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Home / Equine Research Bank / J Clin Microbiol / Immunodiffusion test for diagnosing and monitoring pythiosis...
Journal of clinical microbiology1986; 23(5); 813-816; doi: 10.1128/jcm.23.5.813-816.1986

Immunodiffusion test for diagnosing and monitoring pythiosis in horses.

Abstract: A practical, sensitive, and specific immunodiffusion test was developed for diagnosing and monitoring pythiosis in horses. Culture filtrates, a soluble cell mass, and trypsinized Pythium sp. antigens were evaluated against prepared rabbit anti-Pythium sp. serum and pythiosis horse case sera. The culture filtrate antigens demonstrated the greatest capacity for detecting precipitins and the greatest stability during storage. In contrast, the trypsinized antigens had the weakest capability for detecting multiple precipitins and the poorest stability. The 13 sera from horses with proven active pythiosis were positive in immunodiffusion tests with the culture filtrate antigens. Each serum contained from three to six precipitins. Treated horses lost precipitins, and some became antibody negative. No false-positive reactions were noted in tests with sera from normal horses and humans or with sera from a variety of heterologous horse and human infections.
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Publication Date: 1986-05-01 PubMed ID: 3086368PubMed Central: PMC268727DOI: 10.1128/jcm.23.5.813-816.1986Google Scholar: Lookup
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  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers have developed a highly efficient and specific immunodiffusion test to diagnose and monitor pythiosis, a serious infection in horses. They evaluated different types of antigens and found that those produced from culture filtrates were the most effective and stored the best, allowing for accurate and stable testing.

Understanding Immunodiffusion Test

  • An immunodiffusion test is a method used in laboratories to detect the presence of antibodies in a sample of serum – the clear liquid obtained from blood.
  • In this study, the immunodiffusion test was developed specifically to diagnose and monitor pythiosis, a severe fungal infection in horses that can cause lesions in their gastrointestinal tract and skin.
  • The process involves the reaction of antibodies in the serum sample with specific antigens, which are substances that illicit an immune response in the body. If the antibodies are present, they will bind to the antigens, forming visible lines of precipitation that can be used to confirm diagnosis.

Evaluating Antigens

  • The researchers tested three types of antigens: culture filtrates, a soluble cell mass, and trypsinized Pythium sp. antigens.
  • Culture filtrate antigens displayed the highest capacity to detect precipitins – the visible clumps formed when antibodies bind to antigens – and the greatest stability during storage. This makes them the most practical for use in lab testing.
  • In contrast, trypsinized antigens were less efficient at detecting multiple precipitins and had poor stability.

Interpreting Results

  • The researchers found that all 13 sera from horses with active pythiosis showed positive results in immunodiffusion tests using the culture filtrate antigens. Each serum sample contained between three to six precipitins.
  • Treatment appears to reduce the presence of these precipitines, and in some cases, the horses became antibody negative – suggesting they had recovered from the infection.
  • The tests produced no false-positive reactions when used with sera from healthy horses and humans, or with sera from horses and humans with other types of infections, indicating a high level of specificity in diagnosing pythiosis.

Cite This Article

APA
Mendoza L, Kaufman L, Standard PG. (1986). Immunodiffusion test for diagnosing and monitoring pythiosis in horses. J Clin Microbiol, 23(5), 813-816. https://doi.org/10.1128/jcm.23.5.813-816.1986

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 23
Issue: 5
Pages: 813-816

Researcher Affiliations

Mendoza, L
    Kaufman, L
      Standard, P G

        MeSH Terms

        • Animals
        • Antigens, Fungal / analysis
        • Chytridiomycota / immunology
        • Horse Diseases / diagnosis
        • Horses
        • Immunodiffusion
        • Mycoses / diagnosis
        • Mycoses / veterinary
        • Pythium / immunology

        References

        This article includes 2 references
        1. Miller RI, Campbell RS. Immunological studies on equine phycomycosis.. Aust Vet J 1982 Jun;58(6):227-31.
          pubmed: 6814414doi: 10.1111/j.1751-0813.1982.tb00682.xgoogle scholar: lookup
        2. Austwick PK, Copland JW. Swamp cancer.. Nature 1974 Jul 5;250(461):84.
          pubmed: 4276247doi: 10.1038/250084a0google scholar: lookup

        Citations

        This article has been cited 19 times.
        1. Chechi JL, Rotchanapreeda T, da Paz GS, Prado AC, Oliveira AL, Vieira JCS, Buzalaf MAR, Rodrigues AM, Santos LDD, Krajaejun T, Bosco SMG. Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis.. J Fungi (Basel) 2021 May 28;7(6).
          doi: 10.3390/jof7060423pubmed: 34071174google scholar: lookup
        2. Perrino C, Ferdinandy P, Bøtker HE, Brundel BJJM, Collins P, Davidson SM, den Ruijter HM, Engel FB, Gerdts E, Girao H, Gyöngyösi M, Hausenloy DJ, Lecour S, Madonna R, Marber M, Murphy E, Pesce M, Regitz-Zagrosek V, Sluijter JPG, Steffens S, Gollmann-Tepeköylü C, Van Laake LW, Van Linthout S, Schulz R, Ytrehus K. Improving translational research in sex-specific effects of comorbidities and risk factors in ischaemic heart disease and cardioprotection: position paper and recommendations of the ESC Working Group on Cellular Biology of the Heart.. Cardiovasc Res 2021 Jan 21;117(2):367-385.
          doi: 10.1093/cvr/cvaa155pubmed: 32484892google scholar: lookup
        3. Jaturapaktrarak C, Payattikul P, Lohnoo T, Kumsang Y, Laikul A, Pathomsakulwong W, Yurayart C, Tonpitak W, Krajaejun T. Protein A/G-based enzyme-linked immunosorbent assay for detection of anti-Pythium insidiosum antibodies in human and animal subjects.. BMC Res Notes 2020 Mar 6;13(1):135.
          doi: 10.1186/s13104-020-04981-ypubmed: 32143691google scholar: lookup
        4. Inkomlue R, Larbcharoensub N, Karnsombut P, Lerksuthirat T, Aroonroch R, Lohnoo T, Yingyong W, Santanirand P, Sansopha L, Krajaejun T. Development of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosis.. J Clin Microbiol 2016 Jan;54(1):43-8.
          doi: 10.1128/JCM.02113-15pubmed: 26719582google scholar: lookup
        5. Salas Y, Márquez A, Canelón J, Perazzo Y, Colmenárez V, López JA. Equine pythiosis: report in crossed bred (Criole Venezuelan) horses.. Mycopathologia 2012 Dec;174(5-6):511-7.
          doi: 10.1007/s11046-012-9562-7pubmed: 22772508google scholar: lookup
        6. Botton SA, Pereira DI, Costa MM, Azevedo MI, Argenta JS, Jesus FP, Alves SH, Santurio JM. Identification of Pythium insidiosum by nested PCR in cutaneous lesions of Brazilian horses and rabbits.. Curr Microbiol 2011 Apr;62(4):1225-9.
          doi: 10.1007/s00284-010-9781-4pubmed: 21188592google scholar: lookup
        7. Krajaejun T, Keeratijarut A, Sriwanichrak K, Lowhnoo T, Rujirawat T, Petchthong T, Yingyong W, Kalambaheti T, Smittipat N, Juthayothin T, Sullivan TD. The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase.. Clin Vaccine Immunol 2010 Aug;17(8):1203-10.
          doi: 10.1128/CVI.00515-09pubmed: 20237199google scholar: lookup
        8. Jindayok T, Piromsontikorn S, Srimuang S, Khupulsup K, Krajaejun T. Hemagglutination test for rapid serodiagnosis of human pythiosis.. Clin Vaccine Immunol 2009 Jul;16(7):1047-51.
          doi: 10.1128/CVI.00113-09pubmed: 19494087google scholar: lookup
        9. Krajaejun T, Imkhieo S, Intaramat A, Ratanabanangkoon K. Development of an immunochromatographic test for rapid serodiagnosis of human pythiosis.. Clin Vaccine Immunol 2009 Apr;16(4):506-9.
          doi: 10.1128/CVI.00276-08pubmed: 19225072google scholar: lookup
        10. Bosco Sde M, Bagagli E, Araújo JP Jr, Candeias JM, de Franco MF, Alencar Marques ME, Mendoza L, de Camargo RP, Alencar Marques S. Human pythiosis, Brazil.. Emerg Infect Dis 2005 May;11(5):715-8.
          doi: 10.3201/eid1105.040943pubmed: 15890126google scholar: lookup
        11. Schurko AM, Mendoza L, de Cock AW, Bedard JE, Klassen GR. Development of a species-specific probe for Pythium insidiosum and the diagnosis of pythiosis.. J Clin Microbiol 2004 Jun;42(6):2411-8.
          doi: 10.1128/JCM.42.6.2411-2418.2004pubmed: 15184412google scholar: lookup
        12. Krajaejun T, Kunakorn M, Niemhom S, Chongtrakool P, Pracharktam R. Development and evaluation of an in-house enzyme-linked immunosorbent assay for early diagnosis and monitoring of human pythiosis.. Clin Diagn Lab Immunol 2002 Mar;9(2):378-82.
          doi: 10.1128/cdli.9.2.378-382.2002pubmed: 11874882google scholar: lookup
        13. Mendoza L, Kaufman L, Mandy W, Glass R. Serodiagnosis of human and animal pythiosis using an enzyme-linked immunosorbent assay.. Clin Diagn Lab Immunol 1997 Nov;4(6):715-8.
          doi: 10.1128/cdli.4.6.715-718.1997pubmed: 9384295google scholar: lookup
        14. De Cock AW, Mendoza L, Padhye AA, Ajello L, Kaufman L. Pythium insidiosum sp. nov., the etiologic agent of pythiosis.. J Clin Microbiol 1987 Feb;25(2):344-9.
          doi: 10.1128/jcm.25.2.344-349.1987pubmed: 3818928google scholar: lookup
        15. Mendoza L, Kaufman L, Standard P. Antigenic relationship between the animal and human pathogen Pythium insidiosum and nonpathogenic Pythium species.. J Clin Microbiol 1987 Nov;25(11):2159-62.
          doi: 10.1128/jcm.25.11.2159-2162.1987pubmed: 3121666google scholar: lookup
        16. Imwidthaya P, Srimuang S. Immunodiffusion test for diagnosing human pythiosis.. Mycopathologia 1989 May;106(2):109-12.
          doi: 10.1007/BF00437089pubmed: 2507920google scholar: lookup
        17. Pracharktam R, Changtrakool P, Sathapatayavongs B, Jayanetra P, Ajello L. Immunodiffusion test for diagnosis and monitoring of human pythiosis insidiosi.. J Clin Microbiol 1991 Nov;29(11):2661-2.
          doi: 10.1128/jcm.29.11.2661-2662.1991pubmed: 1774283google scholar: lookup
        18. Mendoza L, Nicholson V, Prescott JF. Immunoblot analysis of the humoral immune response to Pythium insidiosum in horses with pythiosis.. J Clin Microbiol 1992 Nov;30(11):2980-3.
          doi: 10.1128/jcm.30.11.2980-2983.1992pubmed: 1452669google scholar: lookup
        19. Mendoza L, Villalobos J, Calleja CE, Solis A. Evaluation of two vaccines for the treatment of pythiosis insidiosi in horses.. Mycopathologia 1992 Aug;119(2):89-95.
          doi: 10.1007/BF00443939pubmed: 1435952google scholar: lookup
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