Heterotopic transfer of fresh and cryopreserved autogenous articular cartilage in the horse.
Abstract: Two 10 mm thick osteochondral grafts were harvested from the lateral aspect of the lateral trochlear ridge of the left talus in each of 10 anesthetized horses. The grafts were frozen in a 7.5% DMSO solution and stored in liquid nitrogen. The horses were anesthetized again on day 14 and the thawed grafts were press-fitted into drill holes in the trochlear ridges of the right stifle. A fresh graft was transferred from the right hock to the left stifle. To control for the effects of surgery, another fresh graft was transferred from the right stifle to the left stifle. The result was two grafts in each femoropatellar joint. Fresh and frozen osteoarticular autografts appeared to maintain a durable weight-bearing surface for 3 months; however, the fresh grafts were clearly superior. Frozen grafts had fewer living chondrocytes, decreased safranin-O staining, and decreased SO435 uptake. Graft stability and articular surface congruency were determining factors in the outcome of all grafts. Since the availability of osteochondral autografts is limited, further work on the use of preserved allogeneic osteochondral tissue is warranted.
Publication Date: 1991-11-01 PubMed ID: 1369528DOI: 10.1111/j.1532-950x.1991.tb00352.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper focusses on a study in which fresh and frozen autogenous articular cartilage were transferred in horses. The results indicated that fresh grafts performed better, although both types appeared to maintain weight-bearing capacity for a short-term period.
Experimental Procedure
- The study was conducted on 10 anesthetized horses from which two 10 mm thick osteochondral grafts were harvested.
- The chosen location for graft extraction was the lateral aspect of the lateral trochlear ridge of the left talus.
- These harvested grafts were first frozen using a 7.5% DMSO solution and then stored in liquid nitrogen.
- Fourteen days later, the horses were anesthetized again and these thawed grafts were inserted into drill holes in the trochlear ridges of the right stifle.
- To examine the difference in outcomes between fresh and frozen grafts, a fresh graft was transferred from the right hock to the left stifle. As a control measure, another fresh graft was transferred from the right stifle to the left stifle.
- The procedure resulted in each femoropatellar joint of the subject horses having two grafts.
Results and Findings
- The fresh and frozen autografts were able to maintain a weight-bearing surface for 3 months, with results indicating the fresh grafts as clearly superior.
- Compared to fresh ones, the frozen grafts had fewer living chondrocytes, decreased safranin-O staining, and decreased SO435 uptake.
- The outcomes of all the grafts were found to be determined by graft stability and the congruency of the articular surface.
Conclusion and Future Considerations
- In conclusion, the study indicated that while both fresh and frozen autografts could maintain a weight-bearing surface short-term, the fresh grafts were superior in their performance.
- Given the limited availability of osteochondral autografts, the study suggests the need for further research on the use of preserved allogeneic osteochondral tissue.
Cite This Article
APA
Desjardins MR, Hurtig MB, Palmer NC.
(1991).
Heterotopic transfer of fresh and cryopreserved autogenous articular cartilage in the horse.
Vet Surg, 20(6), 434-445.
https://doi.org/10.1111/j.1532-950x.1991.tb00352.x Publication
Researcher Affiliations
- Department of Clinical Studies, University of Guelph, Canada.
MeSH Terms
- Animals
- Cartilage, Articular / pathology
- Cartilage, Articular / transplantation
- Cryopreservation / veterinary
- Horses / surgery
- Stifle / pathology
- Stifle / surgery
- Talus / pathology
- Talus / surgery
- Transplantation, Autologous / veterinary
- Transplantation, Heterotopic / veterinary
Citations
This article has been cited 2 times.- Waselau AC, Nadler D, Müller JM, Zlinszky K, Hilbe M, Auer JA, von Rechenberg B. The effect of cartilage and bone density of mushroom-shaped, photooxidized, osteochondral transplants: an experimental study on graft performance in sheep using transplants originating from different species. BMC Musculoskelet Disord 2005 Dec 15;6:60.
- Haisch A, Wanjura F, Radke C, Leder-Jöhrens K, Gröger A, Endres M, Klaering S, Loch A, Sittinger M. Immunomodulation of tissue-engineered transplants: in vivo bone generation from methylprednisolone-stimulated chondrocytes. Eur Arch Otorhinolaryngol 2004 Apr;261(4):216-24.
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