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Home / Equine Research Bank / Anim Reprod / Histomorphometric and vascular changes in equine endometrium...
Animal reproduction2020; 17(2); e20200006; doi: 10.1590/1984-3143-AR2020-0006

Histomorphometric and vascular changes in equine endometrium after the infusion of conceptus fragments.

Abstract: This experiment aimed to verify if the proteins present in a 13 day conceptus induce changes in the equine endometrial ultra-structure, histology, and vascularization, two days after its infusion. Ten healthy cyclic mares were used. Once estrus was confirmed, mares were examined daily to detect ovulation (day 0). After ovulation, mares were examined daily until day seven by transrectal palpation and B-mode and Doppler ultrasonography. In this first cycle, intrauterine biopsies were collected at day seven after ovulation, constituting the Cyclic group (n = 10). In the second cycle, the same mares daily were examined until ovulation was detected. After ovulation, mares were examined daily by transrectal palpation and B-mode and Doppler ultrasonography until day 7. On day 5, after ovulation, fragments from previously collected 13-day-old concepti were infused into the uterus of each mare. Intrauterine biopsies were collected at day 7 in all mares (n = 10), constituting the Fragment group. The percentage of ciliated and flattened cells decreased in the Fragment group. Protruded cells, superficial and intraglandular secretion, glandular lumen and diameter, blood vessel diameter, endometrial vascularization, and immune cells were higher in the Fragment group than in the Cyclic group. In summary, proteins of 13 day equine conceptus fragments infused at day five after ovulation signaled histological and vascular changes in the endometrium at the 7 day after ovulation.
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Publication Date: 2020-06-29 PubMed ID: 32714458PubMed Central: PMC7375867DOI: 10.1590/1984-3143-AR2020-0006Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research evaluates the impact of proteins found in 13-day old equine conceptus on the structure and blood vessel development of equine endometrium, two days after being infused.

Objective of the Study

  • The main aim of this study was to examine whether proteins from a 13-day old conceptus could induce structural, histological, and vascular changes in equine endometrium.

Methodology

  • The study used ten healthy, cyclic mares. They were examined daily after the confirmation of estrus to detect ovulation.
  • In the first cycle, intrauterine biopsies were taken at day seven post-ovulation, forming the Cyclic group.
  • In the subsequent cycle, the mares were inspected daily until ovulation was recognized. Fragments from previously collected 13-day-old concepti were infused into each mare’s uterus on day 5.
  • Intrauterine biopsies were again collected in all mares at day 7, forming the Fragment group.

Results

  • There was a decrease in the percentage of ciliated and flattened cells in the Fragment group as compared to the Cyclic group.
  • The Fragment group showed higher quantities of protruded cells, superficial and intraglandular secretion, larger glandular lumen and diameter, irrease in blood vessel diameter, greater endometrial vascularization, and more immune cells.

Conclusion

  • The results of the study concluded that proteins from 13-day old equine conceptus fragments, when infused into the uterus at day five post-ovulation, induces histological and vascular changes in the endometrium at day seven post-ovulation.

Cite This Article

APA
Camacho CA, Estradé MJ, Cazales N, Caballeros JE, Fiala-Rechsteiner SM, Neves AP, Mattos RC. (2020). Histomorphometric and vascular changes in equine endometrium after the infusion of conceptus fragments. Anim Reprod, 17(2), e20200006. https://doi.org/10.1590/1984-3143-AR2020-0006

Publication

Animal reproduction
ISSN: 1984-3143
NlmUniqueID: 101272804
Country: Brazil
Language: English
Volume: 17
Issue: 2
Pages: e20200006
PII: e20200006

Researcher Affiliations

Camacho, Cesar Augusto
  • Laboratório de Reprodução Animal, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Estradé, Maria José
  • Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Cazales, Nicolás
  • Laboratório de Reprodução Animal, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
  • Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Caballeros, Jorge Emilio
  • Laboratório de Reprodução Animal, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Fiala-Rechsteiner, Sandra Mara
  • Historep, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas, RS, Brasil.
Neves, Adriana Pires
  • Universidade Federal do Pampa, Dom Pedrito, RS, Brasil.
Mattos, Rodrigo Costa
  • Laboratório de Reprodução Animal, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.

Conflict of Interest Statement

Conflicts of interest: The authors have no conflict of interest to declare.

References

This article includes 43 references
  1. Aurich C, Budik S. Early pregnancy in the horse revisited - does exception prove the rule?. J Anim Sci Biotechnol 2015;6:50.
    doi: 10.1186/s40104-015-0048-6pmc: PMC4668677pubmed: 26635959google scholar: lookup
  2. Ball B, Altschul M, Hillman R. Luteal maintenance in mares after transfer of equine trophoblastic vesicles.. Equine Vet J 1989;21(S8):21–24.
    doi: 10.1111/j.2042-3306.1989.tb04667.xgoogle scholar: lookup
  3. Bastos HBA, Martinez MN, Camozzato GC, Estradé MJ, Barros E, Vital CE, Vidigal PMP, Meikle A, Jobim MIM, Gregory RM, Mattos RC. Proteomic profile of histotroph during early embryo development in mares.. Theriogenology 2019 Feb;125:224-235.
    doi: 10.1016/j.theriogenology.2018.11.002pubmed: 30472502google scholar: lookup
  4. Bazer FW, Roberts RM, Thatcher WW. Actions of hormones on the uterus and effect on conceptus development.. J Anim Sci 1979;49 Suppl 2:35-45.
    doi: 10.1093/ansci/49.Supplement_II.35pubmed: 400776google scholar: lookup
  5. Bazer FW, Vallet JL, Roberts RM, Sharp DC, Thatcher WW. Role of conceptus secretory products in establishment of pregnancy.. J Reprod Fertil 1986 Mar;76(2):841-50.
    doi: 10.1530/jrf.0.0760841pubmed: 3517318google scholar: lookup
  6. Bollwein H, Maierl J, Mayer R, Stolla R. Transrectal color Doppler sonography of the A. uterina in cyclic mares.. Theriogenology 1998 Jun;49(8):1483-8.
    doi: 10.1016/S0093-691X(98)00094-6pubmed: 10732012google scholar: lookup
  7. Bollwein H, Mayer R, Stolla R. Transrectal Doppler sonography of uterine blood flow during early pregnancy in mares.. Theriogenology 2003 Sep 1;60(4):597-605.
    doi: 10.1016/S0093-691X(03)00080-3pubmed: 12832010google scholar: lookup
  8. Brady HA, Burghardt RC, Evans JW, Blanchard TL, Vamer DD, Bruemmer JE. Model system for the study of uterine/trophoblast interactions in the mare.. J Equine Vet Sci 1993;13(9):506–511.
    doi: 10.1016/S0737-0806(07)80265-6google scholar: lookup
  9. Caballeros JE, Camacho C, Cazales N, Estradé MJ, Fiala-Rechsteiner S, Jobim MIM, Mattos RC. Ultrastructural and histological characteristics of the equine endometrium at day 5 post ovulation.. Theriogenology 2019 Jul 1;132:106-112.
    doi: 10.1016/j.theriogenology.2019.04.006pubmed: 31004876google scholar: lookup
  10. Camous S, Heyman Y, Méziou W, Ménézo Y. Cleavage beyond the block stage and survival after transfer of early bovine embryos cultured with trophoblastic vesicles.. J Reprod Fertil 1984 Nov;72(2):479-85.
    doi: 10.1530/jrf.0.0720479pubmed: 6512770google scholar: lookup
  11. Camozzato GC, Martinez MN, Bastos HBA, Fiala-Rechsteiner S, Meikle A, Jobim MIM, Gregory RM, Mattos RC. Ultrastructural and histological characteristics of the endometrium during early embryo development in mares.. Theriogenology 2019 Jan 1;123:1-10.
    doi: 10.1016/j.theriogenology.2018.09.018pubmed: 30253251google scholar: lookup
  12. Cuervo-Arango J, Claes AN, Stout TA. Effect of embryo transfer technique on the likelihood of pregnancy in the mare: a comparison of conventional and Wilsher's forceps-assisted transfer.. Vet Rec 2018 Sep 15;183(10):323.
    doi: 10.1136/vr.104808pubmed: 29798842google scholar: lookup
  13. Das SK. Cell cycle regulatory control for uterine stromal cell decidualization in implantation.. Reproduction 2009 Jun;137(6):889-99.
    doi: 10.1530/REP-08-0539pubmed: 19307426google scholar: lookup
  14. Fahy GM, Wowk B. Principles of cryopreservation by vitrification.. Methods Mol Biol 2015;1257:21-82.
    doi: 10.1007/978-1-4939-2193-5_2pubmed: 25428002google scholar: lookup
  15. Garber Cohen IP, Castello PR, González Flecha FL. Ice-induced partial unfolding and aggregation of an integral membrane protein.. Biochim Biophys Acta 2010 Nov;1798(11):2040-7.
    doi: 10.1016/j.bbamem.2010.07.035pubmed: 20691147google scholar: lookup
  16. Ginther OJ. Ultrasonic imaging and animal reproduction: book 4, color-Doppler ultrasonography.. Cross Plains, WI: Equiservices Publishing; 2007. 258.
  17. Heyman Y, Chesné P, Chupin D, Ménézo Y. Improvement of survival rate of frozen cattle blastocysts after transfer with trophoblastic vesicles.. Theriogenology 1987 Mar;27(3):477-84.
    doi: 10.1016/0093-691X(87)90235-4pubmed: 16726252google scholar: lookup
  18. Keenan LR, Forde D, McGeady T, Wade J, Roche JF. Endometrial histology of early pregnant and non-pregnant mares.. J Reprod Fertil Suppl 1987;35:499-504.
    doi: 10.1063/1.857816pubmed: 3479603google scholar: lookup
  19. Kenney RM, Doig PA. Equine endometrial biopsy.. In: Morrow DA, editor. Current therapy in theriogenology. Philadelphia: W.B. Saunders; 1986. pp. 723–729.
  20. Klein C, Troedsson MH. Maternal recognition of pregnancy in the horse: a mystery still to be solved.. Reprod Fertil Dev 2011;23(8):952-63.
    doi: 10.1071/RD10294pubmed: 22127001google scholar: lookup
  21. Klein C, Troedsson MH. Transcriptional profiling of equine conceptuses reveals new aspects of embryo-maternal communication in the horse.. Biol Reprod 2011 May;84(5):872-85.
    doi: 10.1095/biolreprod.110.088732pubmed: 21209420google scholar: lookup
  22. Klein C. Early pregnancy in the mare: old concepts revisited.. Domest Anim Endocrinol 2016 Jul;56 Suppl:S212-7.
    doi: 10.1016/j.domaniend.2016.03.006pubmed: 27345319google scholar: lookup
  23. Malschitzky E, Schilela A, Meirelles LS, Gelpi Mattos AL, Gregory RM, Mattos RC. Artificial photoperiod in pregnant mares and its effect on pregnancy length and postpartum reproductive performance.. Pferdeheilkunde 2001;17(6):565–569.
    doi: 10.21836/PEM20010605google scholar: lookup
  24. Martínez Pereira MN. Preñez temprana en la yegua: cambios histomorfológicos e inflamatorios endometriales y de hormonas circulantes desde la ovulación hasta los 13 días.. Montevideo: Universidad de la República; 2016. thesis.
  25. McDowell KJ, Sharp DC, Fazleabas AT, Roberts RM. Two-dimensional polyacrylamide gel electrophoresis of proteins synthesized and released by conceptuses and endometria from pony mares.. J Reprod Fertil 1990 May;89(1):107-15.
    doi: 10.1530/jrf.0.0890107pubmed: 2374112google scholar: lookup
  26. Merkl M, Ulbrich SE, Otzdorff C, Herbach N, Wanke R, Wolf E, Handler J, Bauersachs S. Microarray analysis of equine endometrium at days 8 and 12 of pregnancy.. Biol Reprod 2010 Nov;83(5):874-86.
    doi: 10.1095/biolreprod.110.085233pubmed: 20631402google scholar: lookup
  27. Nadkarni S, Smith J, Sferruzzi-Perri AN, Ledwozyw A, Kishore M, Haas R, Mauro C, Williams DJ, Farsky SH, Marelli-Berg FM, Perretti M. Neutrophils induce proangiogenic T cells with a regulatory phenotype in pregnancy.. Proc Natl Acad Sci U S A 2016 Dec 27;113(52):E8415-E8424.
    doi: 10.1073/pnas.1611944114pmc: PMC5206541pubmed: 27956610google scholar: lookup
  28. Nie GJ, Johnson KE, Braden TD, Wenzel JGW. Use of an intra-uterine glass ball protocol to extend luteal function in mares.. J Equine Vet Sci 2003;23(6):266–273.
    doi: 10.1053/jevs.2003.75google scholar: lookup
  29. Nieto-Olmedo P, Tapia JA, Peña FJ, Ferrusola CO. The use of power mode doppler ultrasonography as a predictive tool of early pregnancy in the mare.. J Equine Vet Sci 2018;66:218–219.
    doi: 10.1016/j.jevs.2018.05.106google scholar: lookup
  30. Pearson H. Reproductive immunology: Immunity's pregnant pause.. Nature 2002 Nov 21;420(6913):265-6.
    doi: 10.1038/420265apubmed: 12447413google scholar: lookup
  31. Ricketts SW, Rossdale PD, Samuel CA. Endometrial biopsy studies of mares with contagious equine metritis 1977.. Equine Vet J 1978 Jul;10(3):160-6.
    doi: 10.1111/j.2042-3306.1978.tb02247.xpubmed: 688997google scholar: lookup
  32. Rivera Del Alamo MM, Reilas T, Kindahl H, Katila T. Mechanisms behind intrauterine device-induced luteal persistence in mares.. Anim Reprod Sci 2008 Aug;107(1-2):94-106.
    doi: 10.1016/j.anireprosci.2007.06.010pubmed: 17643876google scholar: lookup
  33. Rowson LE, Moor RM. The influence of embryonic tissue homogenate infused into the uterus, on the life-span of the corpus luteum in the sheep.. J Reprod Fertil 1967 Jun;13(3):511-6.
    doi: 10.1530/jrf.0.0130511pubmed: 6067575google scholar: lookup
  34. Samuel CA, Ricketts SW, Rossdale PD, Steven DH, Thurley KW. Scanning electron microscope studies of the endometrium of the cyclic mare.. J Reprod Fertil Suppl 1979;(27):287-92.
    pubmed: 289802
  35. Sharp DC, McDowell KJ, Weithenauer J, Thatcher WW. The continuum of events leading to maternal recognition of pregnancy in mares.. J Reprod Fertil Suppl 1989;37:101-7.
    pubmed: 2810225
  36. Silva LA, Gastal EL, Beg MA, Ginther OJ. Changes in vascular perfusion of the endometrium in association with changes in location of the embryonic vesicle in mares.. Biol Reprod 2005 Mar;72(3):755-61.
    doi: 10.1095/biolreprod.104.036384pubmed: 15576822google scholar: lookup
  37. Silva LA, Klein C, Ealy AD, Sharp DC. Conceptus-mediated endometrial vascular changes during early pregnancy in mares: an anatomic, histomorphometric, and vascular endothelial growth factor receptor system immunolocalization and gene expression study.. Reproduction 2011 Oct;142(4):593-603.
    doi: 10.1530/REP-11-0149pubmed: 21757474google scholar: lookup
  38. Smits K, Willems S, Van Steendam K, Van De Velde M, De Lange V, Ververs C, Roels K, Govaere J, Van Nieuwerburgh F, Peelman L, Deforce D, Van Soom A. Proteins involved in embryo-maternal interaction around the signalling of maternal recognition of pregnancy in the horse.. Sci Rep 2018 Mar 27;8(1):5249.
    doi: 10.1038/s41598-018-23537-6pmc: PMC5869742pubmed: 29588480google scholar: lookup
  39. Tayade C, Fang Y, Hilchie D, Croy BA. Lymphocyte contributions to altered endometrial angiogenesis during early and midgestation fetal loss.. J Leukoc Biol 2007 Oct;82(4):877-86.
    doi: 10.1189/jlb.0507330pubmed: 17634279google scholar: lookup
  40. Tolosa E, Rodrigues C, Brehmer A, Freitas-Neto A. Manual de técnicas para histologia normal e patológica.. São Paulo: Manole; 2003.
  41. Waclawik A, Kaczmarek MM, Blitek A, Kaczynski P, Ziecik AJ. Embryo-maternal dialogue during pregnancy establishment and implantation in the pig.. Mol Reprod Dev 2017 Sep;84(9):842-855.
    doi: 10.1002/mrd.22835pubmed: 28628266google scholar: lookup
  42. Waclawik A. Novel insights into the mechanisms of pregnancy establishment: regulation of prostaglandin synthesis and signaling in the pig.. Reproduction 2011 Sep;142(3):389-99.
    doi: 10.1530/REP-11-0033pubmed: 21677026google scholar: lookup
  43. Wilsher S, Gower S, Allen WR. Immunohistochemical localisation of progesterone and oestrogen receptors at the placental interface in mares during early pregnancy.. Anim Reprod Sci 2011 Dec;129(3-4):200-8.
    doi: 10.1016/j.anireprosci.2011.11.004pubmed: 22176887google scholar: lookup

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