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Cells2020; 9(5); doi: 10.3390/cells9051162

Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project.

Abstract: Limb wounds on horses are often slow to heal and are prone to developing exuberant granulation tissue (EGT) and close primarily through epithelialization, which results in a cosmetically inferior and non-durable repair. In contrast, wounds on the body heal rapidly and primarily through contraction and rarely develop EGT. Intravenous (IV) multipotent mesenchymal stromal cells (MSCs) are promising. They home and engraft to cutaneous wounds and promote healing in laboratory animals, but this has not been demonstrated in horses. Furthermore, the clinical safety of administering >1.00 × 108 allogeneic MSCs IV to a horse has not been determined. A proof-of-principle pilot project was performed with two horses that were administered 1.02 × 108 fluorescently labeled allogeneic cord blood-derived MSCs (CB-MSCs) following wound creation on the forelimb and thorax. Wounds and contralateral non-wounded skin were sequentially biopsied on days 0, 1, 2, 7, 14, and 33 and evaluated with confocal microscopy to determine presence of homing and engraftment. Results confirmed preferential homing and engraftment to wounds with persistence of CB-MSCs at 33 days following wound creation, without clinically adverse reactions to the infusion. The absence of overt adverse reactions allows further studies to determine effects of IV CB-MSCs on equine wound healing.
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Publication Date: 2020-05-08 PubMed ID: 32397125PubMed Central: PMC7290349DOI: 10.3390/cells9051162Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the process and consequence of injecting horses with a specific type of cell derived from cord blood – termed multipotent mesenchymal stromal cells (MSCs) – in efforts to aid the healing of limb wounds. Initial results suggest the cells are drawn to the wound site and remained there for an extended period, with no negative side effects observed.

Horse Wound Healing Challenges

  • Unlike wounds elsewhere on their bodies, wounds on horse limbs certainly develop excess granulation tissue and heal primarily through a process called epithelialization. This provides a cosmetically unattractive result and a repair that is not long-lasting.
  • Rapid healing in horses typically happens through contraction and rarely develops any unwanted issues like excessive granulation.
  • The researchers are interested in a type of cells called MSCs which show promise for efficient wound healing. These cells are known to find their way to the wound sites and help regenerate the damaged tissues in lab animals. However, this process hasn’t been fully tested and confirmed in horses.
  • The safe dosage of these cells, when given through an intravenous (IV) method, is also unknown in horses.

Pilot Study Overview

  • To investigate the potential of these cells in wound healing in horses, the team carried out a small-scale study on two horses with artificial wounds created on their limbs and thorax.
  • They infused more than 1.00 × 10 of the MSCs, derived from cord blood and labelled with a visible marker for easy detection, into the horses.
  • Biopsies were taken from the wounds and the skin not affected by wounds on a schedule. This included day 0 (before treatment), 1, 2, 7, 14, and a month following the wound creation.

Findings and Implications

  • The microscopy evaluation of the biopsied skin tissue samples confirmed the MSCs were able to detect the wound sites and homed in on them more than non-wounded areas.
  • These cells were still identifiable at the wound site a month following the wounds’ creation, suggesting they can maintain their presence for a long time.
  • The horses didn’t exhibit any noticeable harmful effects following the MSCs infusion, making it safe for continued exploration in larger trials.
  • These findings have paved the way for further research in using IV MSCs derived from cord blood to enhance wound healing in horses.

Cite This Article

APA
Mund SJK, Kawamura E, Awang-Junaidi AH, Campbell J, Wobeser B, MacPhee DJ, Honaramooz A, Barber S. (2020). Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project. Cells, 9(5). https://doi.org/10.3390/cells9051162

Publication

Cells
ISSN: 2073-4409
NlmUniqueID: 101600052
Country: Switzerland
Language: English
Volume: 9
Issue: 5

Researcher Affiliations

Mund, Suzanne J K
  • Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Kawamura, Eiko
  • WCVM Imaging Centre, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Awang-Junaidi, Awang Hazmi
  • Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Campbell, John
  • Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Wobeser, Bruce
  • Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
MacPhee, Daniel J
  • Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Honaramooz, Ali
  • Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Barber, Spencer
  • Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.

MeSH Terms

  • Administration, Intravenous
  • Animals
  • Biopsy
  • Extremities / pathology
  • Fetal Blood / cytology
  • Fluorescence
  • Horses
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Multipotent Stem Cells / cytology
  • Pilot Projects
  • Skin / pathology
  • Wound Healing
  • Wounds and Injuries / pathology
  • Wounds and Injuries / therapy

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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