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Home / Equine Research Bank / Animals (Basel) / Identification and Quantification of Transient Receptor Pote...
Animals : an open access journal from MDPI2020; 10(3); 506; doi: 10.3390/ani10030506

Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue.

Abstract: Joint pain and osteoarthritis (OA) are some of the most common causes of lameness in horses, and most of the available treatments focus on symptomatic relief without a disease-modifying effect. TRPV1 is a potential target for treating joint diseases, including OA, and the present study aims to investigate if the TRPV1 receptor is present in equine articular tissue and determine whether the number of receptors is upregulated in joint inflammation. Metacarpo/metatarsophalangeal (MCP/MTP) joints from 15 horses euthanised for reasons unrelated to this study were included. Based on synovial fluid analysis, macroscopic evaluation, and magnetic resonance imaging (MRI), joints were divided into two groups: healthy joints and joints with pathology. ELISA analysis was performed on synovial tissue harvested from all joints. TPRV1 was found in all joints. The mean concentration of TRPV1 compared to total protein in healthy joints (8.4 × 10 ng/mL) and joints with pathology (12.9 × 10 ng/mL) differed significantly ( = 0.01, -test with Welch correction). Quantitative real-time reverse transcriptase PCR analysis was performed on RNA isolates from synovial tissue from all joints. TRPV1 mRNA expression ratio normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in healthy joints (0.16 (SD: 0.19)) and joints with pathology (0.24 (SD: 0.14)) did not differ significantly ( = 0.43, -test with Welch correction). mRNA expression of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-) was very low for both groups. In conclusion, TRPV1 was detected both on mRNA and the protein level, with a higher expression of TRPV1 in samples from joints with pathology. Future studies will determine the clinical potential of equine TRPV1 as a target in the management of joint pain and inflammation.
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Publication Date: 2020-03-18 PubMed ID: 32197454PubMed Central: PMC7143842DOI: 10.3390/ani10030506Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the presence and concentration of Transient Receptor Potential Vanilloid 1 (TRPV1) in joint tissue of horses, assessing whether the number of these receptors increases when the joint is inflamed. The findings suggest greater expression of TRPV1 in samples from inflamed joints, implying potential for TRPV1 as a treatment target for joint pain and inflammation in horses.

Research Methodology and Samples

  • The study utilized Metacarpo/metatarsophalangeal (MCP/MTP) joints from 15 horses euthanized due to reasons unrelated to this research.
  • These joints were categorized into two groups based on magnetic resonance imaging (MRI), synovial fluid analysis, and macroscopic evaluation. These categories were healthy joints and joints with pathology (disease).
  • The research team collected synovial tissue from all joints for ELISA analysis.

Investigation and Analysis

  • The presence of TRPV1 was identified in all tested joints.
  • When the concentration of TRPV1 was compared to total protein, a difference was observed between healthy joints (8.4 × 10 ng/mL) and joints with pathology (12.9 × 10 ng/mL).
  • Focusing on RNA isolates from synovial tissue, the researchers conducted a quantitative real-time reverse transcriptase PCR analysis.
  • The TRPV1 mRNA expression ratio (normalized to glyceraldehyde 3-phosphate dehydrogenase or GAPDH) did not show a significant difference between healthy joints and those with pathology.
  • Additionally, mRNA expression of inflammatory markers interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-) was found to be very low in both groups.

Conclusion and Future Implications

  • Throughout this study, TRPV1 was detected at both mRNA and protein levels, with a higher expression found in samples from inflamed or pathological joints.
  • The findings suggest that TRPV1 may serve as a new target for treatments aimed at controlling joint pain and inflammation in horses.
  • However, further studies are required to definitively establish the clinical potential of equine TRPV1 in the management of such conditions.

Cite This Article

APA
Braucke AFGV, Frederiksen NL, Berg LC, Aarsvold S, Müller FC, Boesen MP, Lindegaard C. (2020). Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue. Animals (Basel), 10(3), 506. https://doi.org/10.3390/ani10030506

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 3
PII: 506

Researcher Affiliations

Braucke, Anne Franck Gallagher Vom
  • Large Animal Teaching Hospital, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Frederiksen, Nanna Lysemose
  • Large Animal Teaching Hospital, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Berg, Lise Charlotte
  • Large Animal Teaching Hospital, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.
Aarsvold, Stacie
  • Puchalski Equine Imaging, CA, Petaluma, CA-94954, USA.
Müller, Felix Christoph
  • Department of Radiology, Herlev and Gentofte Hospital, DK-2730 Herlev, Denmark.
Boesen, Mikael Ploug
  • Department of Radiology, Copenhagen University Hospital Bispebjerg and Frederiksberg, DK-2400 Copenhagen NV, Denmark.
Lindegaard, Casper
  • Large Animal Teaching Hospital, Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, DK-2630 Taastrup, Denmark.

Conflict of Interest Statement

The authors declare no conflict of interest.

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