. Lyon, France: Beaux-Arts J. Tixier & Fils. S.A.
- Blesson CS, Sahlin L. Prostaglandin E and F receptors in the uterus. Receptors & Clinical Investigation 2(1), 127-128.
- César Cortés-González C, Ramírez-González V, Ariza AC, Bobadilla NA. Significado funcional de las proteínas de choque térmico de 90 kDa. Revista de Investigación Clínica 60(4), 311-320.
- Coleman SJ, Zeng Z, Hestand MS, Liu J, Macleod JN. Analysis of unannotated equine transcripts identified by mRNA sequencing. PLoS One 8(7), e70125.
- De Maio A, Gabriella Santoro M, Tanguay RM, Hightower LE. Ferruccio Ritossa's scientific legacy 50 years after his discovery of the heat shock response: A new view of biology, a new society, and a new journal. Cell Stress and Chaperones 17(9), 139-143.
- Hanada M, Maeda Y, Oikawa M. Equine endometrial gland density and endometrial thickness vary among sampling sites in thoroughbred mares. Journal of Equine Science 23(3), 35-40.
- Hartt LS, Carling SJ, Joyce MM, Johnson GA, Vanderwall DK, Ott TL. Temporal and spatial associations of oestrogen receptor alpha and progesterone receptor in the endometrium of cyclic and early pregnant mares. Reproduction 130(2), 241-250.
- Kampinga HH, Hageman J, Vos MJ, Kubota H, Tanguay RM, Bruford EA, Hightower LE. Guidelines for the nomenclature of the human heat shock proteins. Cell Stress and Chaperones 14(1), 105-111.
- Kenney RM. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death. Journal of the American Veterinary Medical Association 172(3), 241-262.
- Komatsu T. Messenger ribonucleic acid expression of heat shock proteins HSP70 and HSP90 in human endometrium and myometrium during the menstrual cycle. Journal of Clinical Endocrinology & Metabolism 82(5), 1385-1389.
- Koshiyama M, Konishi I, Nanbu K, Nanbu Y, Mandai M, Komatsu T, Fujii S. Immunohistochemical localization of heat shock proteins HSP70 and HSP90 in the human endometrium: Correlation with sex steroid receptors and Ki-67 antigen expression. Journal of Clinical Endocrinology and Metabolism 80(4), 1106-1112.
- Marth CD, Young ND, Glenton LY, Noden DM, Browning GF, Krekeler N. Deep sequencing of the uterine immune response to bacteria during the equine oestrous cycle. BMC Genomics 16(1), 934.
- Pennisi R, Ascenzi P, di Masi A. Hsp90: A new player in DNA repair?. Biomolecules 5(4), 2589-2618.
- Peterson C, Driskell E, Wilkie D, Premanandan C, Hamor R. Heat-shock protein 70 expression in the equine cornea. Veterinary Ophthalmology 20(4), 344-348.
- Pires ES, Choudhury AK, Idicula-Thomas S, Khole VV. Anti-HSP90 autoantibodies in sera of infertile women identify a dominant, conserved epitope EP6 (380-389) of HSP90 beta protein. Reproductive Biology and Endocrinology : RB&E 9, 16.
- Pratt WB. The role of thehsp90-based chaperone system in signal transduction by nuclear receptors and receptors signaling via map kinase. Annual Review of Pharmacology and Toxicology 37(1), 297-326.
- Röhl A, Rohrberg J, Buchner J. The chaperone Hsp90: Changing partners for demanding clients. Trends in Biochemical Sciences 38(5), 253-262.
- Saibil H. Chaperone machines for protein folding, unfolding and disaggregation. Nature Reviews Molecular Cell Biology 14(10), 630-642.
- Sunil Kumar BV, Bhardwaj R, Mahajan K, Kashyap N, Kumar A, Verma R. The overexpression of Hsp90B1 is associated with tumorigenesis of canine mammary glands. Molecular and Cellular Biochemistry 440(1-2), 23-31.
- Tabibzadeh S, Broome J. Heat shock proteins in human endometrium throughout the menstrual cycle. Infectious Diseases in Obstetrics and Gynecology 7(1-2), 5-9.
- Tanioka T, Nakatani Y, Kobayashi T, Tsujimoto M, Oh-ishi S, Murakami M, Kudo I. Regulation of cytosolic prostaglandin E2 synthase by 90-kDa heat shock protein. Biochemical and Biophysical Research Communications 303(4), 1018-1023.
- Wataba K, Saito T, Fukunaka K, Ashihara K, Nishimura M, Kudo R. Over-expression of heat shock proteins in carcinogenic endometrium. International Journal of Cancer 91(4), 448-456.
- Yuan J-X, Xiao L-J, Lu C-L, Zhang X-S, Liu T, Chen M, Liu Y-X. Increased expression of heat shock protein 105 in rat uterus of early pregnancy and its significance in embryo implantation. Reproductive Biology and Endocrinology 7(1), 23.
Citations
This article has been cited 1 times.- Adur MK, Seibert JT, Romoser MR, Bidne KL, Baumgard LH, Keating AF, Ross JW. Porcine endometrial heat shock proteins are differentially influenced by pregnancy status, heat stress, and altrenogest supplementation during the peri-implantation period.. J Anim Sci 2022 Jul 1;100(7).
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