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Home / Equine Research Bank / Int J Vet Sci Med / Horse serum potentiates cellular viability and improves indo...
International journal of veterinary science and medicine2023; 11(1); 94-105; doi: 10.1080/23144599.2023.2248805

Horse serum potentiates cellular viability and improves indomethacin-induced adipogenesis in equine subcutaneous adipose-derived stem cells (ASCs).

Abstract: Subcutaneous fat tissue is an accessible and abundant source of multipotent stem cells for cell therapy in regenerative medicine. Successful trilineage differentiation is required to define the stemness features of the obtained mesenchymal cells, and adipogenesis is a part of it. Since indomethacin is bound to serum albumin, replacing foetal bovine serum (FBS) with horse serum (HS) in adipogenic induction protocols would suppress its cytotoxic effect and reveal a better adipogenic potential in equine MSCs. The equine subcutaneous adipose-derived stem cells (ASCs) were separately induced in adipogenesis by three different concentrations of 3-isobutyl-1-methylxanthine, IBMX (0.5 mM; 0.25 mM and 0.1 mM) and indomethacin (0.1 mM; 0.05 mM and 0.02 mM) for 48 h. In contrast to the IBMX, indomethacin in all concentrations caused dramatic cellular detachment. Further, the same induction concentrations were used in FBS and HS conditions for adipogenic induction. The MTT assay revealed that the culture media supplemented with HS raised cellular vitality by about 35% compared to those cultured in FBS. Based on those results, an adipogenic cocktail containing indomethacin (0.05 mM) and IBMX (0.5 mM), supplemented with HS and FBS, respectively, was applied for 18 days. The adiponectin gene expression was significantly up-regulated in HS-supplemented media since established changes in PPAR-gamma were insignificant. The tri-lineage differentiation was successful, and a cross-sectional area of adipocytes was performed. The albumin concentration was higher in HS than in FBS. In conclusion, our study revealed that HS is an appropriate supplement in induced adipogenesis since it probably suppresses the indomethacin-related cytotoxic effect and increases adipogenic ability in equine subcutaneous ASCs.
© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
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Publication Date: 2023-08-29 PubMed ID: 37655053PubMed Central: PMC10467519DOI: 10.1080/23144599.2023.2248805Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates ways to improve the viability and differentiation of horse fat-derived stem cells for use in regenerative medicine. They found that horse serum can boost cell vitality and aid in adipogenesis – the creation of fat cells – when using indomethacin, a drug commonly bound to serum albumin.

Objectives and Methodology

  • The study aims to assess the impact of horse serum (HS) on the adipogenic potential of subcutaneous adipose-derived stem cells (ASCs) in horses, particularly in the context of indomethacin-induced adipogenesis.
  • The researchers extracted ASCs from the subcutaneous fat tissues of horses – these are a readily available and versatile source of stem cells valuable for cell therapy and regenerative medicine.
  • Indomethacin, a drug associated with serum albumin, and 3-isobutyl-1-methylxanthine (IBMX), were used in different concentrations to induce adipogenesis in separate groups of ASCs.
  • ASCs were subjected to adipogenic induction in both foetal bovine serum (FBS) and HS conditions, utilising different concentrations of IBMX and indomethacin.

      • Results

      • The researchers observed that indomethacin, at all concentrations, resulted in substantial cellular detachment. This was not seen with IBMX.
      • Interestingly, adipogenic induction in a culture medium supplemented with HS increased cellular vitality by about 35% compared to those cultured in FBS.
      • A cocktail containing indomethacin and IBMX, supplemented with HS or FBS, was then applied for 18 days. They found that the gene expression of adiponectin, an important protein in the regulation of fat cells, was significantly increased in the group supplemented with HS.
      • Moreover, a successful tri-lineage differentiation was noted and the increased area of adipocytes was observed. The concentration of albumin was found higher in HS than in FBS.

      Conclusion

      • The study concludes that HS is a promising supplement in induced adipogenesis, as it appears to alleviate indomethacin-associated cytotoxicity and enhance adipogenic potential in equine subcutaneous ASCs. This paves the way for more efficient stem cell use in regenerative medicine.

Cite This Article

APA
Petrova V, Yonkova P, Simeonova G, Vachkova E. (2023). Horse serum potentiates cellular viability and improves indomethacin-induced adipogenesis in equine subcutaneous adipose-derived stem cells (ASCs). Int J Vet Sci Med, 11(1), 94-105. https://doi.org/10.1080/23144599.2023.2248805

Publication

International journal of veterinary science and medicine
ISSN: 2314-4599
NlmUniqueID: 101626221
Country: United States
Language: English
Volume: 11
Issue: 1
Pages: 94-105

Researcher Affiliations

Petrova, Valeria
  • Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, , Trakia UniversityStara Zagora, Bulgaria.
Yonkova, Penka
  • Department of Veterinary Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, , Trakia UniversityStara Zagora, Bulgaria.
Simeonova, Galina
  • Department of Veterinary Surgery, Faculty of Veterinary Medicine, , Trakia UniversityStara Zagora, Bulgaria.
Vachkova, Ekaterina
  • Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, , Trakia UniversityStara Zagora, Bulgaria.

Conflict of Interest Statement

The authors declare no conflict of interest.No potential conflict of interest was reported by the author(s).

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