Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses.
Abstract: An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48) and a recombinant truncated B. equi merozoite antigen 2 (rEMA-2t). An evaluation of the ability of the BceICT to detect antibodies in sera from uninfected horses and experimentally infected horses showed high sensitivities and specificities of 83.3% (10/12 sera) and 92.9% (52/56 sera), respectively, for the anti-B. caballi antibody and 94.1% (16/17 sera) and 88.2% (45/51 sera), respectively, for the anti-B. equi antibody. Results from the detection of antibodies in field-collected sera indicated that the BceICT results corresponded with those of enzyme-linked immunosorbent assays (ELISA), showing 91.8% correspondence (67/73 sera) for B. caballi and 95.9% correspondence (70/73 sera) for B. equi, and that the BceICT results also corresponded with the ICT for B. caballi and for B. equi, both of which were 98.2% (55/56 sera). The comparable results of the ICT and ELISA and the simplicity and rapidity of the performance of the ICT suggest that the BceICT would be a feasible test for the simultaneous serodiagnosis of both agents of equine babesiosis in the field.
Publication Date: 2006-05-10 PubMed ID: 16682475PubMed Central: PMC1459648DOI: 10.1128/CVI.13.5.553-555.2006Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Antibodies
- Diagnosis
- Diagnostic Technique
- Disease
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Equine Health
- Equine Science
- Horses
- Immunology
- Infection
- Piroplasmosis
- Serodiagnosis
- Serological Surveys
- Seroprevalence
- Theileria equi
- Tick-Borne Diseases
- Veterinary Medicine
- Veterinary Research
Summary
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The research article presents a new test for the concurrent detection of antibodies specific to Babesia caballi and B. equi in horses. The test, BceICT, uses recombinant proteins to identify these antibodies. Compared to other tests, the BceICT showed high sensitivity and specificity, proving to be a felicitous tool for diagnosing equine babesiosis in the field.
Introduction to Immunochromatographic Test
- The research discusses the development of an immunochromatographic test (BceICT) to simultaneously detect B. caballi and B. equi specific antibodies.
- The test uses a B. caballi 48-kDa rhoptry protein (rBc48) and a truncated B. equi merozoite antigen 2 (rEMA-2t) – both are recombinant proteins, i.e., proteins generated in a lab.
Evaluation of BceICT
- The team evaluated the ability of the BceICT to detect antibodies using serum samples from uninfected and experimentally infected horses.
- Findings show that the BceICT has high sensitivities (ability to correctly identify positives) and specificities (ability to correctly identify negatives). For the anti-B. caballi antibody, the sensitivities and specificities were 83.3% and 92.9% respectively, and 94.1% and 88.2% respectively for the anti-B. equi antibody.
- This indicates that the BceICT is highly effective in correctly identifying both infected and non-infected samples.
Comparison with ELISA
- The team compared the BceICT results with the ones from enzyme-linked immunosorbent assays (ELISA), which is a widely used method of measuring antibodies in blood.
- The BceICT and ELISA showed a 91.8% correspondence for B. caballi and a 95.9% correspondence for B. equi.
- Moreover, the BceICT’s results also closely matched the results of another ICT for B. caballi and B. equi, both of which were 98.2%.
Conclusion
- Based on the results, the BceICT is not only comparable to ELISA and other ICT methods, but it’s also simpler and faster.
- Therefore, the research concludes that the BceICT could be an effective test for the simultaneous diagnosis of B. caballi and B. equi infections in horses in field conditions.
Cite This Article
APA
Huang X, Xuan X, Verdida RA, Zhang S, Yokoyama N, Xu L, Igarashi I.
(2006).
Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses.
Clin Vaccine Immunol, 13(5), 553-555.
https://doi.org/10.1128/CVI.13.5.553-555.2006 Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Antigens, Protozoan / immunology
- Babesia / immunology
- Babesiosis / diagnosis
- Babesiosis / immunology
- Babesiosis / veterinary
- Chromatography / methods
- Endemic Diseases / veterinary
- Enzyme-Linked Immunosorbent Assay
- Horse Diseases / blood
- Horse Diseases / diagnosis
- Horse Diseases / immunology
- Horses
- Immunoassay / methods
- Protozoan Proteins / immunology
- Serologic Tests / methods
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Citations
This article has been cited 4 times.- Lira-Amaya JJ, Martínez-García G, Santamaria-Espinosa RM, Castañeda-Arriola RO, Ojeda-Carrasco JJ, Ávila-Ramírez G, Figueroa-Millán JV. Comparative Study of Indirect Fluorescent Antibody, ELISA, and Immunochromatography Tests for Serological Diagnosis of Bovine Babesiosis Caused by Babesia bovis.. Animals (Basel) 2021 Nov 24;11(12).
- Barshevskaya LV, Sotnikov DV, Zherdev AV, Khassenov BB, Baltin KK, Eskendirova SZ, Mukanov KK, Mukantayev KK, Dzantiev BB. Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia.. Biosensors (Basel) 2019 Sep 29;9(4).
- Mosqueda J, Olvera-Ramirez A, Aguilar-Tipacamu G, Canto GJ. Current advances in detection and treatment of babesiosis.. Curr Med Chem 2012;19(10):1504-18.
- An DJ, Kim TY, Song DS, Kang BK, Park BK. An immunochromatography assay for rapid antemortem diagnosis of dogs suspected to have canine distemper.. J Virol Methods 2008 Feb;147(2):244-9.
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