Horse agglutinins in infectious mononueleosis. 3. riterion for differential diagnosis.
Abstract: One hundred infectious mononucleosis and the same number of non-infectious mononucleosis sera were studied to evaluate the sensitivity and specificity of horse erythrocytes in the diagnosis of infectious mononucleosis. Titres of horse agglutinins in infectious mononucleosis sera ranged from 28 to 7,168 with a geometric mean of 550, whereas the corresponding sheep agglutinin titres ranged from less than 7 to 3,584, with a geometric mean of 126. Horse agglutinin titres of non-infectious mononucleosis sera ranged from less than 7 to 896, with a geometric mean of 59. Infectious mononucleosis sera tested with horse erythrocytes before and after absorption with guinea pig kidney and beef erythrocytes showed patterns different from those of non-infectious mononucleosis sera, even when sheep agglutinin titres were too low for satisfactory evaluation. After absorption with guinea pig kidney, horse agglutinin titres of infectious mononucleosis sera were uniformly higher than in the corresponding serum absorbed with beef erythrocytes. This was not the case for non-infectious mononucleosis sera. Results confirm our previously expressed view that horse erythrocytes are preferable to sheep erythrocytes in the serological diagnosis of infectious mononucleosis.
Publication Date: 1968-09-01 PubMed ID: 5697368PubMed Central: PMC473877DOI: 10.1136/jcp.21.5.631Google Scholar: Lookup
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- Journal Article
- Antibodies
- Clinical Study
- Comparative Study
- Diagnosis
- Disease control
- Disease Diagnosis
- Disease Etiology
- Disease Management
- Disease Outbreaks
- Disease Surveillance
- Disease Transmission
- Disease Treatment
- Epidemiology
- Equine Health
- Erythrocytes
- Horses
- Immunology
- Infectious Disease
- Laboratory Methods
- Serology
- Veterinary Medicine
Summary
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The research examines the sensitivity and specificity of horse erythrocytes in diagnosing infectious mononucleosis compared with non-infectious mononucleosis, concluding that horse erythrocytes are more preferable for this purpose than sheep erythrocytes.
Study Methodology
- In this study, researchers evaluated 100 samples of infectious mononucleosis and the same number of non-infectious mononucleosis sera. The aim of the study was to measure and compare the reactivity, or agglutinin titres, in both horse and sheep erythrocytes when faced with these two types of mononucleosis.
- Agglutinins are substances that cause particles to coagulate or clump together. In this case, the researchers were measuring the clumping reaction of infectious and non-infectious mononucleosis sera to horse and sheep erythrocytes (red blood cells).
Study Findings
- The study found that the reaction of infectious mononucleosis with horse erythrocytes resulted in higher agglutinin titres compared to non-infectious mononucleosis.
- In addition, researchers observed patterns of interaction between infectious mononucleosis sera and horse erythrocytes, whether tested before or after absorption with guinea pig kidney and beef erythrocytes. These patterns differed from those created by non-infectious mononucleosis sera, even when the corresponding agglutinin titres of sheep were too low for satisfactory evaluation.
Implications of Findings
- The higher agglutinin titres in infectious mononucleosis sera, prompted by horse erythrocytes, seem to imply that horse red blood cells are more reactive to the pathogen causing infectious mononucleosis, and thus, may be a better biomarker for diagnosing the condition than sheep erythrocytes.
- This research confirms the previously expressed belief of the research team that horse erythrocytes are indeed preferable to sheep erythrocytes when it comes to serologically diagnosing infectious mononucleosis.
In conclusion, this study puts forward significant findings with implications for improvement in the diagnosis procedure of infectious mononucleosis.
Cite This Article
APA
Lee CL, Zandrew F, Davidsohn I.
(1968).
Horse agglutinins in infectious mononueleosis. 3. riterion for differential diagnosis.
J Clin Pathol, 21(5), 631-634.
https://doi.org/10.1136/jcp.21.5.631 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antibodies / analysis
- Cattle
- Diagnosis, Differential
- Erythrocytes / immunology
- Guinea Pigs
- Hemagglutination Tests
- Horses
- Infectious Mononucleosis / immunology
- Sheep
References
This article includes 3 references
- Lee CL, Davidsohn I, Panczyszyn O. Horse agglutinins in infectious mononucleosis. II. The spot test.. Am J Clin Pathol 1968 Jan;49(1):12-8.
- WILKINSON PC, CARMICHAEL DS. IMMUNOCHEMICAL CHARACTERIZATION AND SEROLOGIC BEHAVIOR OF ANTIBODIES AGAINST RED CELLS IN INFECTIOUS MONONUCLEOSIS.. J Lab Clin Med 1964 Oct;64:529-39.
- Beer P. THE HETEROPHILE ANTIBODIES IN INFECTIOUS MONONUCLEOSIS AND AFTER THE INJECTION OF SERUM.. J Clin Invest 1936 Nov;15(6):591-9.
Citations
This article has been cited 9 times.- Byrne A, Bush R, Johns F, Upadhyay K. Limited Utility of Serology and Heterophile Test in the Early Diagnosis of Epstein-Barr Virus Mononucleosis in a Child after Renal Transplantation. Medicines (Basel) 2020 Apr 22;7(4).
- . Tests for infectious mononucleosis. Br Med J 1980 May 10;280(6224):1153-4.
- Nikoskelainen J, Leikola J, Klemola E. IgM antibodies specific for Epstein-Barr virus in infectious mononucleosis without heterophil antibodies. Br Med J 1974 Oct 12;4(5936):72-5.
- Joncas JH, Gilker JC, Chagnon A. Limitations of immunofluorescence tests in the diagnosis of infectious mononucleosis. Can Med Assoc J 1974 Apr 6;110(7):793-4 passim.
- Horwitz CA, Polesky H, Stillman T, Ward PC, Henle G, Henle W. Persistent haemagglutination for infectious mononucleosis in rheumatoid arthritis. Br Med J 1973 Mar 10;1(5853):591-2.
- Joncas JH. Infectious mononucleosis: current status of candidate virus. Can Med Assoc J 1969 Jul 12;101(1):33-6.
- Steeper TA, Horwitz CA, Moore SB, Henle W, Henle G, Ellis R, Flynn PJ. Severe thrombocytopenia in Epstein-Barr virus-induced mononucleosis. West J Med 1989 Feb;150(2):170-3.
- Strickler JG, Movahed LA, Gajl-Peczalska KJ, Horwitz CA, Brunning RD, Weiss LM. Oligoclonal T cell receptor gene rearrangements in blood lymphocytes of patients with acute Epstein-Barr virus-induced infectious mononucleosis. J Clin Invest 1990 Oct;86(4):1358-63.
- Lennette ET, Henle G, Henle W, Horwitz CA. Heterophil antigen in bovine sera detectable by immune adherence hemagglutination with infectious mononucleosis sera. Infect Immun 1978 Mar;19(3):923-7.
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