Immunohistochemical analysis of equine pulmonary granular cell tumours.
Abstract: Histopathological and immunohistochemical examinations were made on four female horses aged 9-12 years with pulmonary granular cell tumours (GCTs). The tumours, which were multiple, of varying size, firm and off-white in colour, surrounded the bronchi and bronchioles. Metastatic lesions were not detected. The tumour cells had abundant eosinophilic cytoplasm filled with prominent coarse eosinophilic granules. Immunohistochemically, these tumour cells reacted uniformly with vimentin and S100 antibodies. Most were immunolabelled by antibodies against glial fibrillary acidic protein (GFAP), myelin basic protein (MBP) and protein gene product 9.5 (PGP9.5), and a few cells were positive with Leu7 antibody. However, the tumour cells did not react with antibodies against neurofilament protein (NF), cytokeratin (CK), chromogranin, alpha1 antichymotrypsin (AACT), myoglobin, desmin, alpha-actin or alpha-smooth muscle actin (alpha-SMA). These immunohistochemical properties of tumour cells support the hypothesis that equine pulmonary GCTs are derived from Schwann cells of the peripheral nervous system in peribronchial and peribronchiolar tissues. GFAP, MBP, Leu7 and PGP9.5 antibodies should help to distinguish equine granular cell tumours from other tumours.
Copyright Harcourt Publishers Ltd.
Publication Date: 2001-02-27 PubMed ID: 11222008DOI: 10.1053/jcpa.2000.0439Google Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research explores the histopathological and immunohistochemical characteristics of pulmonary granular cell tumors (GCTs) in horses aged 9-12 years. The findings suggest these tumors may originate from Schwann cells of the peripheral nervous system.
Overview of the Study
- This study is a detailed examination of pulmonary granular cell tumors (GCTs) in four female horses aged between 9 and 12 years.
- The primary focus is on the histopathological (the study of changes in tissues caused by disease) and immunohistochemical (the process of detecting antigens in cells of a tissue section) properties of these tumors.
Characteristics of the Tumors
- The tumors observed in these horses were multiple, varied in size, and appeared firm and off-white.
- They were found surrounding the bronchi and bronchioles, which are parts of the respiratory system in horses.
- No metastatic lesions, or instances of the disease spreading from the primary site to other areas, were detected.
Immunohistochemical Analysis
- The researchers looked at how tumor cells reacted to various antibodies, proteins that the immune system uses to neutralize pathogens such as pathogenic bacteria and viruses.
- The tumor cells reacted uniformly with vimentin and S100 antibodies. Most of the cells were immunolabeled by antibodies against glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), and protein gene product 9.5 (PGP9.5), and a few cells showed a positive reaction with Leu7 antibody.
- However, the tumor cells did not react with antibodies against several other proteins.
Significance of the Findings
- These immunohistochemical properties of the tumor cells led the researchers to support the hypothesis that equine pulmonary GCTs are derived from Schwann cells of the peripheral nervous system, which are located in peribronchial and peribronchiolar tissues.
- The discovery of these specific reactions to GFAP, MBP, Leu7, and PGP9.5 antibodies could serve as a distinguishing tool to differentiate equine granular cell tumors from other types of tumors in horses.
Cite This Article
APA
Kagawa Y, Hirayama K, Tagami M, Tsunoda N, Yoshino T, Matsui T, Furuoka H, Taniyama H.
(2001).
Immunohistochemical analysis of equine pulmonary granular cell tumours.
J Comp Pathol, 124(2-3), 122-127.
https://doi.org/10.1053/jcpa.2000.0439 Publication
Researcher Affiliations
- Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Bunkyodai-Midorimachi 582-1, Ebetsu, Hokkaido 069-8501, Japan.
MeSH Terms
- Animals
- Biomarkers, Tumor / metabolism
- Female
- Granular Cell Tumor / chemistry
- Granular Cell Tumor / pathology
- Granular Cell Tumor / veterinary
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Humans
- Immunoenzyme Techniques / veterinary
- Lung Neoplasms / chemistry
- Lung Neoplasms / pathology
- Lung Neoplasms / veterinary
- Neoplasm Proteins / metabolism
- Species Specificity
Citations
This article has been cited 2 times.- Mayor C, Verdés J, Alomar J, Novellas R, Pumarola M, Añor S. Intracranial Granular Cell Tumours in Three Dogs: Atypical Magnetic Resonance Imaging Features and Immunohistochemical Study. Vet Sci 2023 Feb 9;10(2).
- Hayes DA, Kunde DA, Taylor RL, Pyecroft SB, Sohal SS, Snow ET. ERBB3: A potential serum biomarker for early detection and therapeutic target for devil facial tumour 1 (DFT1). PLoS One 2017;12(6):e0177919.
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