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Immunodiagnosis of Trichinella infection in the horse.
Abstract: From 1998 to 2000, 5,267 horse sera were collected from several Trichinella regions in Romania. Sera were initially screened in laboratories in Romania, Serbia and Italy with an ELISA and a Western blot (Wb) using an excretory/secretory (ES) antigen and several conjugates (protein A, protein G, and sheep or goat anti-horse). Differences in serology results were obtained among the different conjugates and also between ELISA and Wb. Depending on the test used, specific antibodies were found at a prevalence rate of 3-6% of horses. Serum samples classified as positive were tested again by ELISA using a synthetic tyvelose glycan-BSA antigen, in Italy. All serum samples tested using this antigen were negative; in contrast, serum samples from experimentally infected horses were positive with the glycan antigen. The negative results obtained with the glycan antigen are consistent with the low prevalence of horse trichinellosis reported in the literature. Based on these results, further studies are needed to validate immunodiagnostic tests to detect Trichinella infection in horses.
Publication Date: 2001-08-04 PubMed ID: 11484374DOI: 10.1051/parasite/200108s2260Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research focuses on determining the prevalence and diagnosis methods of Trichinella infection (a type of parasitic infection) in horses, mainly in Romania. The study found inconsistencies in tests using different conjugates and between ELISA and Western blot tests. All serum tests using a synthetic antigen were negative, supporting the low reported prevalence of horse trichinellosis, though further studies are needed for valid immunodiagnostic tests.
Objective
- The purpose of this study was to use ELISA and Western blot tests to detect Trichinella infections in horses located in several regions in Romania having Trichinella. The results were intended to lend insight into the prevalence of this infection among the horse population and ascertain the effectiveness of different testing methods.
Methodology
- 5,267 horse sera were collected from Romania between 1998 and 2000. These samples were examined using ELISA and Western blot tests in various laboratories in Romania, Serbia, and Italy.
- The tests utilized an excretory/secretory (ES) antigen and several different conjugates – protein A, protein G, and sheep or goat anti-horse.
Findings
- Discrepancies were noted in the serology results among different conjugates, and between ELISA and Western blot tests.
- The prevalence rate of specific antibodies reflecting Trichinella infection varied between 3 to 6% in horses, contingent on the test used.
- Serum samples validated as positive were retested in Italy, utilizing ELISA and synthetic tyvelose glycan-BSA antigen. All serum samples tested negative with this antigen.
- In contrast, serum samples sourced from experimentally infected horses were positive with the glycan antigen.
Conclusion
- The negative results achieved with the glycan antigen are in line with the low prevalence rates of horse trichinellosis documented in prior research.
- The research suggests that more studies are essential to verify the reliability of immunodiagnostic tests used to detect Trichinella infections in horses.
Cite This Article
APA
Sofronic-Milosavljevic L, Pozio E, Patrascu IV, Skerovic N, Gomez Morales MA, Gamble HR.
(2001).
Immunodiagnosis of Trichinella infection in the horse.
Parasite, 8(2 Suppl), S260-S262.
https://doi.org/10.1051/parasite/200108s2260 Publication
Researcher Affiliations
- Institute for the Application of Nuclear Energy (INEP), Banatska 31B, 11080 Belgrade, Yugoslavia. l.sofronic@alfort.afssa.fr
MeSH Terms
- Animals
- Antibodies, Helminth / blood
- Antigens, Helminth / immunology
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- Horse Diseases / diagnosis
- Horse Diseases / epidemiology
- Horse Diseases / immunology
- Horses
- Romania / epidemiology
- Trichinellosis / diagnosis
- Trichinellosis / epidemiology
- Trichinellosis / veterinary
Citations
This article has been cited 4 times.- Abdugheni R, Li L, Yang ZN, Huang Y, Fang BZ, Shurigin V, Mohamad OAA, Liu YH, Li WJ. Microbial Risks Caused by Livestock Excrement: Current Research Status and Prospects. Microorganisms 2023 Jul 27;11(8).
- Elghandour MMMY, Maggiolino A, Vázquez-Mendoza P, Alvarado-Ramírez ER, Cedillo-Monroy J, De Palo P, Salem AZM. Moringa oleifera as a Natural Alternative for the Control of Gastrointestinal Parasites in Equines: A Review. Plants (Basel) 2023 May 8;12(9).
- Lönker NS, Fechner K, Wahed AAE. Horses as a Crucial Part of One Health. Vet Sci 2020 Feb 29;7(1).
- Bien J, Näreaho A, Varmanen P, Gozdzik K, Moskwa B, Cabaj W, Nyman TA, Savijoki K. Comparative analysis of excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting. Proteome Sci 2012 Feb 11;10(1):10.
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