HI1 and I1 Resistance Plasmids from Salmonella enterica Serovar Typhimurium Strain SRC27 Are Epidemic.
Abstract: Conjugative plasmids are a major contributor to the global spread of antibiotic resistance determinants, but the tracking of their evolutionary history is often neglected. serovar Typhimurium ( Typhimurium) strain SRC27 was isolated from an equine infection in Australia in 1999. SRC27 was known to carry conjugative HI1 and I1 resistance plasmids. In this study, SRC27 was sequenced to determine the relationship between these HI1 and I1 resistance plasmids it was known to carry and HI1 and I1 resistance plasmids circulating worldwide. The resistance genes in the HI1 plasmid, pSRC27-H, are all located in a single complex 34.7 kb resistance region. The backbone sequence and location of the pSRC27-H resistance island were used to identify the most closely related HI1 plasmids among the >90 that have been sequenced since 2011. This defined a sublineage of 20 type 2 HI1 plasmids that have been circulating in Europe, Asia, North America, and Australia since at least 1993. The overall resistance gene content of these HI1 plasmids differs, indicating extensive evolution through the acquisition of additional transposons and deletion or replacement of ancestral regions. The I1 plasmid contains a complete copy of Tna, containing the genes that confer resistance to streptomycin. The precise location of Tna in the backbone also defined a globally disseminated sublineage of I1 plasmids, many of which have also acquired additional resistance determinants. The sequence revealed that SRC27 also carried two additional plasmids, the pSLT-type FIB(S):FII(S) virulence plasmid and a small cryptic theta-replicating Col156 plasmid.
Publication Date: 2021-07-08 PubMed ID: 34242087DOI: 10.1089/mdr.2020.0579Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the global distribution and evolution of HI1 and I1 antibiotic resistance plasmids in the Salmonella enterica Typhimurium strain SRC27, which was first identified in Australia in 1999.
Understanding the Research Background
- Salmonella enterica Typhimurium strain SRC27 was known to carry HI1 and I1 resistance plasmids, which are types of genes that contribute to antibiotic resistance.
- The study was conducted as a way of understanding the connection between these HI1 and I1 resistance plasmids and similar ones found across the globe.
- The global spread of antibiotic resistance, largely facilitated by such plasmids, is a significant health concern.
Research Process and Key Findings
- The study involved sequencing SRC27 to understand the relationship between the HI1 and I1 resistance plasmids it carries, and similar plasmids found around the world.
- This study identified a closely related group of 20 type 2 HI1 plasmids, distinguished by the backbone sequence and location of the resistance genes.
- These related plasmids have been circulating globally since 1993.
- Despite their relationship, these HI1 plasmids possess different resistance gene content, suggesting they have evolved over time through the acquisition of different transposons, deletion, or replacement of their original components.
- The I1 plasmid contained a streptomycin resistance gene, defining a related global dynamic group of I1 plasmids, many of which have gained further resistance elements.
- Additionally, the SRC27 strain was discovered to contain two more plasmids, contributing to its virulence and cryptic theta replication.
Implications of the Study
- The study provides a better understanding of the evolution and widespread occurence of HI1 and I1 antibiotic resistance plasmids and their role in contributing to global antibiotic resistance.
- This knowledge could assist in tracking the spread and development of antibiotic resistance, ultimately aiding in the creation of strategies to mitigate this global health issue.
Cite This Article
APA
Harmer CJ.
(2021).
HI1 and I1 Resistance Plasmids from Salmonella enterica Serovar Typhimurium Strain SRC27 Are Epidemic.
Microb Drug Resist, 27(11), 1495-1504.
https://doi.org/10.1089/mdr.2020.0579 Publication
Researcher Affiliations
- School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia.
MeSH Terms
- Animals
- Drug Resistance, Multiple, Bacterial / genetics
- Genes, Bacterial
- Horse Diseases / microbiology
- Horses
- Microbial Sensitivity Tests
- Plasmids / genetics
- Salmonella enterica / genetics
- Salmonella typhimurium / genetics
Citations
This article has been cited 4 times.- Verhaegen M, Bergot T, Liebana E, Stancanelli G, Streissl F, Mingeot-Leclercq MP, Mahillon J, Bragard C. On the use of antibiotics to control plant pathogenic bacteria: a genetic and genomic perspective. Front Microbiol 2023;14:1221478.
- Snaith AE, Dunn SJ, Moran RA, Newton PN, Dance DAB, Davong V, Kuenzli E, Kantele A, Corander J, McNally A. The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage. Microb Genom 2023 May;9(5).
- Harmer CJ, Lebreton F, Stam J, McGann PT, Hall RM. Complete genome of the extensively antibiotic-resistant GC1 Acinetobacter baumannii isolate MRSN 56 reveals a novel route to fluoroquinolone resistance. J Antimicrob Chemother 2022 Jun 29;77(7):1851-1855.
- Martino F, Petroni A, Menocal MA, Corso A, Melano R, Faccone D. New insights on mcr-1-harboring plasmids from human clinical Escherichia coli isolates. PLoS One 2024;19(2):e0294820.
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