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Home / Equine Research Bank / Vet Ophthalmol / Histologic effects of MicroPulse™ transscleral cyclop...
Veterinary ophthalmology2020; 24(1); 59-70; doi: 10.1111/vop.12846

Histologic effects of MicroPulse™ transscleral cyclophotocoagulation in normal equine eyes.

Abstract: Determine the immediate post-operative effects of MicroPulse transscleral cyclophotocoagulation (MP-TSCPC) in healthy equine eyes. Methods: Ten adult horses. Methods: MP-TSCPC was performed on sedated horses in 12 eyes (4 groups) using the following parameters (power, duration, duty cycle): (1) 2000 mW, 180 seconds, 31.3%; (2) 3000 mW, 180 seconds, 31.3%; (3) 3000 mW, 270 seconds, 31.3%; and (4) 3000 mW, 270 seconds, 50%. Three additional eyes were left untreated (control). Eyes were monitored clinically until euthanasia (mean = 3 hours post-procedure). Histologic sections were assessed with light microscopy and transmission electron microscopy (TEM). Results: MP-TSCPC was well tolerated by sedated horses. Adverse effects were only noted in Group 4: ocular hypertension (n = 3/3), conjunctival burns (3/3), aqueous flare (2/3), and a corneal erosion (1/3). Histologic scoring of Group 4 was statistically greater than other treated groups (1-3) and control eyes (P ≤ .021). TEM showed subtle changes to the mitochondria and plasma membrane infoldings of the basilar surface of the nonpigmented epithelium in all treated eyes. Conclusions: MP-TSCPC does not cause immediate post-procedure adverse clinical effects or pronounced morphological changes to the ciliary body, except with the highest laser settings evaluated (power 3000 mW, duration 270 seconds, duty cycle 50%).
© 2020 American College of Veterinary Ophthalmologists.
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Publication Date: 2020-11-29 PubMed ID: 33249770DOI: 10.1111/vop.12846Google Scholar: Lookup
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  • Clinical Trial
  • Veterinary
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focused on understanding the immediate effect of MicroPulse transscleral cyclophotocoagulation (MP-TSCPC) on healthy horse eyes, gathering data from various procedures and comparing their results.

Methodology

  • The researchers conducted MP-TSCPC on the eyes of adult horses. The horses were sedated during the experiment. This process was performed on a total of 12 eyes, which were divided into four groups. Each group had different settings for power, duration, and duty cycle.
  • For control, an additional three eyes were left untreated. The eyes were clinically monitored until the horses’ euthanasia, which was on average three hours post-procedure.
  • The researchers used light microscopy and transmission electron microscopy (TEM) to assess the histologic sections.

Results

  • The results showed that MP-TSCPC was well tolerated by the sedated horses. Any adverse effects were noted only in Group 4, the group with the highest laser settings (power of 3000 mW, duration of 270 seconds, and a duty cycle of 50%). This included ocular hypertension, conjunctival burns, aqueous flare, and corneal erosion.
  • The histologic scoring for Group 4 was statistically higher than for the other groups and the untreated eyes (control).
  • Transmission electron microscopy (TEM) revealed mild changes to the mitochondria and plasma membrane infoldings of the basilar surface of the nonpigmented epithelium in all treated eyes.

Conclusions

  • The researchers concluded that MP-TSCPC did not cause immediate adverse clinical effects or intense morphological changes to the ciliary body, except when exposed to the highest laser settings (power 3000 mW, duration 270 seconds, duty cycle 50%).

Cite This Article

APA
Foote BC, Smith JD, Allbaugh RA, Sebbag L. (2020). Histologic effects of MicroPulse™ transscleral cyclophotocoagulation in normal equine eyes. Vet Ophthalmol, 24(1), 59-70. https://doi.org/10.1111/vop.12846

Publication

Veterinary ophthalmology
ISSN: 1463-5224
NlmUniqueID: 100887377
Country: England
Language: English
Volume: 24
Issue: 1
Pages: 59-70

Researcher Affiliations

Foote, Braidee C
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Smith, Jodi D
  • Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Allbaugh, Rachel A
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
Sebbag, Lionel
  • Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

MeSH Terms

  • Animals
  • Ciliary Body / anatomy & histology
  • Ciliary Body / surgery
  • Ciliary Body / ultrastructure
  • Eye / anatomy & histology
  • Eye / ultrastructure
  • Female
  • Horses / surgery
  • Laser Coagulation / methods
  • Laser Coagulation / veterinary
  • Male
  • Postoperative Period

Grant Funding

  • VAF2018-01 / ACVO Vision for Animals Foundation

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Citations

This article has been cited 4 times.
  1. Nassiri N, Tseng VL, Kim C, Dentone P, Francis NM, Chopra AL, Huang A, Francis BA. Outcomes of microPulse transscleral laser therapy in eyes with prior glaucoma aqueous tube shunt. Graefes Arch Clin Exp Ophthalmol 2023 May 29;.
    doi: 10.1007/s00417-023-06119-9pubmed: 37247002google scholar: lookup
  2. Bernardi E, Töteberg-Harms M. MicroPulse Transscleral Laser Therapy Demonstrates Similar Efficacy with a Superior and More Favorable Safety Profile Compared to Continuous-Wave Transscleral Cyclophotocoagulation. J Ophthalmol 2022;2022:8566044.
    doi: 10.1155/2022/8566044pubmed: 35178250google scholar: lookup
  3. Phanvichatkul N, Suvannachart P. Intraocular pressure changes and pain scores within 24 hours and short-term outcomes after micropulse transscleral laser therapy. PLoS One 2026;21(2):e0340625.
    doi: 10.1371/journal.pone.0340625pubmed: 41650162google scholar: lookup
  4. Hallaj S, Pashaee B, Nieves CJ, Shah SJ, Ezeonu S, Xia T, Garg A, Sheng Y, Dale EA, Schmidt C, Shukla AG, Pro MJ, Kolomeyer NN, Lee D, Moster MR, Myers JS, Razeghinejad R. Comparison of outcomes of continuous-wave and micropulse trans-scleral cyclophotocoagulation: A retrospective cohort study. Eur J Ophthalmol 2025 Sep;35(5):1647-1653.
    doi: 10.1177/11206721251332674pubmed: 40232272google scholar: lookup
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