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Tissue & cell2004; 36(6); 431-438; doi: 10.1016/j.tice.2004.07.003

Hyaluronic acid and autologous synovial fluid induce chondrogenic differentiation of equine mesenchymal stem cells: a preliminary study.

Abstract: Mesenchymal stem cells (MSC) have the potential to differentiate into distinct mesenchymal tissues including cartilage, which suggest these cells as an attractive cell source for cartilage tissue engineering approaches. Our objective was to study the effects of TGF-beta1, hyaluronic acid and synovial fluid on chondrogenic differentiation of equine MSC. For that, bone marrow was aspirated from the tibia of one 18-month-old horse (Haflinger) and MSC were isolated using percoll-density centrifugation. To promote chondrogenesis, MSC were centrifuged to form a micromass and were cultured in a medium containing 10 ng/ml TGF-beta1 or 0.1mg/ml hyaluronic acid (Hylartil, Ostenil) or either 5%, 10% or 50% autologous synovial fluid as the chondrogenesis inducing factor. Differentiation along the chondrogenic lineage was documented by type II collagen and proteoglycan expression. MSC induced by TGF-beta1 alone showed the highest proteoglycan expression. Combining TGF-beta1 with hyaluronic acid could not increase the proteoglycan expression. Cultures stimulated by autologous synovial fluid (independent of concentration) and hyaluronic acid demonstrated a pronounced, but lower proteoglycan expression than cultures stimulated by TGF-beta1. The expression of cartilage-specific type II collagen was high and about the same in all stimulated cultures. In summary, hyaluronic acid and autologous synovial fluid induces chondrogenesis of equine mesenchymal stem cells, which encourage tissue engineering applications of MSC in chondral defects, as the natural environment in the joint is favorable for chondrogenic differentiation.
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Publication Date: 2004-11-10 PubMed ID: 15533458DOI: 10.1016/j.tice.2004.07.003Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how transforming growth factor-beta 1 (TGF-beta1), hyaluronic acid, and synovial fluid impact the development of cartilage from horse mesenchymal stem cells. The findings suggest that both hyaluronic acid and synovial fluid can promote this cartilage formation, indicating their potential usefulness for tissue engineering in treating cartilage defects.

Research objective and methods

  • The primary objective of this research was to understand the impact of TGF-beta1, hyaluronic acid, and synovial fluid on the differentiation of mesenchymal stem cells (MSC) into cartilage.
  • Mesenchymal stem cells were retrieved from the bone marrow of an 18-month-old horse.
  • The MSC were then segregated using a procedure called percoll-density centrifugation.
  • To provoke the development of cartilage — a process known as chondrogenesis — the MSC were formed into a micro-mass and cultured in a variety of mediums, each containing different concentrations of TGF-beta1, hyaluronic acid or synovial fluid.

Research outcomes

  • The results reveal that MSC stimulated by TGF-beta1 alone exhibited the highest production of proteoglycan, an integral part of cartilage.
  • When TGF-beta1 and hyaluronic acid were combined, there was no significant increase in proteoglycan production compared to when TGF-beta1 was used alone.
  • Both hyaluronic acid and synovial fluid, regardless of its concentration, triggered a lower, yet still substantial, production of proteoglycan compared to TGF-beta1.
  • Unchanging high expression levels of type II collagen, a cartilage-specific component, were observed in all cultures irrespective of the substance used for stimulation.

Conclusion and implications

  • The combined findings showed that hyaluronic acid and synovial fluid indeed instigate the formation of cartilage from horse mesenchymal stem cells.
  • This plays encouragingly into the field of tissue engineering, where such methods could be potentially utilised for treatment of cartilage defects.
  • One key implication is that the natural joint environment, rich in synovial fluid and hyaluronic acid, is a beneficial setting for cartilage development from MSC.

Cite This Article

APA
Hegewald AA, Ringe J, Bartel J, Krüger I, Notter M, Barnewitz D, Kaps C, Sittinger M. (2004). Hyaluronic acid and autologous synovial fluid induce chondrogenic differentiation of equine mesenchymal stem cells: a preliminary study. Tissue Cell, 36(6), 431-438. https://doi.org/10.1016/j.tice.2004.07.003

Publication

Tissue & cell
ISSN: 0040-8166
NlmUniqueID: 0214745
Country: Scotland
Language: English
Volume: 36
Issue: 6
Pages: 431-438

Researcher Affiliations

Hegewald, A A
  • Tissue Engineering Laboratory, Department of Rheumatology, Charité-University Medicine Berlin, Campus Mitte, Berlin, Germany.
Ringe, J
    Bartel, J
      Krüger, I
        Notter, M
          Barnewitz, D
            Kaps, C
              Sittinger, M

                MeSH Terms

                • Animals
                • Bone Marrow Cells / cytology
                • Bone Marrow Cells / drug effects
                • Cells, Cultured
                • Chondrogenesis / drug effects
                • Chondrogenesis / physiology
                • Collagen Type II / metabolism
                • Horses
                • Hyaluronic Acid / pharmacology
                • Immunohistochemistry
                • Mesenchymal Stem Cells / cytology
                • Mesenchymal Stem Cells / drug effects
                • Proteoglycans / metabolism
                • Synovial Fluid / physiology
                • Transforming Growth Factor beta / pharmacology
                • Transforming Growth Factor beta1

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