Identification of Y chromosome genetic variations in Chinese indigenous horse breeds.
Abstract: Y chromosome acts as a single nonrecombining unit that is male specific and in effect haploid, thus ensuring the preservation of mutational events as a single haplotype via male lines. In this study, 6 Y chromosome-specific microsatellites (SSR) were tested for the patrilineal genetic variations of 573 male samples from Chinese domestic horse (30 breeds), Przewalski's horse, and donkey. All the 6 loci appeared as a haplotype block in Przewalski's horse and the domestic donkey. There were notable differences, however, at Y chromosome markers between horse and donkey. There were 2 haplotypes of Eca.YA16 in the domestic horse breeds, Haplotype A (Allele A: 156 bp) and Haplotype B (Allele B: 152 bp). Allele A was the common allele among 30 horse breeds, and Allele B was found in 11 horse breeds. This is the first description of a Y chromosome variant for horses. The 2 haplotypes of Y chromosome discovered in the domestic horse breeds in China could be helpful in unveiling their intricate genetic genealogy.
Publication Date: 2010-05-23 PubMed ID: 20497969DOI: 10.1093/jhered/esq047Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research studied Y chromosome variations in Chinese indigenous horse breeds. It discovered two different haplotypes of one Y chromosome gene, which could help understand the genetic genealogy of these breeds.
Y Chromosome and Its Role
- The Y chromosome, present only in males, acts as a single, non-recombining unit. This means it does not mix with other chromosomes during reproduction, unlike most other chromosomes. Therefore, any mutations on the Y chromosome are preserved as a haplotype (a group of genes inherited together from a single parent) through male lineage.
Objectives of the Study
- The main purpose of this study was to discover and understand the patrilineal genetic variations in Chinese indigenous horse breeds. This could offer insights into their genetic history.
Methodology of the Study
- The researchers tested six Y chromosome-specific microsatellites, which are stretches of DNA with short, repeated sequences, in 573 male samples. These samples came from 30 Chinese domestic horse breeds, Przewalski’s horse, and donkeys.
Findings of the Study
- All six microsatellites appeared as a single haplotype block in Przewalski’s horse and donkeys, indicating a consistent inherited genetic trait.
- However, there were significant differences observed at the Y chromosome markers between horses and donkeys, indicating that these species have different male lineage histories.
- Within the horse breeds, there were two distinct haplotypes of the Eca.YA16, named Haplotype A and Haplotype B. Haplotype A (Allele A: 156 base pairs) was common among 30 horse breeds, while Haplotype B (Allele B: 152 base pairs) was found in 11 horse breeds. These differences in haplotype suggest the presence of distinct male lineages within the horse breeds in China.
Implications of the Study
- This is the first study that describes a Y chromosome variant in horses. Knowledge of these two genetic variants could provide valuable insight into the genetic origins and relationships of Chinese indigenous horse breeds.
Cite This Article
APA
Ling Y, Ma Y, Guan W, Cheng Y, Wang Y, Han J, Jin D, Mang L, Mahmut H.
(2010).
Identification of Y chromosome genetic variations in Chinese indigenous horse breeds.
J Hered, 101(5), 639-643.
https://doi.org/10.1093/jhered/esq047 Publication
Researcher Affiliations
- Animal Genetic Resources Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
MeSH Terms
- Alleles
- Animals
- China
- Equidae / genetics
- Genetic Variation
- Haplotypes
- Horses / genetics
- Male
- Microsatellite Repeats
- Phylogeny
- Y Chromosome / genetics
Citations
This article has been cited 19 times.- Giontella A, Cardinali I, Sarti FM, Silvestrelli M, Lancioni H. Y-Chromosome Haplotype Report among Eight Italian Horse Breeds. Genes (Basel) 2023 Aug 9;14(8).
- Sheikh A. Mitochondrial DNA sequencing of Kehilan and Hamdani horses from Saudi Arabia. Saudi J Biol Sci 2023 Sep;30(9):103741.
- Bozlak E, Radovic L, Remer V, Rigler D, Allen L, Brem G, Stalder G, Castaneda C, Cothran G, Raudsepp T, Okuda Y, Moe KK, Moe HH, Kounnavongsa B, Keonouchanh S, Van NH, Vu VH, Shah MK, Nishibori M, Kazymbet P, Bakhtin M, Zhunushov A, Paul RC, Dashnyam B, Nozawa K, Almarzook S, Brockmann GA, Reissmann M, Antczak DF, Miller DC, Sadeghi R, von Butler-Wemken I, Kostaras N, Han H, Manglai D, Abdurasulov A, Sukhbaatar B, Ropka-Molik K, Stefaniuk-Szmukier M, Lopes MS, da Câmara Machado A, Kalashnikov VV, Kalinkova L, Zaitev AM, Novoa-Bravo M, Lindgren G, Brooks S, Rosa LP, Orlando L, Juras R, Kunieda T, Wallner B. Refining the evolutionary tree of the horse Y chromosome. Sci Rep 2023 Jun 2;13(1):8954.
- Cardinali I, Giontella A, Tommasi A, Silvestrelli M, Lancioni H. Unlocking Horse Y Chromosome Diversity. Genes (Basel) 2022 Dec 2;13(12).
- Klecel W, Martyniuk E. From the Eurasian Steppes to the Roman Circuses: A Review of Early Development of Horse Breeding and Management. Animals (Basel) 2021 Jun 22;11(7).
- Kusliy MA, Vorobieva NV, Tishkin AA, Makunin AI, Druzhkova AS, Trifonov VA, Iderkhangai TO, Graphodatsky AS. Traces of Late Bronze and Early Iron Age Mongolian Horse Mitochondrial Lineages in Modern Populations. Genes (Basel) 2021 Mar 12;12(3).
- Liu S, Fu C, Yang Y, Zhang Y, Ma H, Xiong Z, Ling Y, Zhao C. Current genetic conservation of Chinese indigenous horses revealed with Y-chromosomal and mitochondrial DNA polymorphisms. G3 (Bethesda) 2021 Feb 9;11(2).
- Liu S, Yang Y, Pan Q, Sun Y, Ma H, Liu Y, Wang M, Zhao C, Wu C. Ancient Patrilineal Lines and Relatively High ECAY Diversity Preserved in Indigenous Horses Revealed With Novel Y-Chromosome Markers. Front Genet 2020;11:467.
- Raudsepp T, Finno CJ, Bellone RR, Petersen JL. Ten years of the horse reference genome: insights into equine biology, domestication and population dynamics in the post-genome era. Anim Genet 2019 Dec;50(6):569-597.
- Ma H, Wu Y, Xiang H, Yang Y, Wang M, Zhao C, Wu C. Some maternal lineages of domestic horses may have origins in East Asia revealed with further evidence of mitochondrial genomes and HVR-1 sequences. PeerJ 2018;6:e4896.
- Khaudov AD, Duduev AS, Kokov ZA, Amshokov KK, Zhekamukhov MK, Zaitsev AM, Reissmann M. Genetic analysis of maternal and paternal lineages in Kabardian horses by uniparental molecular markers. Open Vet J 2018;8(1):40-46.
- Reissmann M, Musa L, Zakizadeh S, Ludwig A. Distribution of coat-color-associated alleles in the domestic horse population and Przewalski's horse. J Appl Genet 2016 Nov;57(4):519-525.
- Han H, Zhang Q, Gao K, Yue X, Zhang T, Dang R, Lan X, Chen H, Lei C. Y-Single Nucleotide Polymorphisms Diversity in Chinese Indigenous Horse. Asian-Australas J Anim Sci 2015 Aug;28(8):1066-74.
- Brandariz-Fontes C, Leonard JA, Vega-Pla JL, Backström N, Lindgren G, Lippold S, Rico C. Y-chromosome analysis in Retuertas horses. PLoS One 2013;8(5):e64985.
- Wallner B, Vogl C, Shukla P, Burgstaller JP, Druml T, Brem G. Identification of genetic variation on the horse y chromosome and the tracing of male founder lineages in modern breeds. PLoS One 2013;8(4):e60015.
- Petersen JL, Mickelson JR, Cothran EG, Andersson LS, Axelsson J, Bailey E, Bannasch D, Binns MM, Borges AS, Brama P, da Câmara Machado A, Distl O, Felicetti M, Fox-Clipsham L, Graves KT, Guérin G, Haase B, Hasegawa T, Hemmann K, Hill EW, Leeb T, Lindgren G, Lohi H, Lopes MS, McGivney BA, Mikko S, Orr N, Penedo MC, Piercy RJ, Raekallio M, Rieder S, Røed KH, Silvestrelli M, Swinburne J, Tozaki T, Vaudin M, M Wade C, McCue ME. Genetic diversity in the modern horse illustrated from genome-wide SNP data. PLoS One 2013;8(1):e54997.
- Warmuth V, Eriksson A, Bower MA, Barker G, Barrett E, Hanks BK, Li S, Lomitashvili D, Ochir-Goryaeva M, Sizonov GV, Soyonov V, Manica A. Reconstructing the origin and spread of horse domestication in the Eurasian steppe. Proc Natl Acad Sci U S A 2012 May 22;109(21):8202-6.
- Lippold S, Knapp M, Kuznetsova T, Leonard JA, Benecke N, Ludwig A, Rasmussen M, Cooper A, Weinstock J, Willerslev E, Shapiro B, Hofreiter M. Discovery of lost diversity of paternal horse lineages using ancient DNA. Nat Commun 2011 Aug 23;2:450.
- Cieslak M, Pruvost M, Benecke N, Hofreiter M, Morales A, Reissmann M, Ludwig A. Origin and history of mitochondrial DNA lineages in domestic horses. PLoS One 2010 Dec 20;5(12):e15311.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
