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Home / Equine Research Bank / Acta Vet Scand / Hyaluronan turnover in the synovial fluid in metacarpophalan...
Acta veterinaria Scandinavica1996; 37(2); 147-151; doi: 10.1186/BF03548107

Hyaluronan turnover in the synovial fluid in metacarpophalangeal–and middle carpal joints in standardbred horses.

Abstract: The biological turnover of hyaluronan (sodium hyaluronate) of different molecular weights (0.6 x 10(6) and 2.5 x 10(6) Daltons) was studied in the synovial fluid of the middle carpal and metacarpophalangeal joints of 6 clinically healthy Standardbred horses. The hyaluronan was radioactively labelled with 14C. The biological half-life (t1/2) was calculated from repeated synovial samples after injection of the labelled hyaluronan. The mean t1/2 in the metacarpophalangeal joints was 9.7 h for low molecular weight hyaluronan and 8.9 h for high molecular weight hyaluronan and in the middle carpal joints 16.0 h and 12.5 h respectively. These was no significant difference in turnover of the different molecular weights of hyaluronan.
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Publication Date: 1996-01-01 PubMed ID: 8767693PubMed Central: PMC8064002DOI: 10.1186/BF03548107Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the biological turnover of hyaluronan in the synovial fluid of two joints in healthy Standardbred horses, finding no significant difference in turnover time between different molecular weights of hyaluronan.

Objective of the Research

  • This research aims to study the biological turnover of hyaluronan in the synovial fluid of the metacarpophalangeal and middle carpal joints of Standardbred horses. It specifically looks at hyaluronan of two different molecular weights, to see if this has any significant impact on the turnover rate.

Methods

  • The research was carried out on 6 clinically healthy Standardbred horses.
  • The molecule hyaluronan, with two different molecular weights – 0.6 x 10^6 and 2.5 x 10^6 Daltons – was radioactively labelled with 14C and injected into the joints of these horses. The molecular weight is essentially a measure of the size of the hyaluronan molecule.
  • The biological half-life (t1/2), or the time it takes for just half of the hyaluronan molecule to be biochemically processed and removed from the body, was then calculated. This was done by taking repeated samples of the synovial fluid after the injection and monitoring the degradation of the labelled hyaluronan.

Results

  • The research found that the mean half-life in the metacarpophalangeal joints was 9.7 hours for low molecular weight hyaluronan and 8.9 hours for high molecular weight hyaluronan. Meanwhile, in the middle carpal joints, the half-lives were 16.0 hours and 12.5 hours, respectively.
  • Crucially, the study found that there was no significant difference in the turnover of hyaluronan based on its molecular weight.

Conclusions

  • Based on the study’s results, it seems that the size of the hyaluronan molecule – whether it is of high or low molecular weight – does not significantly impact the time it takes for the molecule to be processed and expelled from the body of a healthy Standardbred horse.
  • This contributes to our understanding of hyaluronan’s biological turnover, which can help in developing treatments for joint injuries or diseases that involve changes in synovial fluid composition.

Cite This Article

APA
Lindholm A, Ronéus B, Lindblad G, Jones B. (1996). Hyaluronan turnover in the synovial fluid in metacarpophalangeal–and middle carpal joints in standardbred horses. Acta Vet Scand, 37(2), 147-151. https://doi.org/10.1186/BF03548107

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 37
Issue: 2
Pages: 147-151

Researcher Affiliations

Lindholm, A
  • Department of Medicine and Surgery, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden. Arne.Lindholm@kirmed.slu.se
Ronéus, B
    Lindblad, G
      Jones, B

        MeSH Terms

        • Animals
        • Carpus, Animal
        • Female
        • Horses
        • Hyaluronic Acid / chemistry
        • Hyaluronic Acid / metabolism
        • Male
        • Metacarpophalangeal Joint
        • Molecular Weight
        • Synovial Fluid / metabolism

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        Citations

        This article has been cited 4 times.
        1. Neuenschwander HM, Moreira JJ, Vendruscolo CP, Fülber J, Seidel SRT, Michelacci YM, Baccarin RYA. Hyaluronic acid has chondroprotective and joint-preserving effects on LPS-induced synovitis in horses. J Vet Sci 2019 Nov;20(6):e67.
          doi: 10.4142/jvs.2019.20.e67pubmed: 31775194google scholar: lookup
        2. Rivera F. Single intra-articular injection of high molecular weight hyaluronic acid for hip osteoarthritis. J Orthop Traumatol 2016 Mar;17(1):21-6.
          doi: 10.1007/s10195-015-0381-8pubmed: 26449357google scholar: lookup
        3. Chin L, Calabro A, Rodriguez ER, Tan CD, Walker E, Derwin KA. Characterization of and host response to tyramine substituted-hyaluronan enriched fascia extracellular matrix. J Mater Sci Mater Med 2011 Jun;22(6):1465-77.
          doi: 10.1007/s10856-011-4325-4pubmed: 21553156google scholar: lookup
        4. Coleman PJ, Scott D, Mason RM, Levick JR. Role of hyaluronan chain length in buffering interstitial flow across synovium in rabbits. J Physiol 2000 Jul 15;526 Pt 2(Pt 2):425-34.
          doi: 10.1111/j.1469-7793.2000.00425.xpubmed: 10896731google scholar: lookup
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