Immunocytochemical localization of lysozyme and beta-defensin in the apocrine glands of the equine scrotum.
Abstract: The present study revealed in detail the subcellular localization of lysozyme and beta-defensin in the apocrine glands of the equine scrotal skin, a specific body region. The apocrine glandular cells were equipped with a varying number of secretory granules, a well-developed Golgi apparatus and abundant cisternae of the rough endoplasmic reticulum within their cytoplasm. In these cells, reactive gold particles representing lysozyme were detectable in the secretory granules as well as the Golgi apparatus and elements of the rough endoplasmic reticulum. Additionally, the antimicrobial peptide group of beta-defensin was also localized in the above-mentioned ultrastructures of the secretory cells. The presence and secretion of such substances that may serve as a non-specific defense against microorganisms are suggestive of the protective effect of the secretory production elaborated by the apocrine glands.
Publication Date: 2007-07-17 PubMed ID: 17639436DOI: 10.1007/s00403-007-0766-5Google Scholar: Lookup
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- Journal Article
Summary
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This study explores the presence and location of lysozyme and beta-defensin, substances with protective properties against microorganisms, in horse scrotal glands.
Introductory Explanation
- The research focuses on the immunocytochemical positioning of two substances: lysozyme and beta-defensin, within the apocrine glands of the equine scrotal skin. Greater detail is provided about their subcellular localization in this specific body region.
Key Findings
- It is demonstrated that the apocrine glandular cells contain numerous secretory granules, a well-formed Golgi apparatus, and many elements of the rough endoplic reticulum.
- The researchers discovered the presence of lysozyme, represented by reactive gold particles, both in the secretory granules and in the other cell structures.
- Further to this, the research also revealed the positioning of the beta-defensin group, which is an antimicrobial peptide, within the cells of the scrotal glands.
Significance of the Research
- The research generated important insight about these substances and their localization in the equine scrotal skin’s apocrine glands.
- Their presence can be linked to the protective effects against microbial organisms these glands provide.
- This suggests a non-specific defensive mechanism the apocrine glands possess against microorganisms, due to the secretory functions they exhibit involving lysozyme and beta-defensin.
Cite This Article
APA
Yasui T, Fukui K, Nara T, Habata I, Meyer W, Tsukise A.
(2007).
Immunocytochemical localization of lysozyme and beta-defensin in the apocrine glands of the equine scrotum.
Arch Dermatol Res, 299(8), 393-397.
https://doi.org/10.1007/s00403-007-0766-5 Publication
Researcher Affiliations
- Department of Veterinary Anatomy, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, 252-8510, Japan. yasui@brs.nihon-u.ac.jp
MeSH Terms
- Animals
- Apocrine Glands / immunology
- Apocrine Glands / metabolism
- Apocrine Glands / ultrastructure
- Endoplasmic Reticulum, Rough / metabolism
- Endoplasmic Reticulum, Rough / ultrastructure
- Golgi Apparatus / metabolism
- Golgi Apparatus / ultrastructure
- Horses
- Immunohistochemistry
- Male
- Microscopy, Immunoelectron
- Muramidase / metabolism
- Scrotum / immunology
- Scrotum / metabolism
- beta-Defensins / metabolism
Citations
This article has been cited 4 times.- Van Cleemput J, Poelaert KCK, Laval K, Vanderheijden N, Dhaenens M, Daled S, Boyen F, Pasmans F, Nauwynck HJ. An Alphaherpesvirus Exploits Antimicrobial β-Defensins To Initiate Respiratory Tract Infection. J Virol 2020 Mar 31;94(8).
- Yasui T, Gomi H, Kitahara T, Tsukise A. Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands. Eur J Histochem 2017 Aug 8;61(3):2828.
- Nara T, Yasui T, Fujimori O, Meyer W, Tsukise A. Histochemical properties of sialic acids and antimicrobial substances in canine anal glands. Eur J Histochem 2011;55(3):e29.
- Bruhn O, Grötzinger J, Cascorbi I, Jung S. Antimicrobial peptides and proteins of the horse--insights into a well-armed organism. Vet Res 2011 Sep 2;42(1):98.
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