IHH gene polymorphism among three horse breeds and its application for association test in horses with osteochondrosis.
Abstract: Genetic polymorphism of IHH gene were investigated in Angloarabian, Polish Coldblood and Polish Halfbred horses with the inclusion of a group of Polish Halfbreds affected by osteochondrosis. IHH is a good candidate gene for association study of developmental disorders mainly affecting skeleton development. DNA sequence spanning IHH gene annotated in the horse genome and its putative promoter were investigated using SANGER sequencing. Analysis of genetic variability at polymorphic sites in the IHH gene body and the promoter region confirmed genetic differences between warmblood and coldblood horse breeds. A test for allelic and genotypic association at particular SNP sites revealed no association with osteochondrosis in investigated group of Polish Halfbreds. It was concluded that participation of different warmblood breeds in pedigrees of Polish Halfbreds make it difficult to search for genetic variants being associated with this complex disorder in this breed. IHH gene polymorphism investigated among three different horse populations would be valuable for further studies on equine bone developmental disorders.
© 2013 The Authors.
Publication Date: 2013-07-08 PubMed ID: 23865964DOI: 10.1111/j.1601-5223.2013.02282.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examines the genetic variability of the IHH gene in three horse breeds and its potential association with osteochondrosis, a joint and bone disorder. It concludes that while there are genetic differences between warmblood and coldblood horse breeds, the IHH gene polymorphism does not appear to be associated with osteochondrosis in the tested Polish Halfbreds.
About the Research
- The study investigates the genetic polymorphism of the Indian Hedgehog (IHH) gene among three horse breeds – Angloarabian, Polish Coldblood, and Polish Halfbreds. A particular group of Polish Halfbreds suffering from osteochondrosis is included for further investigation.
- The IHH gene is a key gene of interest since it is thought to play a significant role in bone and joint development. Hence, it is a promising candidate for studying developmental disorders that primarily affect the skeletal system, including osteochondrosis.
Methodology
- The researchers used a scientifically accepted method called SANGER sequencing to investigate the DNA sequence spanning the IHH gene and its presumptive promoter – a segment of DNA which initiates gene transcription.
Results and Discussion
- The study found variabilities in genes, or genetic polymorphism, at certain sites of the IHH gene body and the promoter region. The results confirmed that there were indeed genetic differences between the two types of horse breeds – the warmblood (Polish Halfbreds and Angloarabian) and the coldblood (Polish Coldblood).
- However, the researchers found no association between the allelic and genotypic variants of the IHH gene at specific SNP (Single Nucleotide Polymorphism) sites and osteochondrosis in the studied group of Polish Halfbreds. In simpler terms, the variations of the IHH gene do not appear to influence the disorder.
- The researchers concluded that the mixed parentage of different warmblood breeds in the lineage or pedigrees of Polish Halfbreds makes it challenging to find genetic variants associated with complex disorders like osteochondrosis.
Implications of the Research
- This research’s findings provide an invaluable foundation for further investigations on equine bone developmental disorders. Even though the IHH gene polymorphism wasn’t associated with osteochondrosis in this study, it could potentially be useful for exploring other genetic variants or even other bone-related disorders in horses.
Cite This Article
APA
Zabek T, Golonka P, Fornal A, Semik E.
(2013).
IHH gene polymorphism among three horse breeds and its application for association test in horses with osteochondrosis.
Hereditas, 150(2-3), 38-43.
https://doi.org/10.1111/j.1601-5223.2013.02282.x Publication
Researcher Affiliations
- Laboratory of Genomics of National Research Institute of Animal Production, Balice, Poland. tomasz.zabek@izoo.krakow.pl
MeSH Terms
- Alleles
- Animals
- Genetic Variation
- Genotype
- Haplotypes
- Hedgehog Proteins / genetics
- Horse Diseases / diagnosis
- Horse Diseases / genetics
- Horses / genetics
- Microsatellite Repeats
- Osteochondrosis / diagnosis
- Osteochondrosis / genetics
- Osteochondrosis / veterinary
- Polymorphism, Genetic
- Promoter Regions, Genetic
- Sequence Analysis, DNA
- Species Specificity
Citations
This article has been cited 2 times.- Mendoza L, Piquemal D, Lejeune JP, Vander Heyden L, Noguier F, Bruno R, Sandersen C, Serteyn D. Age-dependent expression of osteochondrosis-related genes in equine leukocytes. Vet Rec Open 2015;2(1):e000058.
- Cui J, Wang H, Liu S, Zhu L, Qiu X, Jiang Z, Wang X, Liu Z. SNP discovery from transcriptome of the swimbladder of Takifugu rubripes. PLoS One 2014;9(3):e92502.
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